Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique

Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique

BACKGROUND AND OBJECTIVE: Acinetobacter (A.) baumannii is a gram-negative non-fermentative coccobacilli with increasing relevance in a variety of hospital-acquired infections. The aim of this study was to determine the frequency of bla-OXA-2 and bla-OXA-10 resistance genes and class I Integron among...

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Autores principales: A Rahimzadeh, S Farajnia, MA Pourbabaee, KH Ansarin, MR Zolfaghari, N Masoudi
Formato: article
Lenguaje:EN
FA
Publicado: Babol University of Medical Sciences 2012
Materias:
acinetobacter baumannii
extended-spectrum beta-lactamase
bla-oxa-2
bla-oxa-10
int-1
Medicine
R
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/c8737dfe825d4790848c27c5dbab0902
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spelling oai:doaj.org-article:c8737dfe825d4790848c27c5dbab09022021-11-10T08:53:26ZDetection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique1561-41072251-7170https://doaj.org/article/c8737dfe825d4790848c27c5dbab09022012-09-01T00:00:00Zhttp://jbums.org/article-1-4185-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Acinetobacter (A.) baumannii is a gram-negative non-fermentative coccobacilli with increasing relevance in a variety of hospital-acquired infections. The aim of this study was to determine the frequency of bla-OXA-2 and bla-OXA-10 resistance genes and class I Integron among Acinetobacter baumannii strains isolated from patients referred to Imam Reza hospital of Tabriz city, Iran.METHODS: This cross-sectional descriptive study was carried out on 100 Acinetobacter baumannii isolated from patients referred to Imam Reza hospital of Tabriz, Iran in 2009-2010. The isolates were identified using standard biochemical and microbiological tests. ESBL production was determined in isolates by Combined Disc test. The presence of bla-OXA-10, bla-OXA-2 and INT-1 genes in clinical isolates was investigated by PCR technique. FINDINGS: The results of antimicrobial sensitivity tests revealed that the highest resistance was against cefixime (100%), ceftizoxime (100%) and ticaracilin (100%), whereas the highest susceptibility was observed for polymyxin B (84%), colistin (77%) and rifampin (73%). Combined Disc Test showed that 60% of isolated were ESBL producer among them 7 cases (11.6%) were positive for bla-OXA-2 and 5 cases (8.3%) for bla-OXA-10 genes. Screening for INT-1 genes demonstrated that 73% of isolates were positive for Int-1 insertion sequence.CONCLUSION: The results of this study showed the presence of OXA-2 and OXA-10 type ESBLs and class I integron genes among drug resistant Acinetobacter strains that reminding the necessity of preventive measures for inhibiting dissemination of these resistant isolates.A Rahimzadeh,S Farajnia,MA Pourbabaee,KH Ansarin,MR Zolfaghari,N MasoudiBabol University of Medical Sciencesarticleacinetobacter baumanniiextended-spectrum beta-lactamasebla-oxa-2bla-oxa-10int-1MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 5, Pp 56-63 (2012)
institution DOAJ
collection DOAJ
language EN
FA
topic acinetobacter baumannii
extended-spectrum beta-lactamase
bla-oxa-2
bla-oxa-10
int-1
Medicine
R
Medicine (General)
R5-920
spellingShingle acinetobacter baumannii
extended-spectrum beta-lactamase
bla-oxa-2
bla-oxa-10
int-1
Medicine
R
Medicine (General)
R5-920
A Rahimzadeh,
S Farajnia,
MA Pourbabaee,
KH Ansarin,
MR Zolfaghari,
N Masoudi
Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique
description BACKGROUND AND OBJECTIVE: Acinetobacter (A.) baumannii is a gram-negative non-fermentative coccobacilli with increasing relevance in a variety of hospital-acquired infections. The aim of this study was to determine the frequency of bla-OXA-2 and bla-OXA-10 resistance genes and class I Integron among Acinetobacter baumannii strains isolated from patients referred to Imam Reza hospital of Tabriz city, Iran.METHODS: This cross-sectional descriptive study was carried out on 100 Acinetobacter baumannii isolated from patients referred to Imam Reza hospital of Tabriz, Iran in 2009-2010. The isolates were identified using standard biochemical and microbiological tests. ESBL production was determined in isolates by Combined Disc test. The presence of bla-OXA-10, bla-OXA-2 and INT-1 genes in clinical isolates was investigated by PCR technique. FINDINGS: The results of antimicrobial sensitivity tests revealed that the highest resistance was against cefixime (100%), ceftizoxime (100%) and ticaracilin (100%), whereas the highest susceptibility was observed for polymyxin B (84%), colistin (77%) and rifampin (73%). Combined Disc Test showed that 60% of isolated were ESBL producer among them 7 cases (11.6%) were positive for bla-OXA-2 and 5 cases (8.3%) for bla-OXA-10 genes. Screening for INT-1 genes demonstrated that 73% of isolates were positive for Int-1 insertion sequence.CONCLUSION: The results of this study showed the presence of OXA-2 and OXA-10 type ESBLs and class I integron genes among drug resistant Acinetobacter strains that reminding the necessity of preventive measures for inhibiting dissemination of these resistant isolates.
format article
author A Rahimzadeh,
S Farajnia,
MA Pourbabaee,
KH Ansarin,
MR Zolfaghari,
N Masoudi
author_facet A Rahimzadeh,
S Farajnia,
MA Pourbabaee,
KH Ansarin,
MR Zolfaghari,
N Masoudi
author_sort A Rahimzadeh,
title Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique
title_short Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique
title_full Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique
title_fullStr Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique
title_full_unstemmed Detection of Prevalence of OXA-2 and OXA-10 Type ESBL and Class I Integron among Acinetobacter Bumanii Strains Isolated from Patients of Tabriz City (Iran) by PCR Technique
title_sort detection of prevalence of oxa-2 and oxa-10 type esbl and class i integron among acinetobacter bumanii strains isolated from patients of tabriz city (iran) by pcr technique
publisher Babol University of Medical Sciences
publishDate 2012
url https://doaj.org/article/c8737dfe825d4790848c27c5dbab0902
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