Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops

Abstract Detailed analysis of the cells that infiltrate lesional skin cannot be performed in skin biopsy specimens using immunohistochemistry or cell separation techniques because enzyme treatments applied during the isolation step can destroy small amounts of protein and minor cell populations in t...

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Autores principales:	Kan Torii, Yukinori Okada, Akimichi Morita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c87888703e71481288fc6e2615ffee40
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spelling	oai:doaj.org-article:c87888703e71481288fc6e2615ffee402021-12-02T19:16:18ZDetermining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops10.1038/s41598-021-98804-02045-2322https://doaj.org/article/c87888703e71481288fc6e2615ffee402021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98804-0https://doaj.org/toc/2045-2322Abstract Detailed analysis of the cells that infiltrate lesional skin cannot be performed in skin biopsy specimens using immunohistochemistry or cell separation techniques because enzyme treatments applied during the isolation step can destroy small amounts of protein and minor cell populations in the biopsy specimen. Here, we describe a method for isolating T cells from drops of whole blood obtained from lesions during skin biopsy in patients with cutaneous T-cell lymphoma. Lesional blood is assumed to contain lesional resident cells, cells from capillary vessels, and blood overflowing from capillary vessels into the lesion area. The lesional blood showed substantial increases in distinct cell populations, chemokines, and the expression of various genes. The proportion of CD8+CD45RO+ T cells in the lesional blood negatively correlated with the modified severity-weighted assessment tool scores. CD4+CD45RO+ T cells in the lesional blood expressed genes associated with the development of cancer and progression of cutaneous T-cell lymphoma. In addition, CD8+CD45RO+ T cells in lesional blood had unique T-cell receptor repertoires in lesions of each stage. Assessment of lesional blood drops might provide new insight into the pathogenesis of mycosis fungoides and facilitate evaluation of the treatment efficacy for mycosis fungoides as well as other skin inflammatory diseases.Kan ToriiYukinori OkadaAkimichi MoritaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kan Torii Yukinori Okada Akimichi Morita Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops
description	Abstract Detailed analysis of the cells that infiltrate lesional skin cannot be performed in skin biopsy specimens using immunohistochemistry or cell separation techniques because enzyme treatments applied during the isolation step can destroy small amounts of protein and minor cell populations in the biopsy specimen. Here, we describe a method for isolating T cells from drops of whole blood obtained from lesions during skin biopsy in patients with cutaneous T-cell lymphoma. Lesional blood is assumed to contain lesional resident cells, cells from capillary vessels, and blood overflowing from capillary vessels into the lesion area. The lesional blood showed substantial increases in distinct cell populations, chemokines, and the expression of various genes. The proportion of CD8+CD45RO+ T cells in the lesional blood negatively correlated with the modified severity-weighted assessment tool scores. CD4+CD45RO+ T cells in the lesional blood expressed genes associated with the development of cancer and progression of cutaneous T-cell lymphoma. In addition, CD8+CD45RO+ T cells in lesional blood had unique T-cell receptor repertoires in lesions of each stage. Assessment of lesional blood drops might provide new insight into the pathogenesis of mycosis fungoides and facilitate evaluation of the treatment efficacy for mycosis fungoides as well as other skin inflammatory diseases.
format	article
author	Kan Torii Yukinori Okada Akimichi Morita
author_facet	Kan Torii Yukinori Okada Akimichi Morita
author_sort	Kan Torii
title	Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops
title_short	Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops
title_full	Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops
title_fullStr	Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops
title_full_unstemmed	Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops
title_sort	determining the immune environment of cutaneous t-cell lymphoma lesions through the assessment of lesional blood drops
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c87888703e71481288fc6e2615ffee40
work_keys_str_mv	AT kantorii determiningtheimmuneenvironmentofcutaneoustcelllymphomalesionsthroughtheassessmentoflesionalblooddrops AT yukinoriokada determiningtheimmuneenvironmentofcutaneoustcelllymphomalesionsthroughtheassessmentoflesionalblooddrops AT akimichimorita determiningtheimmuneenvironmentofcutaneoustcelllymphomalesionsthroughtheassessmentoflesionalblooddrops
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Determining the immune environment of cutaneous T-cell lymphoma lesions through the assessment of lesional blood drops

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