Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora

Abstract Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity to environmental extremes. The objective of the present study was to establish a foundation of genetic-based selection tools for beneficial traits of hea...

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Autores principales:	Noa Levy, Adi Faigenboim, Liora Salame, Carlos Molina, Ralf-Udo Ehlers, Itamar Glazer, Dana Ment
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c87ac4a707d84bc3a5836367fcb423c4
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spelling	oai:doaj.org-article:c87ac4a707d84bc3a5836367fcb423c42021-12-02T16:32:08ZCharacterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora10.1038/s41598-020-67097-02045-2322https://doaj.org/article/c87ac4a707d84bc3a5836367fcb423c42020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67097-0https://doaj.org/toc/2045-2322Abstract Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity to environmental extremes. The objective of the present study was to establish a foundation of genetic-based selection tools for beneficial traits of heat and desiccation tolerance in entomopathogenic nematodes. Screening of natural populations enabled us to create a diverse genetic and phenotypic pool. Gene expression patterns and genomic variation were studied in natural isolates. Heterorhabditis isolates were phenotyped by heat- and desiccation-stress bioassays to determine their survival rates compared to a commercial line. Transcriptomic study was carried out for the commercial line, a high heat-tolerant strain, and for the natural, low heat-tolerant isolate. The results revealed a higher number of upregulated vs. downregulated transcripts in both isolates vs. their respective controls. Functional annotation of the differentially expressed transcripts revealed several known stress-related genes and pathways uniquely expressed. Genome sequencing of isolates with varied degrees of stress tolerance indicated variation among the isolates regardless of their phenotypic characterization. The obtained data lays the groundwork for future studies aimed at identifying genes and molecular markers as genetic selection tools for enhancement of entomopathogenic nematodes ability to withstand environmental stress conditions.Noa LevyAdi FaigenboimLiora SalameCarlos MolinaRalf-Udo EhlersItamar GlazerDana MentNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noa Levy Adi Faigenboim Liora Salame Carlos Molina Ralf-Udo Ehlers Itamar Glazer Dana Ment Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
description	Abstract Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity to environmental extremes. The objective of the present study was to establish a foundation of genetic-based selection tools for beneficial traits of heat and desiccation tolerance in entomopathogenic nematodes. Screening of natural populations enabled us to create a diverse genetic and phenotypic pool. Gene expression patterns and genomic variation were studied in natural isolates. Heterorhabditis isolates were phenotyped by heat- and desiccation-stress bioassays to determine their survival rates compared to a commercial line. Transcriptomic study was carried out for the commercial line, a high heat-tolerant strain, and for the natural, low heat-tolerant isolate. The results revealed a higher number of upregulated vs. downregulated transcripts in both isolates vs. their respective controls. Functional annotation of the differentially expressed transcripts revealed several known stress-related genes and pathways uniquely expressed. Genome sequencing of isolates with varied degrees of stress tolerance indicated variation among the isolates regardless of their phenotypic characterization. The obtained data lays the groundwork for future studies aimed at identifying genes and molecular markers as genetic selection tools for enhancement of entomopathogenic nematodes ability to withstand environmental stress conditions.
format	article
author	Noa Levy Adi Faigenboim Liora Salame Carlos Molina Ralf-Udo Ehlers Itamar Glazer Dana Ment
author_facet	Noa Levy Adi Faigenboim Liora Salame Carlos Molina Ralf-Udo Ehlers Itamar Glazer Dana Ment
author_sort	Noa Levy
title	Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
title_short	Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
title_full	Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
title_fullStr	Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
title_full_unstemmed	Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
title_sort	characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of heterorhabditis bacteriophora
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c87ac4a707d84bc3a5836367fcb423c4
work_keys_str_mv	AT noalevy characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora AT adifaigenboim characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora AT liorasalame characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora AT carlosmolina characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora AT ralfudoehlers characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora AT itamarglazer characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora AT danament characterizationofthephenotypicandgenotypictolerancetoabioticstressesofnaturalpopulationsofheterorhabditisbacteriophora
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Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora

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