Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the exist...

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Autores principales:	Alexander G. Savelyev, Anastasia V. Sochilina, Roman A. Akasov, Anton V. Mironov, Alina Yu. Kapitannikova, Tatiana N. Borodina, Natalya V. Sholina, Kirill V. Khaydukov, Andrei V. Zvyagin, Alla N. Generalova, Evgeny V. Khaydukov
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hyaluronic acid flavin mononucleotide hollow-core fiber cell-laden hydrogel photopolymerization photofabrication Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/c889241634db4d678ba2547c41f939c0
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spelling	oai:doaj.org-article:c889241634db4d678ba2547c41f939c02021-12-03T06:55:46ZFacile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication2296-418510.3389/fbioe.2021.783834https://doaj.org/article/c889241634db4d678ba2547c41f939c02021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.783834/fullhttps://doaj.org/toc/2296-4185Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young’s modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry.Alexander G. SavelyevAlexander G. SavelyevAnastasia V. SochilinaAnastasia V. SochilinaRoman A. AkasovRoman A. AkasovRoman A. AkasovAnton V. MironovAlina Yu. KapitannikovaTatiana N. BorodinaNatalya V. SholinaNatalya V. SholinaKirill V. KhaydukovAndrei V. ZvyaginAndrei V. ZvyaginAndrei V. ZvyaginAlla N. GeneralovaAlla N. GeneralovaEvgeny V. KhaydukovEvgeny V. KhaydukovEvgeny V. KhaydukovFrontiers Media S.A.articlehyaluronic acidflavin mononucleotidehollow-core fibercell-laden hydrogelphotopolymerizationphotofabricationBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hyaluronic acid flavin mononucleotide hollow-core fiber cell-laden hydrogel photopolymerization photofabrication Biotechnology TP248.13-248.65
spellingShingle	hyaluronic acid flavin mononucleotide hollow-core fiber cell-laden hydrogel photopolymerization photofabrication Biotechnology TP248.13-248.65 Alexander G. Savelyev Alexander G. Savelyev Anastasia V. Sochilina Anastasia V. Sochilina Roman A. Akasov Roman A. Akasov Roman A. Akasov Anton V. Mironov Alina Yu. Kapitannikova Tatiana N. Borodina Natalya V. Sholina Natalya V. Sholina Kirill V. Khaydukov Andrei V. Zvyagin Andrei V. Zvyagin Andrei V. Zvyagin Alla N. Generalova Alla N. Generalova Evgeny V. Khaydukov Evgeny V. Khaydukov Evgeny V. Khaydukov Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
description	Bioprinting emerges as a powerful flexible approach for tissue engineering with prospective capability to produce tissue on demand, including biomimetic hollow-core fiber structures. In spite of significance for tissue engineering, hollow-core structures proved difficult to fabricate, with the existing methods limited to multistage, time-consuming, and cumbersome procedures. Here, we report a versatile cell-friendly photopolymerization approach that enables single-step prototyping of hollow-core as well as solid-core hydrogel fibers initially loaded with living cells. This approach was implemented by extruding cell-laden hyaluronic acid glycidyl methacrylate hydrogel directly into aqueous solution containing free radicals generated by continuous blue light photoexcitation of the flavin mononucleotide/triethanolamine photoinitiator. Diffusion of free radicals from the solution to the extruded structure initiated cross-linking of the hydrogel, progressing from the structure surface inwards. Thus, the cross-linked wall is formed and its thickness is limited by penetration of free radicals in the hydrogel volume. After developing in water, the hollow-core fiber is formed with centimeter range of lengths. Amazingly, HaCaT cells embedded in the hydrogel successfully go through the fabrication procedure. The broad size ranges have been demonstrated: from solid core to 6% wall thickness of the outer diameter, which was variable from sub-millimeter to 6 mm, and Young’s modulus ∼1.6 ± 0.4 MPa. This new proof-of-concept fibers photofabrication approach opens lucrative opportunities for facile three-dimensional fabrication of hollow-core biostructures with controllable geometry.
format	article
author	Alexander G. Savelyev Alexander G. Savelyev Anastasia V. Sochilina Anastasia V. Sochilina Roman A. Akasov Roman A. Akasov Roman A. Akasov Anton V. Mironov Alina Yu. Kapitannikova Tatiana N. Borodina Natalya V. Sholina Natalya V. Sholina Kirill V. Khaydukov Andrei V. Zvyagin Andrei V. Zvyagin Andrei V. Zvyagin Alla N. Generalova Alla N. Generalova Evgeny V. Khaydukov Evgeny V. Khaydukov Evgeny V. Khaydukov
author_facet	Alexander G. Savelyev Alexander G. Savelyev Anastasia V. Sochilina Anastasia V. Sochilina Roman A. Akasov Roman A. Akasov Roman A. Akasov Anton V. Mironov Alina Yu. Kapitannikova Tatiana N. Borodina Natalya V. Sholina Natalya V. Sholina Kirill V. Khaydukov Andrei V. Zvyagin Andrei V. Zvyagin Andrei V. Zvyagin Alla N. Generalova Alla N. Generalova Evgeny V. Khaydukov Evgeny V. Khaydukov Evgeny V. Khaydukov
author_sort	Alexander G. Savelyev
title	Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
title_short	Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
title_full	Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
title_fullStr	Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
title_full_unstemmed	Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication
title_sort	facile cell-friendly hollow-core fiber diffusion-limited photofabrication
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c889241634db4d678ba2547c41f939c0
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Facile Cell-Friendly Hollow-Core Fiber Diffusion-Limited Photofabrication

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