Primitive erythrocytes are generated from hemogenic endothelial cells

Abstract Primitive erythroblasts are the first blood cells generated during embryonic hematopoiesis. Tracking their emergence both in vivo and in vitro has remained challenging due to the lack of specific cell surface markers. To selectively investigate primitive erythropoiesis, we have engineered a...

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Autores principales:	Monika Stefanska, Kiran Batta, Rahima Patel, Magdalena Florkowska, Valerie Kouskoff, Georges Lacaud
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c88a97e8e87c4720ba4b1208235e6341
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spelling	oai:doaj.org-article:c88a97e8e87c4720ba4b1208235e63412021-12-02T12:32:25ZPrimitive erythrocytes are generated from hemogenic endothelial cells10.1038/s41598-017-06627-92045-2322https://doaj.org/article/c88a97e8e87c4720ba4b1208235e63412017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06627-9https://doaj.org/toc/2045-2322Abstract Primitive erythroblasts are the first blood cells generated during embryonic hematopoiesis. Tracking their emergence both in vivo and in vitro has remained challenging due to the lack of specific cell surface markers. To selectively investigate primitive erythropoiesis, we have engineered a new transgenic embryonic stem (ES) cell line, where eGFP expression is driven by the regulatory sequences of the embryonic βH1 hemoglobin gene expressed specifically in primitive erythroid cells. Using this ES cell line, we observed that the first primitive erythroblasts are detected in vitro around day 1.5 of blast colony differentiation, within the cell population positive for the early hematopoietic progenitor marker CD41. Moreover, we establish that these eGFP+ cells emerge from a hemogenic endothelial cell population similarly to their definitive hematopoietic counterparts. We further generated a corresponding βH1-eGFP transgenic mouse model and demonstrated the presence of a primitive erythroid primed hemogenic endothelial cell population in the developing embryo. Taken together, our findings demonstrate that both in vivo and in vitro primitive erythrocytes are generated from hemogenic endothelial cells.Monika StefanskaKiran BattaRahima PatelMagdalena FlorkowskaValerie KouskoffGeorges LacaudNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monika Stefanska Kiran Batta Rahima Patel Magdalena Florkowska Valerie Kouskoff Georges Lacaud Primitive erythrocytes are generated from hemogenic endothelial cells
description	Abstract Primitive erythroblasts are the first blood cells generated during embryonic hematopoiesis. Tracking their emergence both in vivo and in vitro has remained challenging due to the lack of specific cell surface markers. To selectively investigate primitive erythropoiesis, we have engineered a new transgenic embryonic stem (ES) cell line, where eGFP expression is driven by the regulatory sequences of the embryonic βH1 hemoglobin gene expressed specifically in primitive erythroid cells. Using this ES cell line, we observed that the first primitive erythroblasts are detected in vitro around day 1.5 of blast colony differentiation, within the cell population positive for the early hematopoietic progenitor marker CD41. Moreover, we establish that these eGFP+ cells emerge from a hemogenic endothelial cell population similarly to their definitive hematopoietic counterparts. We further generated a corresponding βH1-eGFP transgenic mouse model and demonstrated the presence of a primitive erythroid primed hemogenic endothelial cell population in the developing embryo. Taken together, our findings demonstrate that both in vivo and in vitro primitive erythrocytes are generated from hemogenic endothelial cells.
format	article
author	Monika Stefanska Kiran Batta Rahima Patel Magdalena Florkowska Valerie Kouskoff Georges Lacaud
author_facet	Monika Stefanska Kiran Batta Rahima Patel Magdalena Florkowska Valerie Kouskoff Georges Lacaud
author_sort	Monika Stefanska
title	Primitive erythrocytes are generated from hemogenic endothelial cells
title_short	Primitive erythrocytes are generated from hemogenic endothelial cells
title_full	Primitive erythrocytes are generated from hemogenic endothelial cells
title_fullStr	Primitive erythrocytes are generated from hemogenic endothelial cells
title_full_unstemmed	Primitive erythrocytes are generated from hemogenic endothelial cells
title_sort	primitive erythrocytes are generated from hemogenic endothelial cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c88a97e8e87c4720ba4b1208235e6341
work_keys_str_mv	AT monikastefanska primitiveerythrocytesaregeneratedfromhemogenicendothelialcells AT kiranbatta primitiveerythrocytesaregeneratedfromhemogenicendothelialcells AT rahimapatel primitiveerythrocytesaregeneratedfromhemogenicendothelialcells AT magdalenaflorkowska primitiveerythrocytesaregeneratedfromhemogenicendothelialcells AT valeriekouskoff primitiveerythrocytesaregeneratedfromhemogenicendothelialcells AT georgeslacaud primitiveerythrocytesaregeneratedfromhemogenicendothelialcells
_version_	1718394120007843840

Primitive erythrocytes are generated from hemogenic endothelial cells

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