Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii

Rosa roxburghii Tratt. is widely applied in food, cosmetics, and traditional medicine, and has been demonstrated to possess diverse bioactivities. Plant endophytic fungi are important microbial resources with great potential for application in many fields. They not only establish mutualistic symbios...

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Autores principales:	Hong Zhang, Tian-Peng Wei, Lin-Zhu Li, Ming-Yan Luo, Wei-Yu Jia, Yan Zeng, Yu-Lan Jiang, Guang-Can Tao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	molecular phylogeny alpha diversity lifestyle diversity intracellular metabolites extracellular metabolites Hypomontagnella monticulosa Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c88bd1d7363a4159b0401fe0ab59e1e5
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spelling	oai:doaj.org-article:c88bd1d7363a4159b0401fe0ab59e1e52021-12-01T13:54:43ZMultigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii1664-302X10.3389/fmicb.2021.755919https://doaj.org/article/c88bd1d7363a4159b0401fe0ab59e1e52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.755919/fullhttps://doaj.org/toc/1664-302XRosa roxburghii Tratt. is widely applied in food, cosmetics, and traditional medicine, and has been demonstrated to possess diverse bioactivities. Plant endophytic fungi are important microbial resources with great potential for application in many fields. They not only establish mutualistic symbiosis with host plants but also produce a variety of bioactive compounds. Therefore, in the present study, endophytic fungi were isolated from R. roxburghii, the diversity and antimicrobial activities were evaluated. As a result, 242 strains of endophytic Sordariomycetes were successfully isolated. Multigene phylogenetic analyses showed that these isolates included eight orders, 19 families, 33 genera. The dominant genera were Diaporthe (31.4%), Fusarium (14.4%), Chaetomium (7.9%), Dactylonectria (7.0%), Graphium (4.5%), Colletotrichum (4.1%), and Clonostachys (4.1%). For different tissues of R. roxburghii, alpha diversity analysis revealed that the diversity of fungal communities decreased in the order of root, fruit, stem, flower, leaf, and seed, and Clonostachys and Dactylonectria exhibited obvious tissue specificity. Meanwhile, functional annotation of 33 genera indicated that some fungi have multitrophic lifestyles combining endophytic, pathogenic, and saprophytic behavior. Additionally, antimicrobial activities of endophytic Sordariomycetes against Lasiodiplodia theobromae, Botryosphaeria dothidea, Colletotrichum capsici, Pyricularia oryzae, Rhizoctonia solani, Fusarium oxysporum, Pseudomonas syringae, Pantoea agglomerans, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa were screened. Dual culture test assays showed that there were 40 different endophytic species with strong inhibition of at least one or moderate inhibition of two or more against the 12 tested strains. The results from the filter paper diffusion method suggested that extracellular metabolites may be more advantageous than intracellular metabolites in the development of antimicrobial agents. Eleven isolates with good activities were screened. In particular, Hypomontagnella monticulosa HGUP194009 and Nigrospora sphaerica HGUP191020 have shown promise in both broad-spectrum and intensity. Finally, some fungi that commonly cause disease have been observed to have beneficial biological activities as endophytic fungi. In conclusion, this study showed the species composition, alpha diversity, and lifestyle diversity of endophytic Sordariomycetes from R. roxburghii and demonstrated these isolates are potential sources for exploring antimicrobial agents.Hong ZhangHong ZhangTian-Peng WeiLin-Zhu LiMing-Yan LuoWei-Yu JiaYan ZengYu-Lan JiangGuang-Can TaoGuang-Can TaoFrontiers Media S.A.articlemolecular phylogenyalpha diversitylifestyle diversityintracellular metabolitesextracellular metabolitesHypomontagnella monticulosaMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	molecular phylogeny alpha diversity lifestyle diversity intracellular metabolites extracellular metabolites Hypomontagnella monticulosa Microbiology QR1-502
spellingShingle	molecular phylogeny alpha diversity lifestyle diversity intracellular metabolites extracellular metabolites Hypomontagnella monticulosa Microbiology QR1-502 Hong Zhang Hong Zhang Tian-Peng Wei Lin-Zhu Li Ming-Yan Luo Wei-Yu Jia Yan Zeng Yu-Lan Jiang Guang-Can Tao Guang-Can Tao Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii
description	Rosa roxburghii Tratt. is widely applied in food, cosmetics, and traditional medicine, and has been demonstrated to possess diverse bioactivities. Plant endophytic fungi are important microbial resources with great potential for application in many fields. They not only establish mutualistic symbiosis with host plants but also produce a variety of bioactive compounds. Therefore, in the present study, endophytic fungi were isolated from R. roxburghii, the diversity and antimicrobial activities were evaluated. As a result, 242 strains of endophytic Sordariomycetes were successfully isolated. Multigene phylogenetic analyses showed that these isolates included eight orders, 19 families, 33 genera. The dominant genera were Diaporthe (31.4%), Fusarium (14.4%), Chaetomium (7.9%), Dactylonectria (7.0%), Graphium (4.5%), Colletotrichum (4.1%), and Clonostachys (4.1%). For different tissues of R. roxburghii, alpha diversity analysis revealed that the diversity of fungal communities decreased in the order of root, fruit, stem, flower, leaf, and seed, and Clonostachys and Dactylonectria exhibited obvious tissue specificity. Meanwhile, functional annotation of 33 genera indicated that some fungi have multitrophic lifestyles combining endophytic, pathogenic, and saprophytic behavior. Additionally, antimicrobial activities of endophytic Sordariomycetes against Lasiodiplodia theobromae, Botryosphaeria dothidea, Colletotrichum capsici, Pyricularia oryzae, Rhizoctonia solani, Fusarium oxysporum, Pseudomonas syringae, Pantoea agglomerans, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa were screened. Dual culture test assays showed that there were 40 different endophytic species with strong inhibition of at least one or moderate inhibition of two or more against the 12 tested strains. The results from the filter paper diffusion method suggested that extracellular metabolites may be more advantageous than intracellular metabolites in the development of antimicrobial agents. Eleven isolates with good activities were screened. In particular, Hypomontagnella monticulosa HGUP194009 and Nigrospora sphaerica HGUP191020 have shown promise in both broad-spectrum and intensity. Finally, some fungi that commonly cause disease have been observed to have beneficial biological activities as endophytic fungi. In conclusion, this study showed the species composition, alpha diversity, and lifestyle diversity of endophytic Sordariomycetes from R. roxburghii and demonstrated these isolates are potential sources for exploring antimicrobial agents.
format	article
author	Hong Zhang Hong Zhang Tian-Peng Wei Lin-Zhu Li Ming-Yan Luo Wei-Yu Jia Yan Zeng Yu-Lan Jiang Guang-Can Tao Guang-Can Tao
author_facet	Hong Zhang Hong Zhang Tian-Peng Wei Lin-Zhu Li Ming-Yan Luo Wei-Yu Jia Yan Zeng Yu-Lan Jiang Guang-Can Tao Guang-Can Tao
author_sort	Hong Zhang
title	Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii
title_short	Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii
title_full	Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii
title_fullStr	Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii
title_full_unstemmed	Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii
title_sort	multigene phylogeny, diversity and antimicrobial potential of endophytic sordariomycetes from rosa roxburghii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c88bd1d7363a4159b0401fe0ab59e1e5
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Multigene Phylogeny, Diversity and Antimicrobial Potential of Endophytic Sordariomycetes From Rosa roxburghii

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