A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR

Abstract Natural transformation is used by bacteria to take up DNA from their surroundings and incorporate it into their genomes. Streptococci do so during a transient period of competence, triggered by pheromones that they produce, secrete and sense under conditions influenced by the environment. I...

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Autores principales:	Rabia Khan, Roger Junges, Heidi A. Åmdal, Tsute Chen, Donald A. Morrison, Fernanda C. Petersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c89341c654fd4d27be4e4697768736e9
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spelling	oai:doaj.org-article:c89341c654fd4d27be4e4697768736e92021-12-02T12:32:30ZA positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR10.1038/s41598-017-04768-52045-2322https://doaj.org/article/c89341c654fd4d27be4e4697768736e92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04768-5https://doaj.org/toc/2045-2322Abstract Natural transformation is used by bacteria to take up DNA from their surroundings and incorporate it into their genomes. Streptococci do so during a transient period of competence, triggered by pheromones that they produce, secrete and sense under conditions influenced by the environment. In Streptococcus mutans, Streptococcus suis, and species of the bovis, salivarius and pyogenic groups of streptococci, the pheromone XIP is sensed by the intra-cellular regulator ComR, that in turn activates the transcription of comS, encoding the XIP precursor, and of sigX, encoding the only known alternative sigma factor in streptococci. Although induction of comR during competence has been known for more than fifteen years, the mechanism regulating its expression remains unidentified. By a combination of directional RNA-sequencing, optimal competence conditions, stepwise deletions and marker-less genome editing, we found that SigX is the missing link in overproduction of ComR. In the absence of comR induction, both sigX expression and transformation were significantly reduced. Placing comR and comS transcripts under the control of different regulators so as to form two interlocked positive feedback circuits may enable S. mutans to fine-tune the kinetics and magnitude of the competence response according to their need.Rabia KhanRoger JungesHeidi A. ÅmdalTsute ChenDonald A. MorrisonFernanda C. PetersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rabia Khan Roger Junges Heidi A. Åmdal Tsute Chen Donald A. Morrison Fernanda C. Petersen A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR
description	Abstract Natural transformation is used by bacteria to take up DNA from their surroundings and incorporate it into their genomes. Streptococci do so during a transient period of competence, triggered by pheromones that they produce, secrete and sense under conditions influenced by the environment. In Streptococcus mutans, Streptococcus suis, and species of the bovis, salivarius and pyogenic groups of streptococci, the pheromone XIP is sensed by the intra-cellular regulator ComR, that in turn activates the transcription of comS, encoding the XIP precursor, and of sigX, encoding the only known alternative sigma factor in streptococci. Although induction of comR during competence has been known for more than fifteen years, the mechanism regulating its expression remains unidentified. By a combination of directional RNA-sequencing, optimal competence conditions, stepwise deletions and marker-less genome editing, we found that SigX is the missing link in overproduction of ComR. In the absence of comR induction, both sigX expression and transformation were significantly reduced. Placing comR and comS transcripts under the control of different regulators so as to form two interlocked positive feedback circuits may enable S. mutans to fine-tune the kinetics and magnitude of the competence response according to their need.
format	article
author	Rabia Khan Roger Junges Heidi A. Åmdal Tsute Chen Donald A. Morrison Fernanda C. Petersen
author_facet	Rabia Khan Roger Junges Heidi A. Åmdal Tsute Chen Donald A. Morrison Fernanda C. Petersen
author_sort	Rabia Khan
title	A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR
title_short	A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR
title_full	A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR
title_fullStr	A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR
title_full_unstemmed	A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR
title_sort	positive feedback loop mediated by sigma x enhances expression of the streptococcal regulator comr
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c89341c654fd4d27be4e4697768736e9
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A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR

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