Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR.
Angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) are subtypes of T-cell lymphoma. Due to low tumor cell content and substantial reactive cell infiltration, these lymphomas are sometimes mistaken for other types of lymphomas or even non-neop...
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Public Library of Science (PLoS)
2014
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oai:doaj.org-article:c89b8a08bdaa4801ad647804d274c3222021-11-25T05:56:52ZDetection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR.1932-620310.1371/journal.pone.0109714https://doaj.org/article/c89b8a08bdaa4801ad647804d274c3222014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109714https://doaj.org/toc/1932-6203Angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) are subtypes of T-cell lymphoma. Due to low tumor cell content and substantial reactive cell infiltration, these lymphomas are sometimes mistaken for other types of lymphomas or even non-neoplastic diseases. In addition, a significant proportion of PTCL-NOS cases reportedly exhibit features of AITL (AITL-like PTCL-NOS). Thus disagreement is common in distinguishing between AITL and PTCL-NOS. Using whole-exome and subsequent targeted sequencing, we recently identified G17V RHOA mutations in 60-70% of AITL and AITL-like PTCL-NOS cases but not in other hematologic cancers, including other T-cell malignancies. Here, we establish a sensitive detection method for the G17V RHOA mutation using a quantitative allele-specific polymerase chain reaction (qAS-PCR) assay. Mutated allele frequencies deduced from this approach were highly correlated with those determined by deep sequencing. This method could serve as a novel diagnostic tool for 60-70% of AITL and AITL-like PTCL-NOS.Rie Nakamoto-MatsubaraMamiko Sakata-YanagimotoTerukazu EnamiKenichi YoshidaShintaro YanagimotoYusuke ShiozawaTohru NanmokuKaishi SatomiHideharu MutoNaoshi ObaraTakayasu KatoNaoki KuritaYasuhisa YokoyamaKoji IzutsuYasunori OtaMasashi SanadaSeiichi ShimizuTakuya KomenoYuji SatoTakayoshi ItoIssay KitabayashiKengo TakeuchiNaoya NakamuraSeishi OgawaShigeru ChibaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109714 (2014) |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Rie Nakamoto-Matsubara Mamiko Sakata-Yanagimoto Terukazu Enami Kenichi Yoshida Shintaro Yanagimoto Yusuke Shiozawa Tohru Nanmoku Kaishi Satomi Hideharu Muto Naoshi Obara Takayasu Kato Naoki Kurita Yasuhisa Yokoyama Koji Izutsu Yasunori Ota Masashi Sanada Seiichi Shimizu Takuya Komeno Yuji Sato Takayoshi Ito Issay Kitabayashi Kengo Takeuchi Naoya Nakamura Seishi Ogawa Shigeru Chiba Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR. |
| description |
Angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) are subtypes of T-cell lymphoma. Due to low tumor cell content and substantial reactive cell infiltration, these lymphomas are sometimes mistaken for other types of lymphomas or even non-neoplastic diseases. In addition, a significant proportion of PTCL-NOS cases reportedly exhibit features of AITL (AITL-like PTCL-NOS). Thus disagreement is common in distinguishing between AITL and PTCL-NOS. Using whole-exome and subsequent targeted sequencing, we recently identified G17V RHOA mutations in 60-70% of AITL and AITL-like PTCL-NOS cases but not in other hematologic cancers, including other T-cell malignancies. Here, we establish a sensitive detection method for the G17V RHOA mutation using a quantitative allele-specific polymerase chain reaction (qAS-PCR) assay. Mutated allele frequencies deduced from this approach were highly correlated with those determined by deep sequencing. This method could serve as a novel diagnostic tool for 60-70% of AITL and AITL-like PTCL-NOS. |
| format |
article |
| author |
Rie Nakamoto-Matsubara Mamiko Sakata-Yanagimoto Terukazu Enami Kenichi Yoshida Shintaro Yanagimoto Yusuke Shiozawa Tohru Nanmoku Kaishi Satomi Hideharu Muto Naoshi Obara Takayasu Kato Naoki Kurita Yasuhisa Yokoyama Koji Izutsu Yasunori Ota Masashi Sanada Seiichi Shimizu Takuya Komeno Yuji Sato Takayoshi Ito Issay Kitabayashi Kengo Takeuchi Naoya Nakamura Seishi Ogawa Shigeru Chiba |
| author_facet |
Rie Nakamoto-Matsubara Mamiko Sakata-Yanagimoto Terukazu Enami Kenichi Yoshida Shintaro Yanagimoto Yusuke Shiozawa Tohru Nanmoku Kaishi Satomi Hideharu Muto Naoshi Obara Takayasu Kato Naoki Kurita Yasuhisa Yokoyama Koji Izutsu Yasunori Ota Masashi Sanada Seiichi Shimizu Takuya Komeno Yuji Sato Takayoshi Ito Issay Kitabayashi Kengo Takeuchi Naoya Nakamura Seishi Ogawa Shigeru Chiba |
| author_sort |
Rie Nakamoto-Matsubara |
| title |
Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR. |
| title_short |
Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR. |
| title_full |
Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR. |
| title_fullStr |
Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR. |
| title_full_unstemmed |
Detection of the G17V RHOA mutation in angioimmunoblastic T-cell lymphoma and related lymphomas using quantitative allele-specific PCR. |
| title_sort |
detection of the g17v rhoa mutation in angioimmunoblastic t-cell lymphoma and related lymphomas using quantitative allele-specific pcr. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/c89b8a08bdaa4801ad647804d274c322 |
| work_keys_str_mv |
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| _version_ |
1718414367361335296 |