Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins

To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification....

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Autores principales:	Fei Jin, Cheng Shen, Yao Wang, Mengqi Wang, Minxuan Sun, Motoyuki Hattori
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c89bae6b37bc44658ad0cfb2e371e704
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spelling	oai:doaj.org-article:c89bae6b37bc44658ad0cfb2e371e7042021-12-02T16:30:46ZFluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins10.1038/s42003-021-01891-y2399-3642https://doaj.org/article/c89bae6b37bc44658ad0cfb2e371e7042021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01891-yhttps://doaj.org/toc/2399-3642To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification. It holds promise as a powerful tool for screening of membrane proteins for functional and structural studies.Fei JinCheng ShenYao WangMengqi WangMinxuan SunMotoyuki HattoriNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Fei Jin Cheng Shen Yao Wang Mengqi Wang Minxuan Sun Motoyuki Hattori Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
description	To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification. It holds promise as a powerful tool for screening of membrane proteins for functional and structural studies.
format	article
author	Fei Jin Cheng Shen Yao Wang Mengqi Wang Minxuan Sun Motoyuki Hattori
author_facet	Fei Jin Cheng Shen Yao Wang Mengqi Wang Minxuan Sun Motoyuki Hattori
author_sort	Fei Jin
title	Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
title_short	Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
title_full	Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
title_fullStr	Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
title_full_unstemmed	Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
title_sort	fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c89bae6b37bc44658ad0cfb2e371e704
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Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins

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