Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification....
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/c89bae6b37bc44658ad0cfb2e371e704 |
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