A 3D adult zebrafish brain atlas (AZBA) for the digital age

Zebrafish have made significant contributions to our understanding of the vertebrate brain and the neural basis of behavior, earning a place as one of the most widely used model organisms in neuroscience. Their appeal arises from the marriage of low cost, early life transparency, and ease of genetic...

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Autores principales:	Justin W Kenney, Patrick E Steadman, Olivia Young, Meng Ting Shi, Maris Polanco, Saba Dubaishi, Kristopher Covert, Thomas Mueller, Paul W Frankland
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	brain atlas light-sheet microscopy adult zebrafish image registration tissue clearing iDISCO Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c89d4a3206304ffc9298f61e08621f8d
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spelling	oai:doaj.org-article:c89d4a3206304ffc9298f61e08621f8d2021-12-02T15:13:54ZA 3D adult zebrafish brain atlas (AZBA) for the digital age10.7554/eLife.699882050-084Xe69988https://doaj.org/article/c89d4a3206304ffc9298f61e08621f8d2021-11-01T00:00:00Zhttps://elifesciences.org/articles/69988https://doaj.org/toc/2050-084XZebrafish have made significant contributions to our understanding of the vertebrate brain and the neural basis of behavior, earning a place as one of the most widely used model organisms in neuroscience. Their appeal arises from the marriage of low cost, early life transparency, and ease of genetic manipulation with a behavioral repertoire that becomes more sophisticated as animals transition from larvae to adults. To further enhance the use of adult zebrafish, we created the first fully segmented three-dimensional digital adult zebrafish brain atlas (AZBA). AZBA was built by combining tissue clearing, light-sheet fluorescence microscopy, and three-dimensional image registration of nuclear and antibody stains. These images were used to guide segmentation of the atlas into over 200 neuroanatomical regions comprising the entirety of the adult zebrafish brain. As an open source, online (azba.wayne.edu), updatable digital resource, AZBA will significantly enhance the use of adult zebrafish in furthering our understanding of vertebrate brain function in both health and disease.Justin W KenneyPatrick E SteadmanOlivia YoungMeng Ting ShiMaris PolancoSaba DubaishiKristopher CovertThomas MuellerPaul W FranklandeLife Sciences Publications Ltdarticlebrain atlaslight-sheet microscopyadult zebrafishimage registrationtissue clearingiDISCOMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	brain atlas light-sheet microscopy adult zebrafish image registration tissue clearing iDISCO Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	brain atlas light-sheet microscopy adult zebrafish image registration tissue clearing iDISCO Medicine R Science Q Biology (General) QH301-705.5 Justin W Kenney Patrick E Steadman Olivia Young Meng Ting Shi Maris Polanco Saba Dubaishi Kristopher Covert Thomas Mueller Paul W Frankland A 3D adult zebrafish brain atlas (AZBA) for the digital age
description	Zebrafish have made significant contributions to our understanding of the vertebrate brain and the neural basis of behavior, earning a place as one of the most widely used model organisms in neuroscience. Their appeal arises from the marriage of low cost, early life transparency, and ease of genetic manipulation with a behavioral repertoire that becomes more sophisticated as animals transition from larvae to adults. To further enhance the use of adult zebrafish, we created the first fully segmented three-dimensional digital adult zebrafish brain atlas (AZBA). AZBA was built by combining tissue clearing, light-sheet fluorescence microscopy, and three-dimensional image registration of nuclear and antibody stains. These images were used to guide segmentation of the atlas into over 200 neuroanatomical regions comprising the entirety of the adult zebrafish brain. As an open source, online (azba.wayne.edu), updatable digital resource, AZBA will significantly enhance the use of adult zebrafish in furthering our understanding of vertebrate brain function in both health and disease.
format	article
author	Justin W Kenney Patrick E Steadman Olivia Young Meng Ting Shi Maris Polanco Saba Dubaishi Kristopher Covert Thomas Mueller Paul W Frankland
author_facet	Justin W Kenney Patrick E Steadman Olivia Young Meng Ting Shi Maris Polanco Saba Dubaishi Kristopher Covert Thomas Mueller Paul W Frankland
author_sort	Justin W Kenney
title	A 3D adult zebrafish brain atlas (AZBA) for the digital age
title_short	A 3D adult zebrafish brain atlas (AZBA) for the digital age
title_full	A 3D adult zebrafish brain atlas (AZBA) for the digital age
title_fullStr	A 3D adult zebrafish brain atlas (AZBA) for the digital age
title_full_unstemmed	A 3D adult zebrafish brain atlas (AZBA) for the digital age
title_sort	3d adult zebrafish brain atlas (azba) for the digital age
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/c89d4a3206304ffc9298f61e08621f8d
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A 3D adult zebrafish brain atlas (AZBA) for the digital age

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