Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway

Inflammatory microenvironment after transplantation affects the proliferation and causes senescence of adipose-derived mesenchymal stem cells (hADMSCs) thus compromising their clinical efficacy. Priming stem cells with herbal extracts is considered very promising to improve their viability in the in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hamzah Khawaja, Numan Fazal, Faiza Yaqub, Muhammad Rauf Ahmad, Muzaffar Hanif, Muhammad Amin Yousaf, Noreen Latief
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c89d8c8edd814be7bce35876fa4ddeb9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c89d8c8edd814be7bce35876fa4ddeb9
record_format	dspace
spelling	oai:doaj.org-article:c89d8c8edd814be7bce35876fa4ddeb92021-11-04T06:07:13ZProtective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway1932-6203https://doaj.org/article/c89d8c8edd814be7bce35876fa4ddeb92021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535185/?tool=EBIhttps://doaj.org/toc/1932-6203Inflammatory microenvironment after transplantation affects the proliferation and causes senescence of adipose-derived mesenchymal stem cells (hADMSCs) thus compromising their clinical efficacy. Priming stem cells with herbal extracts is considered very promising to improve their viability in the inflammatory milieu. Aesculus indica (A. indica) is used to treat many inflammatory diseases in Asia for decades. Herein, we explored the protective role of A. indica extract on human adipose-derived Mesenchymal Stem Cells (hADMSCs) against Monosodium Iodoacetate (MIA) induced stress in vitro. A. indica ameliorated the injury as depicted by significantly enhanced proliferation, viability, improved cell migration and superoxide dismutase activity. Furthermore, reduced lactate dehydrogenase activity, reactive oxygen species release, senescent and apoptotic cells were detected in A. indica primed hADMSCs. Downregulation of NF-κB pathway and associated inflammatory genes, NF-κB p65/RelA and p50/NF-κB 1, Interleukin 6 (IL-6), Interleukin 1 (IL-1β), Tumor necrosis factor alpha (TNF-α) and matrix metalloproteinase 13 (MMP-13) were observed in A. indica primed hADMSCs as compared to stressed hADMSCs. Complementary to gene expression, A. indica priming reduced the release of transcription factor p65, inhibitory-κB kinase (IKK) α and β, IL-1β and TNF-α proteins expression. Our data elucidates that A. indica extract preconditioning rescued hADMSCs against oxidative stress and improved their therapeutic potential by relieving inflammation through regulation of NF-κB pathway.Hamzah KhawajaNuman FazalFaiza YaqubMuhammad Rauf AhmadMuzaffar HanifMuhammad Amin YousafNoreen LatiefPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamzah Khawaja Numan Fazal Faiza Yaqub Muhammad Rauf Ahmad Muzaffar Hanif Muhammad Amin Yousaf Noreen Latief Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway
description	Inflammatory microenvironment after transplantation affects the proliferation and causes senescence of adipose-derived mesenchymal stem cells (hADMSCs) thus compromising their clinical efficacy. Priming stem cells with herbal extracts is considered very promising to improve their viability in the inflammatory milieu. Aesculus indica (A. indica) is used to treat many inflammatory diseases in Asia for decades. Herein, we explored the protective role of A. indica extract on human adipose-derived Mesenchymal Stem Cells (hADMSCs) against Monosodium Iodoacetate (MIA) induced stress in vitro. A. indica ameliorated the injury as depicted by significantly enhanced proliferation, viability, improved cell migration and superoxide dismutase activity. Furthermore, reduced lactate dehydrogenase activity, reactive oxygen species release, senescent and apoptotic cells were detected in A. indica primed hADMSCs. Downregulation of NF-κB pathway and associated inflammatory genes, NF-κB p65/RelA and p50/NF-κB 1, Interleukin 6 (IL-6), Interleukin 1 (IL-1β), Tumor necrosis factor alpha (TNF-α) and matrix metalloproteinase 13 (MMP-13) were observed in A. indica primed hADMSCs as compared to stressed hADMSCs. Complementary to gene expression, A. indica priming reduced the release of transcription factor p65, inhibitory-κB kinase (IKK) α and β, IL-1β and TNF-α proteins expression. Our data elucidates that A. indica extract preconditioning rescued hADMSCs against oxidative stress and improved their therapeutic potential by relieving inflammation through regulation of NF-κB pathway.
format	article
author	Hamzah Khawaja Numan Fazal Faiza Yaqub Muhammad Rauf Ahmad Muzaffar Hanif Muhammad Amin Yousaf Noreen Latief
author_facet	Hamzah Khawaja Numan Fazal Faiza Yaqub Muhammad Rauf Ahmad Muzaffar Hanif Muhammad Amin Yousaf Noreen Latief
author_sort	Hamzah Khawaja
title	Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway
title_short	Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway
title_full	Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway
title_fullStr	Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway
title_full_unstemmed	Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway
title_sort	protective and proliferative effect of aesculus indica extract on stressed human adipose stem cells via downregulation of nf-κb pathway
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c89d8c8edd814be7bce35876fa4ddeb9
work_keys_str_mv	AT hamzahkhawaja protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway AT numanfazal protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway AT faizayaqub protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway AT muhammadraufahmad protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway AT muzaffarhanif protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway AT muhammadaminyousaf protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway AT noreenlatief protectiveandproliferativeeffectofaesculusindicaextractonstressedhumanadiposestemcellsviadownregulationofnfkbpathway
_version_	1718445176051990528

Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway

Ejemplares similares