Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.

Naturally-derived proteins or peptides are promising biopolymers for tissue engineering applications owing to their health-promoting activity. Herein, we extracted proteins (~90%) from two-spotted cricket (Gryllus bimaculatus) and evaluated their osteoinductive potential in human bone marrow-derived...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Keya Ganguly, Sayan Deb Dutta, Min-Soo Jeong, Dinesh K Patel, Seong-Jun Cho, Ki-Taek Lim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c89fc4d9d4ef4dffb5d47ba13e6ca1d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c89fc4d9d4ef4dffb5d47ba13e6ca1d3
record_format	dspace
spelling	oai:doaj.org-article:c89fc4d9d4ef4dffb5d47ba13e6ca1d32021-12-02T20:05:24ZNaturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.1932-620310.1371/journal.pone.0249291https://doaj.org/article/c89fc4d9d4ef4dffb5d47ba13e6ca1d32021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249291https://doaj.org/toc/1932-6203Naturally-derived proteins or peptides are promising biopolymers for tissue engineering applications owing to their health-promoting activity. Herein, we extracted proteins (~90%) from two-spotted cricket (Gryllus bimaculatus) and evaluated their osteoinductive potential in human bone marrow-derived mesenchymal stem cells (hBMSCs) under in vitro conditions. The extracted protein isolate was analyzed for the amino acid composition and the mass distribution of the constituent peptide fraction. Fourier transform infrared (FTIR) spectroscopy was used to determine the presence of biologically significant functional groups. The cricket protein isolate (CPI) exhibited characteristic protein peaks in the FTIR spectrum. Notably, an enhanced cell viability was observed in the presence of the extracted proteins, showing their biocompatibility. The CPI also exhibited antioxidant properties in a concentration-dependent manner. More significant mineralization was observed in the CPI-treated cells than in the control, suggesting their osteoinductive potential. The upregulation of the osteogenic marker genes (Runx2, ALP, OCN, and BSP) in CPI treated media compared with the control supports their osteoinductive nature. Therefore, cricket-derived protein isolates could be used as functional protein isolate for tissue engineering applications, especially for bone regeneration.Keya GangulySayan Deb DuttaMin-Soo JeongDinesh K PatelSeong-Jun ChoKi-Taek LimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0249291 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keya Ganguly Sayan Deb Dutta Min-Soo Jeong Dinesh K Patel Seong-Jun Cho Ki-Taek Lim Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.
description	Naturally-derived proteins or peptides are promising biopolymers for tissue engineering applications owing to their health-promoting activity. Herein, we extracted proteins (~90%) from two-spotted cricket (Gryllus bimaculatus) and evaluated their osteoinductive potential in human bone marrow-derived mesenchymal stem cells (hBMSCs) under in vitro conditions. The extracted protein isolate was analyzed for the amino acid composition and the mass distribution of the constituent peptide fraction. Fourier transform infrared (FTIR) spectroscopy was used to determine the presence of biologically significant functional groups. The cricket protein isolate (CPI) exhibited characteristic protein peaks in the FTIR spectrum. Notably, an enhanced cell viability was observed in the presence of the extracted proteins, showing their biocompatibility. The CPI also exhibited antioxidant properties in a concentration-dependent manner. More significant mineralization was observed in the CPI-treated cells than in the control, suggesting their osteoinductive potential. The upregulation of the osteogenic marker genes (Runx2, ALP, OCN, and BSP) in CPI treated media compared with the control supports their osteoinductive nature. Therefore, cricket-derived protein isolates could be used as functional protein isolate for tissue engineering applications, especially for bone regeneration.
format	article
author	Keya Ganguly Sayan Deb Dutta Min-Soo Jeong Dinesh K Patel Seong-Jun Cho Ki-Taek Lim
author_facet	Keya Ganguly Sayan Deb Dutta Min-Soo Jeong Dinesh K Patel Seong-Jun Cho Ki-Taek Lim
author_sort	Keya Ganguly
title	Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.
title_short	Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.
title_full	Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.
title_fullStr	Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.
title_full_unstemmed	Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.
title_sort	naturally-derived protein extract from gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hbmscs.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c89fc4d9d4ef4dffb5d47ba13e6ca1d3
work_keys_str_mv	AT keyaganguly naturallyderivedproteinextractfromgryllusbimaculatusimprovesantioxidantpropertiesandpromotesosteogenicdifferentiationofhbmscs AT sayandebdutta naturallyderivedproteinextractfromgryllusbimaculatusimprovesantioxidantpropertiesandpromotesosteogenicdifferentiationofhbmscs AT minsoojeong naturallyderivedproteinextractfromgryllusbimaculatusimprovesantioxidantpropertiesandpromotesosteogenicdifferentiationofhbmscs AT dineshkpatel naturallyderivedproteinextractfromgryllusbimaculatusimprovesantioxidantpropertiesandpromotesosteogenicdifferentiationofhbmscs AT seongjuncho naturallyderivedproteinextractfromgryllusbimaculatusimprovesantioxidantpropertiesandpromotesosteogenicdifferentiationofhbmscs AT kitaeklim naturallyderivedproteinextractfromgryllusbimaculatusimprovesantioxidantpropertiesandpromotesosteogenicdifferentiationofhbmscs
_version_	1718375506736316416

Naturally-derived protein extract from Gryllus bimaculatus improves antioxidant properties and promotes osteogenic differentiation of hBMSCs.

Ejemplares similares