Chemical clearing and dehydration of GFP expressing mouse brains.
Generally, chemical tissue clearing is performed by a solution consisting of two parts benzyl benzoate and one part benzyl alcohol. However, prolonged exposure to this mixture markedly reduces the fluorescence of GFP expressing specimens, so that one has to compromise between clearing quality and fl...
Guardado en:
Autores principales: | Klaus Becker, Nina Jährling, Saiedeh Saghafi, Reto Weiler, Hans-Ulrich Dodt |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2012
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Materias: | |
Acceso en línea: | https://doaj.org/article/c8a46938fac3465189af428831afe271 |
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