Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations

Abstract This work reports on an effort to decipher the alignment of brain microvasculature endothelial cells to physical constrains generated via adhesion control on hydrogel surfaces and explore the corresponding responses upon glucose level variations emulating the hypo- and hyperglycaemic effect...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ana María Porras Hernández, Laurent Barbe, Hannah Pohlit, Maria Tenje, Maria Antfolk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c8aa27fd06a24de6bfbad4a9ee2e8843
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c8aa27fd06a24de6bfbad4a9ee2e8843
record_format	dspace
spelling	oai:doaj.org-article:c8aa27fd06a24de6bfbad4a9ee2e88432021-12-02T17:13:17ZBrain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations10.1038/s41598-021-99136-92045-2322https://doaj.org/article/c8aa27fd06a24de6bfbad4a9ee2e88432021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99136-9https://doaj.org/toc/2045-2322Abstract This work reports on an effort to decipher the alignment of brain microvasculature endothelial cells to physical constrains generated via adhesion control on hydrogel surfaces and explore the corresponding responses upon glucose level variations emulating the hypo- and hyperglycaemic effects in diabetes. We prepared hydrogels of hyaluronic acid a natural biomaterial that does not naturally support endothelial cell adhesion, and specifically functionalised RGD peptides into lines using UV-mediated linkage. The width of the lines was varied from 10 to 100 µm. We evaluated cell alignment by measuring the nuclei, cell, and F-actin orientations, and the nuclei and cell eccentricity via immunofluorescent staining and image analysis. We found that the brain microvascular endothelial cells aligned and elongated to these physical constraints for all line widths. In addition, we also observed that varying the cell medium glucose levels affected the cell alignment along the patterns. We believe our results may provide a platform for further studies on the impact of altered glucose levels in cardiovascular disease.Ana María Porras HernándezLaurent BarbeHannah PohlitMaria TenjeMaria AntfolkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana María Porras Hernández Laurent Barbe Hannah Pohlit Maria Tenje Maria Antfolk Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
description	Abstract This work reports on an effort to decipher the alignment of brain microvasculature endothelial cells to physical constrains generated via adhesion control on hydrogel surfaces and explore the corresponding responses upon glucose level variations emulating the hypo- and hyperglycaemic effects in diabetes. We prepared hydrogels of hyaluronic acid a natural biomaterial that does not naturally support endothelial cell adhesion, and specifically functionalised RGD peptides into lines using UV-mediated linkage. The width of the lines was varied from 10 to 100 µm. We evaluated cell alignment by measuring the nuclei, cell, and F-actin orientations, and the nuclei and cell eccentricity via immunofluorescent staining and image analysis. We found that the brain microvascular endothelial cells aligned and elongated to these physical constraints for all line widths. In addition, we also observed that varying the cell medium glucose levels affected the cell alignment along the patterns. We believe our results may provide a platform for further studies on the impact of altered glucose levels in cardiovascular disease.
format	article
author	Ana María Porras Hernández Laurent Barbe Hannah Pohlit Maria Tenje Maria Antfolk
author_facet	Ana María Porras Hernández Laurent Barbe Hannah Pohlit Maria Tenje Maria Antfolk
author_sort	Ana María Porras Hernández
title	Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
title_short	Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
title_full	Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
title_fullStr	Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
title_full_unstemmed	Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
title_sort	brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c8aa27fd06a24de6bfbad4a9ee2e8843
work_keys_str_mv	AT anamariaporrashernandez brainmicrovasculatureendothelialcellorientationonmicropatternedhydrogelsisaffectedbyglucoselevelvariations AT laurentbarbe brainmicrovasculatureendothelialcellorientationonmicropatternedhydrogelsisaffectedbyglucoselevelvariations AT hannahpohlit brainmicrovasculatureendothelialcellorientationonmicropatternedhydrogelsisaffectedbyglucoselevelvariations AT mariatenje brainmicrovasculatureendothelialcellorientationonmicropatternedhydrogelsisaffectedbyglucoselevelvariations AT mariaantfolk brainmicrovasculatureendothelialcellorientationonmicropatternedhydrogelsisaffectedbyglucoselevelvariations
_version_	1718381405744922624

Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations

Ejemplares similares