The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.

The Orb CPEB protein regulates translation of localized mRNAs in Drosophila ovaries. While there are multiple hypo- and hyperphosphorylated Orb isoforms in wild type ovaries, most are missing in orb(F303), which has an amino acid substitution in a buried region of the second RRM domain. Using a prot...

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Autores principales:	Li Chin Wong, Alexandre Costa, Ian McLeod, Ali Sarkeshik, John Yates, Saw Kyin, David Perlman, Paul Schedl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c8abd26a2aad41bba722dd926f5cad68
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spelling	oai:doaj.org-article:c8abd26a2aad41bba722dd926f5cad682021-11-04T06:08:19ZThe functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.1932-620310.1371/journal.pone.0024355https://doaj.org/article/c8abd26a2aad41bba722dd926f5cad682011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949709/?tool=EBIhttps://doaj.org/toc/1932-6203The Orb CPEB protein regulates translation of localized mRNAs in Drosophila ovaries. While there are multiple hypo- and hyperphosphorylated Orb isoforms in wild type ovaries, most are missing in orb(F303), which has an amino acid substitution in a buried region of the second RRM domain. Using a proteomics approach we identified a candidate Orb kinase, Casein Kinase 2 (CK2). In addition to being associated with Orb in vivo, we show that ck2 is required for orb functioning in gurken signaling and in the autoregulation of orb mRNA localization and translation. Supporting a role for ck2 in Orb phosphorylation, we find that the phosphorylation pattern is altered when ck2 activity is partially compromised. Finally, we show that the Orb hypophosphorylated isoforms are in slowly sedimenting complexes that contain the translational repressor Bruno, while the hyperphosphorylated isoforms assemble into large complexes that co-sediment with polysomes and contain the Wisp poly(A) polymerase.Li Chin WongAlexandre CostaIan McLeodAli SarkeshikJohn YatesSaw KyinDavid PerlmanPaul SchedlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24355 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Chin Wong Alexandre Costa Ian McLeod Ali Sarkeshik John Yates Saw Kyin David Perlman Paul Schedl The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
description	The Orb CPEB protein regulates translation of localized mRNAs in Drosophila ovaries. While there are multiple hypo- and hyperphosphorylated Orb isoforms in wild type ovaries, most are missing in orb(F303), which has an amino acid substitution in a buried region of the second RRM domain. Using a proteomics approach we identified a candidate Orb kinase, Casein Kinase 2 (CK2). In addition to being associated with Orb in vivo, we show that ck2 is required for orb functioning in gurken signaling and in the autoregulation of orb mRNA localization and translation. Supporting a role for ck2 in Orb phosphorylation, we find that the phosphorylation pattern is altered when ck2 activity is partially compromised. Finally, we show that the Orb hypophosphorylated isoforms are in slowly sedimenting complexes that contain the translational repressor Bruno, while the hyperphosphorylated isoforms assemble into large complexes that co-sediment with polysomes and contain the Wisp poly(A) polymerase.
format	article
author	Li Chin Wong Alexandre Costa Ian McLeod Ali Sarkeshik John Yates Saw Kyin David Perlman Paul Schedl
author_facet	Li Chin Wong Alexandre Costa Ian McLeod Ali Sarkeshik John Yates Saw Kyin David Perlman Paul Schedl
author_sort	Li Chin Wong
title	The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
title_short	The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
title_full	The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
title_fullStr	The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
title_full_unstemmed	The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.
title_sort	functioning of the drosophila cpeb protein orb is regulated by phosphorylation and requires casein kinase 2 activity.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c8abd26a2aad41bba722dd926f5cad68
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The functioning of the Drosophila CPEB protein Orb is regulated by phosphorylation and requires casein kinase 2 activity.

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