Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish

White adipose tissue hyperplasia has been shown to be crucial for handling excess energy in healthy ways. Though adipogenesis mechanisms have been underscored in vitro, we lack information on how tissue and systemic factors influence the differentiation of new adipocytes. While this could be studied...

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Autores principales:	Paola Lepanto, Florencia Levin-Ferreyra, Uriel Koziol, Leonel Malacrida, José L. Badano
Formato:	article
Lenguaje:	EN
Publicado:	The Company of Biologists 2021
Materias:	zebrafish adipocyte nile red blood vessels Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c8bbec3156d344d897d0cd5129adf7d2
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spelling	oai:doaj.org-article:c8bbec3156d344d897d0cd5129adf7d22021-11-28T16:01:13ZInsights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish2046-639010.1242/bio.058734https://doaj.org/article/c8bbec3156d344d897d0cd5129adf7d22021-09-01T00:00:00Zhttp://bio.biologists.org/content/10/9/bio058734https://doaj.org/toc/2046-6390White adipose tissue hyperplasia has been shown to be crucial for handling excess energy in healthy ways. Though adipogenesis mechanisms have been underscored in vitro, we lack information on how tissue and systemic factors influence the differentiation of new adipocytes. While this could be studied in zebrafish, adipocyte identification currently relies on neutral lipid labeling, thus precluding access to cells in early stages of differentiation. Here we report the generation and analysis of a zebrafish line with the transgene fabp4a(-2.7):EGFPcaax. In vivo confocal microscopy of the pancreatic and abdominal visceral depots of transgenic larvae, revealed the presence of labeled mature adipocytes as well as immature cells in earlier stages of differentiation. Through co-labeling for blood vessels, we observed a close interaction of differentiating adipocytes with endothelial cells through cell protrusions. Finally, we implemented hyperspectral imaging and spectral phasor analysis in Nile Red-labeled transgenic larvae and revealed the lipid metabolic transition towards neutral lipid accumulation of differentiating adipocytes. Altogether our work presents the characterization of a novel adipocyte-specific label in zebrafish and uncovers previously unknown aspects of in vivo adipogenesis. This article has an associated First Person interview with the first author of the paper.Paola LepantoFlorencia Levin-FerreyraUriel KoziolLeonel MalacridaJosé L. BadanoThe Company of Biologistsarticlezebrafishadipocytenile redblood vesselsScienceQBiology (General)QH301-705.5ENBiology Open, Vol 10, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish adipocyte nile red blood vessels Science Q Biology (General) QH301-705.5
spellingShingle	zebrafish adipocyte nile red blood vessels Science Q Biology (General) QH301-705.5 Paola Lepanto Florencia Levin-Ferreyra Uriel Koziol Leonel Malacrida José L. Badano Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
description	White adipose tissue hyperplasia has been shown to be crucial for handling excess energy in healthy ways. Though adipogenesis mechanisms have been underscored in vitro, we lack information on how tissue and systemic factors influence the differentiation of new adipocytes. While this could be studied in zebrafish, adipocyte identification currently relies on neutral lipid labeling, thus precluding access to cells in early stages of differentiation. Here we report the generation and analysis of a zebrafish line with the transgene fabp4a(-2.7):EGFPcaax. In vivo confocal microscopy of the pancreatic and abdominal visceral depots of transgenic larvae, revealed the presence of labeled mature adipocytes as well as immature cells in earlier stages of differentiation. Through co-labeling for blood vessels, we observed a close interaction of differentiating adipocytes with endothelial cells through cell protrusions. Finally, we implemented hyperspectral imaging and spectral phasor analysis in Nile Red-labeled transgenic larvae and revealed the lipid metabolic transition towards neutral lipid accumulation of differentiating adipocytes. Altogether our work presents the characterization of a novel adipocyte-specific label in zebrafish and uncovers previously unknown aspects of in vivo adipogenesis. This article has an associated First Person interview with the first author of the paper.
format	article
author	Paola Lepanto Florencia Levin-Ferreyra Uriel Koziol Leonel Malacrida José L. Badano
author_facet	Paola Lepanto Florencia Levin-Ferreyra Uriel Koziol Leonel Malacrida José L. Badano
author_sort	Paola Lepanto
title	Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
title_short	Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
title_full	Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
title_fullStr	Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
title_full_unstemmed	Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
title_sort	insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
publisher	The Company of Biologists
publishDate	2021
url	https://doaj.org/article/c8bbec3156d344d897d0cd5129adf7d2
work_keys_str_mv	AT paolalepanto insightsintoinvivoadipocytedifferentiationthroughcellspecificlabelinginzebrafish AT florencialevinferreyra insightsintoinvivoadipocytedifferentiationthroughcellspecificlabelinginzebrafish AT urielkoziol insightsintoinvivoadipocytedifferentiationthroughcellspecificlabelinginzebrafish AT leonelmalacrida insightsintoinvivoadipocytedifferentiationthroughcellspecificlabelinginzebrafish AT joselbadano insightsintoinvivoadipocytedifferentiationthroughcellspecificlabelinginzebrafish
_version_	1718407858376146944

Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish

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