A simple and cost-effective setup for super-resolution localization microscopy

Abstract Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standar...

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Main Authors:	Hongqiang Ma, Rao Fu, Jianquan Xu, Yang Liu
Format:	article
Language:	EN
Published:	Nature Portfolio 2017
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/c8ca032fc7f24d4c8c5cf61a09b93b09
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spelling	oai:doaj.org-article:c8ca032fc7f24d4c8c5cf61a09b93b092021-12-02T16:08:12ZA simple and cost-effective setup for super-resolution localization microscopy10.1038/s41598-017-01606-62045-2322https://doaj.org/article/c8ca032fc7f24d4c8c5cf61a09b93b092017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01606-6https://doaj.org/toc/2045-2322Abstract Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM setup can yield similar localization precision and spatial resolution compared to the standard SMLM equipped with state-of-the-art components, but at a small fraction of their cost. Our low-cost SMLM setup can potentially serve as a routine laboratory microscope with high-performance super-resolution imaging capability.Hongqiang MaRao FuJianquan XuYang LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongqiang Ma Rao Fu Jianquan Xu Yang Liu A simple and cost-effective setup for super-resolution localization microscopy
description	Abstract Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM setup can yield similar localization precision and spatial resolution compared to the standard SMLM equipped with state-of-the-art components, but at a small fraction of their cost. Our low-cost SMLM setup can potentially serve as a routine laboratory microscope with high-performance super-resolution imaging capability.
format	article
author	Hongqiang Ma Rao Fu Jianquan Xu Yang Liu
author_facet	Hongqiang Ma Rao Fu Jianquan Xu Yang Liu
author_sort	Hongqiang Ma
title	A simple and cost-effective setup for super-resolution localization microscopy
title_short	A simple and cost-effective setup for super-resolution localization microscopy
title_full	A simple and cost-effective setup for super-resolution localization microscopy
title_fullStr	A simple and cost-effective setup for super-resolution localization microscopy
title_full_unstemmed	A simple and cost-effective setup for super-resolution localization microscopy
title_sort	simple and cost-effective setup for super-resolution localization microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c8ca032fc7f24d4c8c5cf61a09b93b09
work_keys_str_mv	AT hongqiangma asimpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT raofu asimpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT jianquanxu asimpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT yangliu asimpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT hongqiangma simpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT raofu simpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT jianquanxu simpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy AT yangliu simpleandcosteffectivesetupforsuperresolutionlocalizationmicroscopy
_version_	1718384620488097792

A simple and cost-effective setup for super-resolution localization microscopy

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