Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells

Abstract While anaesthetics are frequently used on cancer patients during surgical procedures, their consequence on cancer progression remains to be elucidated. In this study, we sought to investigate the influence of local anesthetics on lung cancer cell dissemination in vitro and in vivo. A549 hum...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shun-Ming Chan, Bo-Feng Lin, Chih-Shung Wong, Wen-Ting Chuang, Yu-Ting Chou, Zhi-Fu Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c8dd0d86fb2f41c0b313b0b1787b96d5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c8dd0d86fb2f41c0b313b0b1787b96d5
record_format	dspace
spelling	oai:doaj.org-article:c8dd0d86fb2f41c0b313b0b1787b96d52021-12-02T15:05:57ZLevobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells10.1038/s41598-017-08885-z2045-2322https://doaj.org/article/c8dd0d86fb2f41c0b313b0b1787b96d52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08885-zhttps://doaj.org/toc/2045-2322Abstract While anaesthetics are frequently used on cancer patients during surgical procedures, their consequence on cancer progression remains to be elucidated. In this study, we sought to investigate the influence of local anesthetics on lung cancer cell dissemination in vitro and in vivo. A549 human non-small lung cancer cells were treated with various local anaesthetics including ropivacaine, lidocaine, levobupivacaine and bupivacaine. Cell barrier property was assessed using an electric cell-substrate impedance sensing (ECIS) system. The epithelial-to-mesenchymal transition (EMT) of treated cells was studied by immunofluorescence staining. In vitro and in vivo cancer cell dissemination were investigated.Gene expression microarray and quantitative real-time PCR (qrt-PCR) assays were used to identify the genes responsible for levobupivacaine-mediated cancer cell dissemination.The results illustrated that only levobupivacaine induced EMT in the treated cells and also caused the dissemination of cancer cells in vitro. In addition, after intravenous injection, levobupivacaine encouraged cancer cell dissemination in vivo. Gene expression microarray, qrt-PCR and immunoblotting revealed that after levobupivacaine treatment, the hypoxia-inducible factor (HIF)- 2α gene was upregulated in cancer cells. Our findings suggest that levobupivacaine may induce A549 lung cancer cell dissemination both in vitro and in vivo. More specifically, HIF-2α signaling possibly contributes to levobupivacaine-mediated A549 lung cancer cell dissemination.Shun-Ming ChanBo-Feng LinChih-Shung WongWen-Ting ChuangYu-Ting ChouZhi-Fu WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shun-Ming Chan Bo-Feng Lin Chih-Shung Wong Wen-Ting Chuang Yu-Ting Chou Zhi-Fu Wu Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
description	Abstract While anaesthetics are frequently used on cancer patients during surgical procedures, their consequence on cancer progression remains to be elucidated. In this study, we sought to investigate the influence of local anesthetics on lung cancer cell dissemination in vitro and in vivo. A549 human non-small lung cancer cells were treated with various local anaesthetics including ropivacaine, lidocaine, levobupivacaine and bupivacaine. Cell barrier property was assessed using an electric cell-substrate impedance sensing (ECIS) system. The epithelial-to-mesenchymal transition (EMT) of treated cells was studied by immunofluorescence staining. In vitro and in vivo cancer cell dissemination were investigated.Gene expression microarray and quantitative real-time PCR (qrt-PCR) assays were used to identify the genes responsible for levobupivacaine-mediated cancer cell dissemination.The results illustrated that only levobupivacaine induced EMT in the treated cells and also caused the dissemination of cancer cells in vitro. In addition, after intravenous injection, levobupivacaine encouraged cancer cell dissemination in vivo. Gene expression microarray, qrt-PCR and immunoblotting revealed that after levobupivacaine treatment, the hypoxia-inducible factor (HIF)- 2α gene was upregulated in cancer cells. Our findings suggest that levobupivacaine may induce A549 lung cancer cell dissemination both in vitro and in vivo. More specifically, HIF-2α signaling possibly contributes to levobupivacaine-mediated A549 lung cancer cell dissemination.
format	article
author	Shun-Ming Chan Bo-Feng Lin Chih-Shung Wong Wen-Ting Chuang Yu-Ting Chou Zhi-Fu Wu
author_facet	Shun-Ming Chan Bo-Feng Lin Chih-Shung Wong Wen-Ting Chuang Yu-Ting Chou Zhi-Fu Wu
author_sort	Shun-Ming Chan
title	Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_short	Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_full	Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_fullStr	Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_full_unstemmed	Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
title_sort	levobuipivacaine-induced dissemination of a549 lung cancer cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c8dd0d86fb2f41c0b313b0b1787b96d5
work_keys_str_mv	AT shunmingchan levobuipivacaineinduceddisseminationofa549lungcancercells AT bofenglin levobuipivacaineinduceddisseminationofa549lungcancercells AT chihshungwong levobuipivacaineinduceddisseminationofa549lungcancercells AT wentingchuang levobuipivacaineinduceddisseminationofa549lungcancercells AT yutingchou levobuipivacaineinduceddisseminationofa549lungcancercells AT zhifuwu levobuipivacaineinduceddisseminationofa549lungcancercells
_version_	1718388671065882624

Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells

Ejemplares similares