Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae

Abstract The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a...

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Autores principales:	K. T. Mulroney, J. M. Hall, X. Huang, E. Turnbull, N. M. Bzdyl, A. Chakera, U. Naseer, E. M. Corea, M. J. Ellington, K. L. Hopkins, A. L. Wester, O. Ekelund, N. Woodford, T. J. J. Inglis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c8de5f1abba94c5896a1a92b7189bafd
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spelling	oai:doaj.org-article:c8de5f1abba94c5896a1a92b7189bafd2021-12-02T12:32:15ZRapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae10.1038/s41598-017-02009-32045-2322https://doaj.org/article/c8de5f1abba94c5896a1a92b7189bafd2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02009-3https://doaj.org/toc/2045-2322Abstract The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew’s correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.K. T. MulroneyJ. M. HallX. HuangE. TurnbullN. M. BzdylA. ChakeraU. NaseerE. M. CoreaM. J. EllingtonK. L. HopkinsA. L. WesterO. EkelundN. WoodfordT. J. J. InglisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q K. T. Mulroney J. M. Hall X. Huang E. Turnbull N. M. Bzdyl A. Chakera U. Naseer E. M. Corea M. J. Ellington K. L. Hopkins A. L. Wester O. Ekelund N. Woodford T. J. J. Inglis Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae
description	Abstract The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew’s correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
format	article
author	K. T. Mulroney J. M. Hall X. Huang E. Turnbull N. M. Bzdyl A. Chakera U. Naseer E. M. Corea M. J. Ellington K. L. Hopkins A. L. Wester O. Ekelund N. Woodford T. J. J. Inglis
author_facet	K. T. Mulroney J. M. Hall X. Huang E. Turnbull N. M. Bzdyl A. Chakera U. Naseer E. M. Corea M. J. Ellington K. L. Hopkins A. L. Wester O. Ekelund N. Woodford T. J. J. Inglis
author_sort	K. T. Mulroney
title	Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae
title_short	Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae
title_full	Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae
title_fullStr	Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae
title_full_unstemmed	Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae
title_sort	rapid susceptibility profiling of carbapenem-resistant klebsiella pneumoniae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c8de5f1abba94c5896a1a92b7189bafd
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Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae

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