Low-dose phase retrieval of biological specimens using cryo-electron ptychography

Cryo-electron microscopy is widely employed in structural biology and uses phase contrast imaging. Here, the authors employ electron ptychography, a quantitative phase retrieval method for high-contrast, low-dose phase imaging of cryo-state rotavirus and immature HIV-1 virus-like particles, and show...

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Autores principales:	Liqi Zhou, Jingdong Song, Judy S. Kim, Xudong Pei, Chen Huang, Mark Boyce, Luiza Mendonça, Daniel Clare, Alistair Siebert, Christopher S. Allen, Emanuela Liberti, David Stuart, Xiaoqing Pan, Peter D. Nellist, Peijun Zhang, Angus I. Kirkland, Peng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c8ea3464125d437b83ebe9cf731aa745
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spelling	oai:doaj.org-article:c8ea3464125d437b83ebe9cf731aa7452021-12-02T15:57:18ZLow-dose phase retrieval of biological specimens using cryo-electron ptychography10.1038/s41467-020-16391-62041-1723https://doaj.org/article/c8ea3464125d437b83ebe9cf731aa7452020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16391-6https://doaj.org/toc/2041-1723Cryo-electron microscopy is widely employed in structural biology and uses phase contrast imaging. Here, the authors employ electron ptychography, a quantitative phase retrieval method for high-contrast, low-dose phase imaging of cryo-state rotavirus and immature HIV-1 virus-like particles, and show that electron ptychography is more efficient for phase recovery than conventional phase contrast imaging.Liqi ZhouJingdong SongJudy S. KimXudong PeiChen HuangMark BoyceLuiza MendonçaDaniel ClareAlistair SiebertChristopher S. AllenEmanuela LibertiDavid StuartXiaoqing PanPeter D. NellistPeijun ZhangAngus I. KirklandPeng WangNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Liqi Zhou Jingdong Song Judy S. Kim Xudong Pei Chen Huang Mark Boyce Luiza Mendonça Daniel Clare Alistair Siebert Christopher S. Allen Emanuela Liberti David Stuart Xiaoqing Pan Peter D. Nellist Peijun Zhang Angus I. Kirkland Peng Wang Low-dose phase retrieval of biological specimens using cryo-electron ptychography
description	Cryo-electron microscopy is widely employed in structural biology and uses phase contrast imaging. Here, the authors employ electron ptychography, a quantitative phase retrieval method for high-contrast, low-dose phase imaging of cryo-state rotavirus and immature HIV-1 virus-like particles, and show that electron ptychography is more efficient for phase recovery than conventional phase contrast imaging.
format	article
author	Liqi Zhou Jingdong Song Judy S. Kim Xudong Pei Chen Huang Mark Boyce Luiza Mendonça Daniel Clare Alistair Siebert Christopher S. Allen Emanuela Liberti David Stuart Xiaoqing Pan Peter D. Nellist Peijun Zhang Angus I. Kirkland Peng Wang
author_facet	Liqi Zhou Jingdong Song Judy S. Kim Xudong Pei Chen Huang Mark Boyce Luiza Mendonça Daniel Clare Alistair Siebert Christopher S. Allen Emanuela Liberti David Stuart Xiaoqing Pan Peter D. Nellist Peijun Zhang Angus I. Kirkland Peng Wang
author_sort	Liqi Zhou
title	Low-dose phase retrieval of biological specimens using cryo-electron ptychography
title_short	Low-dose phase retrieval of biological specimens using cryo-electron ptychography
title_full	Low-dose phase retrieval of biological specimens using cryo-electron ptychography
title_fullStr	Low-dose phase retrieval of biological specimens using cryo-electron ptychography
title_full_unstemmed	Low-dose phase retrieval of biological specimens using cryo-electron ptychography
title_sort	low-dose phase retrieval of biological specimens using cryo-electron ptychography
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c8ea3464125d437b83ebe9cf731aa745
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Low-dose phase retrieval of biological specimens using cryo-electron ptychography

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