Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition

The aim of the study was to define ultrastructural changes in the ovarian cyclic corpus luteum (CL) of dairy cows with different body condition score (BCS, a 5-point scale). The ovaries were recovered from slaughtered Holstein cows of BCS1 (emaciation; n= 4), BCS2 (tendency to emaciation; n=4), BCS3...

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Autores principales:	Pivko Juraj, Makarevich V. Alexander, Kubovičova Elena, Rafay Jan, Chrenek Peter
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	body condition score cattle emaciation fattening luteal regression Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/c8f35340a3f34501ba4ee8856cbae833
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spelling	oai:doaj.org-article:c8f35340a3f34501ba4ee8856cbae8332021-11-17T21:27:51ZUltrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition1820-744810.1515/acve-2016-0021https://doaj.org/article/c8f35340a3f34501ba4ee8856cbae8332016-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0021https://doaj.org/toc/1820-7448The aim of the study was to define ultrastructural changes in the ovarian cyclic corpus luteum (CL) of dairy cows with different body condition score (BCS, a 5-point scale). The ovaries were recovered from slaughtered Holstein cows of BCS1 (emaciation; n= 4), BCS2 (tendency to emaciation; n=4), BCS3 (average body condition; n= 4), BCS4 (tendency to fattening; n=4) and BCS5 (fattening; n=4). Average ages of cows were: 6.2, 4.1, 5.7, 5.5 and 6.36 years for BCS1, BCS2, BCS3, BCS4 and BCS5, respectively. For electron microscopy, functioning CL at the mid-luteal phase were fixed in the aldehyde solution and post-fixed in 1% OsO4 solution. For lipid visualization the samples were soaked in 1% p-phenylenediamine solution in acetone. Durcupan ultrathin sections (90 nm) were contrasted with uranyl acetate and lead citrate and viewed under Jeol transmission electron microscope. Occurrence of cell organelles was determined by counting the relative volume of individual cellular components using the modified universal test network. Luteal regression of cyclic CL was characterized by atrophy of lutein cells, condensation of organelles and cytoplasmic inclusions, increase in relative volumes of vacuoles, lysosomes, peroxisomes, autophagosomes and lipids. There were significant differences (p<0.05) among BCS1, BCS2, BCS4 and BCS5 cows in the occurrence of lipids, vacuoles, lysosomes and autophagosomes in the lutein cells compared to BCS3 cows. Cows with BCS2 or BCS1 had higher relative volume of lipid droplets, whilst the BCS4 or BCS5 cows had increased occurrence (relative volume) of vacuoles, lysosomes, peroxisomes and autolysosomes. These results show that luteolysis of cyclic CL of cows has different patterns in each BCS group; in the emaciated and fattened cows luteolysis starts earlier than in cows with average body condition.Pivko JurajMakarevich V. AlexanderKubovičova ElenaRafay JanChrenek PeterSciendoarticlebody condition scorecattleemaciationfatteningluteal regressionVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 2, Pp 245-256 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	body condition score cattle emaciation fattening luteal regression Veterinary medicine SF600-1100
spellingShingle	body condition score cattle emaciation fattening luteal regression Veterinary medicine SF600-1100 Pivko Juraj Makarevich V. Alexander Kubovičova Elena Rafay Jan Chrenek Peter Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
description	The aim of the study was to define ultrastructural changes in the ovarian cyclic corpus luteum (CL) of dairy cows with different body condition score (BCS, a 5-point scale). The ovaries were recovered from slaughtered Holstein cows of BCS1 (emaciation; n= 4), BCS2 (tendency to emaciation; n=4), BCS3 (average body condition; n= 4), BCS4 (tendency to fattening; n=4) and BCS5 (fattening; n=4). Average ages of cows were: 6.2, 4.1, 5.7, 5.5 and 6.36 years for BCS1, BCS2, BCS3, BCS4 and BCS5, respectively. For electron microscopy, functioning CL at the mid-luteal phase were fixed in the aldehyde solution and post-fixed in 1% OsO4 solution. For lipid visualization the samples were soaked in 1% p-phenylenediamine solution in acetone. Durcupan ultrathin sections (90 nm) were contrasted with uranyl acetate and lead citrate and viewed under Jeol transmission electron microscope. Occurrence of cell organelles was determined by counting the relative volume of individual cellular components using the modified universal test network. Luteal regression of cyclic CL was characterized by atrophy of lutein cells, condensation of organelles and cytoplasmic inclusions, increase in relative volumes of vacuoles, lysosomes, peroxisomes, autophagosomes and lipids. There were significant differences (p<0.05) among BCS1, BCS2, BCS4 and BCS5 cows in the occurrence of lipids, vacuoles, lysosomes and autophagosomes in the lutein cells compared to BCS3 cows. Cows with BCS2 or BCS1 had higher relative volume of lipid droplets, whilst the BCS4 or BCS5 cows had increased occurrence (relative volume) of vacuoles, lysosomes, peroxisomes and autolysosomes. These results show that luteolysis of cyclic CL of cows has different patterns in each BCS group; in the emaciated and fattened cows luteolysis starts earlier than in cows with average body condition.
format	article
author	Pivko Juraj Makarevich V. Alexander Kubovičova Elena Rafay Jan Chrenek Peter
author_facet	Pivko Juraj Makarevich V. Alexander Kubovičova Elena Rafay Jan Chrenek Peter
author_sort	Pivko Juraj
title	Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
title_short	Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
title_full	Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
title_fullStr	Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
title_full_unstemmed	Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition
title_sort	ultrastructural changes in the cyclic corpus luteum of dairy cows with different body condition
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/c8f35340a3f34501ba4ee8856cbae833
work_keys_str_mv	AT pivkojuraj ultrastructuralchangesinthecycliccorpusluteumofdairycowswithdifferentbodycondition AT makarevichvalexander ultrastructuralchangesinthecycliccorpusluteumofdairycowswithdifferentbodycondition AT kubovicovaelena ultrastructuralchangesinthecycliccorpusluteumofdairycowswithdifferentbodycondition AT rafayjan ultrastructuralchangesinthecycliccorpusluteumofdairycowswithdifferentbodycondition AT chrenekpeter ultrastructuralchangesinthecycliccorpusluteumofdairycowswithdifferentbodycondition
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Ultrastructural Changes in the Cyclic Corpus Luteum of Dairy Cows with Different Body Condition

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