Molecular heterogeneity in adjacent cells in triple-negative breast cancer

Michael L Huebschman,1 Nancy L Lane,1 Huaying Liu,1 Venetia R Sarode,2 Judith L Devlin,1 Eugene P Frenkel1,3 1Harold C Simmons Comprehensive Cancer Center, 2Department of Pathology, 3Division of Hematology-Medical Oncology, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX,...

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Autores principales:	Huebschman ML, Lane NL, Liu HY, Sarode VR, Devlin JL, Frenkel EP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/c8f3f56a3d4d4a618a5bb1edc5ff5b8a
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spelling	oai:doaj.org-article:c8f3f56a3d4d4a618a5bb1edc5ff5b8a2021-12-02T02:31:05ZMolecular heterogeneity in adjacent cells in triple-negative breast cancer1179-1314https://doaj.org/article/c8f3f56a3d4d4a618a5bb1edc5ff5b8a2015-08-01T00:00:00Zhttp://www.dovepress.com/molecular-heterogeneity-in-adjacent-cells-in-triple-negative-breast-ca-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Michael L Huebschman,1 Nancy L Lane,1 Huaying Liu,1 Venetia R Sarode,2 Judith L Devlin,1 Eugene P Frenkel1,3 1Harold C Simmons Comprehensive Cancer Center, 2Department of Pathology, 3Division of Hematology-Medical Oncology, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, USA Purpose: This study interrogates the molecular status of individual cells in patients with triple-negative breast cancers and explores the molecular identification and characterization of these tumors to consider the exploitation of a potential-targeted therapeutic approach. Patients and methods: Hyperspectral immunologic cell by cell analysis was applied to touch imprint smears obtained from fresh tumors of breast cancer patients. Results: Cell by cell analysis confirms significant intratumoral molecular heterogeneity in cancer markers with differences from polymerase chain reaction marker reporting. The individual cell heterogeneity was recognized in adjacent cells examined with panels of ten molecular markers in each single cell and included some markers that are considered to express “stem-cell” character. In addition, heterogeneity did not relate either to the size or stage of the primary tumor or to the site from within the cancer. Conclusion: There is a very significant molecular heterogeneity when “adjacent cells” are examined in triple-negative breast cancer, thereby making a successful targeted approach unlikely. In addition, it is not reasonable to consider that these changes will provide an answer to tumor dormancy. Keywords: hyperspectral, cancer stem cells, CSC, CD44, CD24, ALDH1, uPAR, CD133, Her-2Huebschman MLLane NLLiu HYSarode VRDevlin JLFrenkel EPDove Medical PressarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol 2015, Iss default, Pp 231-237 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Huebschman ML Lane NL Liu HY Sarode VR Devlin JL Frenkel EP Molecular heterogeneity in adjacent cells in triple-negative breast cancer
description	Michael L Huebschman,1 Nancy L Lane,1 Huaying Liu,1 Venetia R Sarode,2 Judith L Devlin,1 Eugene P Frenkel1,3 1Harold C Simmons Comprehensive Cancer Center, 2Department of Pathology, 3Division of Hematology-Medical Oncology, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, USA Purpose: This study interrogates the molecular status of individual cells in patients with triple-negative breast cancers and explores the molecular identification and characterization of these tumors to consider the exploitation of a potential-targeted therapeutic approach. Patients and methods: Hyperspectral immunologic cell by cell analysis was applied to touch imprint smears obtained from fresh tumors of breast cancer patients. Results: Cell by cell analysis confirms significant intratumoral molecular heterogeneity in cancer markers with differences from polymerase chain reaction marker reporting. The individual cell heterogeneity was recognized in adjacent cells examined with panels of ten molecular markers in each single cell and included some markers that are considered to express “stem-cell” character. In addition, heterogeneity did not relate either to the size or stage of the primary tumor or to the site from within the cancer. Conclusion: There is a very significant molecular heterogeneity when “adjacent cells” are examined in triple-negative breast cancer, thereby making a successful targeted approach unlikely. In addition, it is not reasonable to consider that these changes will provide an answer to tumor dormancy. Keywords: hyperspectral, cancer stem cells, CSC, CD44, CD24, ALDH1, uPAR, CD133, Her-2
format	article
author	Huebschman ML Lane NL Liu HY Sarode VR Devlin JL Frenkel EP
author_facet	Huebschman ML Lane NL Liu HY Sarode VR Devlin JL Frenkel EP
author_sort	Huebschman ML
title	Molecular heterogeneity in adjacent cells in triple-negative breast cancer
title_short	Molecular heterogeneity in adjacent cells in triple-negative breast cancer
title_full	Molecular heterogeneity in adjacent cells in triple-negative breast cancer
title_fullStr	Molecular heterogeneity in adjacent cells in triple-negative breast cancer
title_full_unstemmed	Molecular heterogeneity in adjacent cells in triple-negative breast cancer
title_sort	molecular heterogeneity in adjacent cells in triple-negative breast cancer
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/c8f3f56a3d4d4a618a5bb1edc5ff5b8a
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Molecular heterogeneity in adjacent cells in triple-negative breast cancer

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