Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation

Abstract Circular RNAs (circRNAs) are covalently closed circular non-coding RNAs. Due to their structure, circRNAs are more stable and have longer half-lives than linear RNAs making them good candidates for disease biomarkers. Despite the scientific relevance of these molecules, the study of circRNA...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Endika Varela-Martínez, Giulia I. Corsi, Christian Anthon, Jan Gorodkin, Begoña M. Jugo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c8f442d289464ac8acabedd39d7fec54
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c8f442d289464ac8acabedd39d7fec54
record_format	dspace
spelling	oai:doaj.org-article:c8f442d289464ac8acabedd39d7fec542021-12-02T14:01:33ZNovel circRNA discovery in sheep shows evidence of high backsplice junction conservation10.1038/s41598-020-79781-22045-2322https://doaj.org/article/c8f442d289464ac8acabedd39d7fec542021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79781-2https://doaj.org/toc/2045-2322Abstract Circular RNAs (circRNAs) are covalently closed circular non-coding RNAs. Due to their structure, circRNAs are more stable and have longer half-lives than linear RNAs making them good candidates for disease biomarkers. Despite the scientific relevance of these molecules, the study of circRNAs in non-model organisms is still in its infancy. Here, we analyse total RNA-seq data to identify circRNAs in sheep from peripheral blood mononuclear cells (PBMCs) and parietal lobe cortex. Out of 2510 and 3403 circRNAs detected in parietal lobe cortex and in PBMCs, a total of 1379 novel circRNAs were discovered. Remarkably, around 63% of all detected circRNAs were found to be completely homologous to a circRNA annotated in human. Functional enrichment analysis was conducted for both tissues based on GO terms and KEGG pathways. The enriched terms suggest an important role of circRNAs from encephalon in synaptic functions and the involvement of circRNAs from PBMCs in basic immune system functions. In addition to this, we investigated the role of circRNAs in repetitive vaccination experiments via differential expression analysis and did not detect any significant relationship. At last, our results support both the miRNA sponge and the miRNA shuttle functions of CDR1-AS in sheep brain. To our knowledge, this is the first study on circRNA annotation in sheep PBMCs or parietal lobe cortex samples.Endika Varela-MartínezGiulia I. CorsiChristian AnthonJan GorodkinBegoña M. JugoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Endika Varela-Martínez Giulia I. Corsi Christian Anthon Jan Gorodkin Begoña M. Jugo Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation
description	Abstract Circular RNAs (circRNAs) are covalently closed circular non-coding RNAs. Due to their structure, circRNAs are more stable and have longer half-lives than linear RNAs making them good candidates for disease biomarkers. Despite the scientific relevance of these molecules, the study of circRNAs in non-model organisms is still in its infancy. Here, we analyse total RNA-seq data to identify circRNAs in sheep from peripheral blood mononuclear cells (PBMCs) and parietal lobe cortex. Out of 2510 and 3403 circRNAs detected in parietal lobe cortex and in PBMCs, a total of 1379 novel circRNAs were discovered. Remarkably, around 63% of all detected circRNAs were found to be completely homologous to a circRNA annotated in human. Functional enrichment analysis was conducted for both tissues based on GO terms and KEGG pathways. The enriched terms suggest an important role of circRNAs from encephalon in synaptic functions and the involvement of circRNAs from PBMCs in basic immune system functions. In addition to this, we investigated the role of circRNAs in repetitive vaccination experiments via differential expression analysis and did not detect any significant relationship. At last, our results support both the miRNA sponge and the miRNA shuttle functions of CDR1-AS in sheep brain. To our knowledge, this is the first study on circRNA annotation in sheep PBMCs or parietal lobe cortex samples.
format	article
author	Endika Varela-Martínez Giulia I. Corsi Christian Anthon Jan Gorodkin Begoña M. Jugo
author_facet	Endika Varela-Martínez Giulia I. Corsi Christian Anthon Jan Gorodkin Begoña M. Jugo
author_sort	Endika Varela-Martínez
title	Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation
title_short	Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation
title_full	Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation
title_fullStr	Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation
title_full_unstemmed	Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation
title_sort	novel circrna discovery in sheep shows evidence of high backsplice junction conservation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c8f442d289464ac8acabedd39d7fec54
work_keys_str_mv	AT endikavarelamartinez novelcircrnadiscoveryinsheepshowsevidenceofhighbacksplicejunctionconservation AT giuliaicorsi novelcircrnadiscoveryinsheepshowsevidenceofhighbacksplicejunctionconservation AT christiananthon novelcircrnadiscoveryinsheepshowsevidenceofhighbacksplicejunctionconservation AT jangorodkin novelcircrnadiscoveryinsheepshowsevidenceofhighbacksplicejunctionconservation AT begonamjugo novelcircrnadiscoveryinsheepshowsevidenceofhighbacksplicejunctionconservation
_version_	1718392169265364992

Novel circRNA discovery in sheep shows evidence of high backsplice junction conservation

Ejemplares similares