Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations

Andrew Zane,1 Ranfang Zuo,2 Frederick A Villamena,3 Antal Rockenbauer,4,5 Ann Marie Digeorge Foushee,1 Kristin Flores,1 Prabir K Dutta,2 Amber Nagy1 1Biomaterials and Environmental Surveillance Department, Naval Medical Research Unit San Antonio, Joint Base San Antonio, Fort Sam Houston, San Antoni...

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Autores principales:	Zane A, Zuo RF, Villamena FA, Rockenbauer A, Digeorge Foushee AM, Flores K, Dutta PK, Nagy A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	titanium dioxide nitrogen doping antibacterial activity biocompatibility dental materials Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c8f98c8fecf942d8bb951b52d3abd5fa
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spelling	oai:doaj.org-article:c8f98c8fecf942d8bb951b52d3abd5fa2021-12-02T07:36:59ZBiocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations1178-2013https://doaj.org/article/c8f98c8fecf942d8bb951b52d3abd5fa2016-12-01T00:00:00Zhttps://www.dovepress.com/biocompatibility-and-antibacterial-activity-of-nitrogen-doped-titanium-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Andrew Zane,1 Ranfang Zuo,2 Frederick A Villamena,3 Antal Rockenbauer,4,5 Ann Marie Digeorge Foushee,1 Kristin Flores,1 Prabir K Dutta,2 Amber Nagy1 1Biomaterials and Environmental Surveillance Department, Naval Medical Research Unit San Antonio, Joint Base San Antonio, Fort Sam Houston, San Antonio, TX, 2Department of Chemistry and Biochemistry, 3Department of Biological Chemistry and Pharmacology, Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH, USA; 4Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Institute of Materials and Environmental Chemistry, 5Department of Physics, MTA-BME Condensed Matter Research Group, Budapest University of Technology and Economics, Budapest, Hungary Abstract: The addition of antibacterial functionality to dental resins presents an opportunity to extend their useful lifetime by reducing secondary caries caused by bacterial recolonization. In this study, the potential efficacy of nitrogen-doped titanium dioxide nanoparticles for this purpose was determined. Nitrogen doping was carried out to extend the ultraviolet absorbance into longer wavelength blue light for increased biocompatibility. Titanium dioxide nanoparticles (approximately 20–30 nm) were synthesized with and without nitrogen doping using a sol–gel method. Ultraviolet–Visible spectroscopy indicated a band of trap states, with increasing blue light absorbance as the concentration of the nitrogen dopant increased. Electron paramagnetic resonance measurements indicated the formation of superoxide and hydroxyl radicals upon particle exposure to visible light and oxygen. The particles were significantly toxic to Escherichia coli in a dose-dependent manner after a 1-hour exposure to a blue light source (480 nm). Intracellular reactive oxygen species assay demonstrated that the particles caused a stress response in human gingival epithelial cells when exposed to 1 hour of blue light, though this did not result in detectable release of cytokines. No decrease in cell viability was observed by water-soluble tetrazolium dye assay. The results show that nitrogen-doped titanium dioxide nanoparticles have antibacterial activity when exposed to blue light, and are biocompatible at these concentrations. Keywords: titanium dioxide, antibacterial activity, nitrogen dopingZane AZuo RFVillamena FARockenbauer ADigeorge Foushee AMFlores KDutta PKNagy ADove Medical Pressarticletitanium dioxidenitrogen dopingantibacterial activitybiocompatibilitydental materialsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6459-6470 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	titanium dioxide nitrogen doping antibacterial activity biocompatibility dental materials Medicine (General) R5-920
spellingShingle	titanium dioxide nitrogen doping antibacterial activity biocompatibility dental materials Medicine (General) R5-920 Zane A Zuo RF Villamena FA Rockenbauer A Digeorge Foushee AM Flores K Dutta PK Nagy A Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
description	Andrew Zane,1 Ranfang Zuo,2 Frederick A Villamena,3 Antal Rockenbauer,4,5 Ann Marie Digeorge Foushee,1 Kristin Flores,1 Prabir K Dutta,2 Amber Nagy1 1Biomaterials and Environmental Surveillance Department, Naval Medical Research Unit San Antonio, Joint Base San Antonio, Fort Sam Houston, San Antonio, TX, 2Department of Chemistry and Biochemistry, 3Department of Biological Chemistry and Pharmacology, Davis Heart and Lung Research Institute, College of Medicine, The Ohio State University, Columbus, OH, USA; 4Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Institute of Materials and Environmental Chemistry, 5Department of Physics, MTA-BME Condensed Matter Research Group, Budapest University of Technology and Economics, Budapest, Hungary Abstract: The addition of antibacterial functionality to dental resins presents an opportunity to extend their useful lifetime by reducing secondary caries caused by bacterial recolonization. In this study, the potential efficacy of nitrogen-doped titanium dioxide nanoparticles for this purpose was determined. Nitrogen doping was carried out to extend the ultraviolet absorbance into longer wavelength blue light for increased biocompatibility. Titanium dioxide nanoparticles (approximately 20–30 nm) were synthesized with and without nitrogen doping using a sol–gel method. Ultraviolet–Visible spectroscopy indicated a band of trap states, with increasing blue light absorbance as the concentration of the nitrogen dopant increased. Electron paramagnetic resonance measurements indicated the formation of superoxide and hydroxyl radicals upon particle exposure to visible light and oxygen. The particles were significantly toxic to Escherichia coli in a dose-dependent manner after a 1-hour exposure to a blue light source (480 nm). Intracellular reactive oxygen species assay demonstrated that the particles caused a stress response in human gingival epithelial cells when exposed to 1 hour of blue light, though this did not result in detectable release of cytokines. No decrease in cell viability was observed by water-soluble tetrazolium dye assay. The results show that nitrogen-doped titanium dioxide nanoparticles have antibacterial activity when exposed to blue light, and are biocompatible at these concentrations. Keywords: titanium dioxide, antibacterial activity, nitrogen doping
format	article
author	Zane A Zuo RF Villamena FA Rockenbauer A Digeorge Foushee AM Flores K Dutta PK Nagy A
author_facet	Zane A Zuo RF Villamena FA Rockenbauer A Digeorge Foushee AM Flores K Dutta PK Nagy A
author_sort	Zane A
title	Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
title_short	Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
title_full	Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
title_fullStr	Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
title_full_unstemmed	Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
title_sort	biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/c8f98c8fecf942d8bb951b52d3abd5fa
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Biocompatibility and antibacterial activity of nitrogen-doped titanium dioxide nanoparticles for use in dental resin formulations

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