Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Barbara Drašler,1 Damjana Drobne,1–3 Sara Novak,1 Janez Valant,1 Sabina Boljte,1,4 Lado Otrin,1 Michael Rappolt,5,6 Barbara Sartori,5 Aleš Iglic,7 Veronika Kralj-Iglic,8 Vid Šuštar,9 Darko Makovec,3,10 Sašo Gyergyek,10 Matej Hocevar,1...

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Autores principales: Drašler B, Drobne D, Novak S, Valant J, Boljte S, Otrin L, Rappolt M, Sartori B, Iglič A, Kralj-Iglič V, Šuštar V, Makovec D, Gyergyek S, Hočevar M, Godec M, Zupanc J
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/c90372dc98d5421f9821dc30f3ce32fe
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spelling oai:doaj.org-article:c90372dc98d5421f9821dc30f3ce32fe2021-12-02T07:42:38ZEffects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes1178-2013https://doaj.org/article/c90372dc98d5421f9821dc30f3ce32fe2014-03-01T00:00:00Zhttp://www.dovepress.com/effects-of-magnetic-cobalt-ferrite-nanoparticles-on-biological-and-art-a16253https://doaj.org/toc/1178-2013 Barbara Drašler,1 Damjana Drobne,1–3 Sara Novak,1 Janez Valant,1 Sabina Boljte,1,4 Lado Otrin,1 Michael Rappolt,5,6 Barbara Sartori,5 Aleš Iglic,7 Veronika Kralj-Iglic,8 Vid Šuštar,9 Darko Makovec,3,10 Sašo Gyergyek,10 Matej Hocevar,11 Matjaž Godec,11 Jernej Zupanc11University of Ljubljana, Biotechnical Faculty, Department of Biology, Ljubljana, 2Centre of Excellence in Advanced Materials and Technologies for the Future, Ljubljana, 3Centre of Excellence in Nanoscience and Nanotechnology, Ljubljana, 4Institute of Microbial Sciences and Technologies, Ljubljana, Slovenia; 5Institute of Inorganic Chemistry, Graz University of Technology, Basovizza, Italy; 6School of Food Science and Nutrition, University of Leeds, Leeds, UK; 7Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, 8Faculty of Health Sciences, Laboratory of Clinical Biophysics, University of Ljubljana, Ljubljana 9Laboratory of Clinical Biophysics, Chair of Orthopaedics, Faculty of Medicine, University of Ljubljana, Ljubljana, 10Institute Jožef Stefan, Ljubljana, 11Institute of Metals and Technology, Ljubljana, SloveniaBackground: The purpose of this work is to provide experimental evidence on the interactions of suspended nanoparticles with artificial or biological membranes and to assess the possibility of suspended nanoparticles interacting with the lipid component of biological membranes.Methods: 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid vesicles and human red blood cells were incubated in suspensions of magnetic bare cobalt ferrite (CoFe2O4) or citric acid (CA)-adsorbed CoFe2O4 nanoparticles dispersed in phosphate-buffered saline and glucose solution. The stability of POPC giant unilamellar vesicles after incubation in the tested nanoparticle suspensions was assessed by phase-contrast light microscopy and analyzed with computer-aided imaging. Structural changes in the POPC multilamellar vesicles were assessed by small angle X-ray scattering, and the shape transformation of red blood cells after incubation in tested suspensions of nanoparticles was observed using scanning electron microscopy and sedimentation, agglutination, and hemolysis assays.Results: Artificial lipid membranes were disturbed more by CA-adsorbed CoFe2O4 nanoparticle suspensions than by bare CoFe2O4 nanoparticle suspensions. CA-adsorbed CoFe2O4-CA nanoparticles caused more significant shape transformation in red blood cells than bare CoFe2O4 nanoparticles.Conclusion: Consistent with their smaller sized agglomerates, CA-adsorbed CoFe2O4 nanoparticles demonstrate more pronounced effects on artificial and biological membranes. Larger agglomerates of nanoparticles were confirmed to be reactive against lipid membranes and thus not acceptable for use with red blood cells. This finding is significant with respect to the efficient and safe application of nanoparticles as medicinal agents.Keywords: CoFe2O4, nanoparticles, agglomerates, human red blood cells, lipid vesiclesDrašler BDrobne DNovak SValant JBoljte SOtrin LRappolt MSartori BIglič AKralj-Iglič VŠuštar VMakovec DGyergyek SHočevar MGodec MZupanc JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1559-1581 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Drašler B
Drobne D
Novak S
Valant J
Boljte S
Otrin L
Rappolt M
Sartori B
Iglič A
Kralj-Iglič V
Šuštar V
Makovec D
Gyergyek S
Hočevar M
Godec M
Zupanc J
Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
description Barbara Drašler,1 Damjana Drobne,1–3 Sara Novak,1 Janez Valant,1 Sabina Boljte,1,4 Lado Otrin,1 Michael Rappolt,5,6 Barbara Sartori,5 Aleš Iglic,7 Veronika Kralj-Iglic,8 Vid Šuštar,9 Darko Makovec,3,10 Sašo Gyergyek,10 Matej Hocevar,11 Matjaž Godec,11 Jernej Zupanc11University of Ljubljana, Biotechnical Faculty, Department of Biology, Ljubljana, 2Centre of Excellence in Advanced Materials and Technologies for the Future, Ljubljana, 3Centre of Excellence in Nanoscience and Nanotechnology, Ljubljana, 4Institute of Microbial Sciences and Technologies, Ljubljana, Slovenia; 5Institute of Inorganic Chemistry, Graz University of Technology, Basovizza, Italy; 6School of Food Science and Nutrition, University of Leeds, Leeds, UK; 7Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, 8Faculty of Health Sciences, Laboratory of Clinical Biophysics, University of Ljubljana, Ljubljana 9Laboratory of Clinical Biophysics, Chair of Orthopaedics, Faculty of Medicine, University of Ljubljana, Ljubljana, 10Institute Jožef Stefan, Ljubljana, 11Institute of Metals and Technology, Ljubljana, SloveniaBackground: The purpose of this work is to provide experimental evidence on the interactions of suspended nanoparticles with artificial or biological membranes and to assess the possibility of suspended nanoparticles interacting with the lipid component of biological membranes.Methods: 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid vesicles and human red blood cells were incubated in suspensions of magnetic bare cobalt ferrite (CoFe2O4) or citric acid (CA)-adsorbed CoFe2O4 nanoparticles dispersed in phosphate-buffered saline and glucose solution. The stability of POPC giant unilamellar vesicles after incubation in the tested nanoparticle suspensions was assessed by phase-contrast light microscopy and analyzed with computer-aided imaging. Structural changes in the POPC multilamellar vesicles were assessed by small angle X-ray scattering, and the shape transformation of red blood cells after incubation in tested suspensions of nanoparticles was observed using scanning electron microscopy and sedimentation, agglutination, and hemolysis assays.Results: Artificial lipid membranes were disturbed more by CA-adsorbed CoFe2O4 nanoparticle suspensions than by bare CoFe2O4 nanoparticle suspensions. CA-adsorbed CoFe2O4-CA nanoparticles caused more significant shape transformation in red blood cells than bare CoFe2O4 nanoparticles.Conclusion: Consistent with their smaller sized agglomerates, CA-adsorbed CoFe2O4 nanoparticles demonstrate more pronounced effects on artificial and biological membranes. Larger agglomerates of nanoparticles were confirmed to be reactive against lipid membranes and thus not acceptable for use with red blood cells. This finding is significant with respect to the efficient and safe application of nanoparticles as medicinal agents.Keywords: CoFe2O4, nanoparticles, agglomerates, human red blood cells, lipid vesicles
format article
author Drašler B
Drobne D
Novak S
Valant J
Boljte S
Otrin L
Rappolt M
Sartori B
Iglič A
Kralj-Iglič V
Šuštar V
Makovec D
Gyergyek S
Hočevar M
Godec M
Zupanc J
author_facet Drašler B
Drobne D
Novak S
Valant J
Boljte S
Otrin L
Rappolt M
Sartori B
Iglič A
Kralj-Iglič V
Šuštar V
Makovec D
Gyergyek S
Hočevar M
Godec M
Zupanc J
author_sort Drašler B
title Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
title_short Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
title_full Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
title_fullStr Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
title_full_unstemmed Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
title_sort effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/c90372dc98d5421f9821dc30f3ce32fe
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