Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes

Abstract Keratinocytes, the main cell type of the skin, are one of the most exposed cells to environmental factors, providing a first defence barrier for the host and actively participating in immune response. In fact, keratinocytes express pattern recognition receptors that interact with pathogen a...

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Autores principales:	Sabrina Giantulli, Elisabetta Tortorella, Francesco Brasili, Susanna Scarpa, Barbara Cerroni, Gaio Paradossi, Angelico Bedini, Stefania Morrone, Ida Silvestri, Fabio Domenici
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9069442354f443f869e72c6120b0a2b
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spelling	oai:doaj.org-article:c9069442354f443f869e72c6120b0a2b2021-12-02T18:14:22ZEffect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes10.1038/s41598-021-98141-22045-2322https://doaj.org/article/c9069442354f443f869e72c6120b0a2b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98141-2https://doaj.org/toc/2045-2322Abstract Keratinocytes, the main cell type of the skin, are one of the most exposed cells to environmental factors, providing a first defence barrier for the host and actively participating in immune response. In fact, keratinocytes express pattern recognition receptors that interact with pathogen associated molecular patterns and damage associated molecular patterns, leading to the production of cytokines and chemokines, including interleukin (IL)-6. Herein, we investigated whether mechanical energy transported by low intensity ultrasound (US) could generate a mechanical stress able to induce the release of inflammatory cytokine such IL-6 in the human keratinocyte cell line, HaCaT. The extensive clinical application of US in both diagnosis and therapy suggests the need to better understand the related biological effects. Our results point out that US promotes the overexpression and secretion of IL-6, associated with the activation of nuclear factor-κB (NF-κB). Furthermore, we observed a reduced cell viability dependent on exposure parameters together with alterations in membrane permeability, paving the way for further investigating the molecular mechanisms related to US exposure.Sabrina GiantulliElisabetta TortorellaFrancesco BrasiliSusanna ScarpaBarbara CerroniGaio ParadossiAngelico BediniStefania MorroneIda SilvestriFabio DomeniciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabrina Giantulli Elisabetta Tortorella Francesco Brasili Susanna Scarpa Barbara Cerroni Gaio Paradossi Angelico Bedini Stefania Morrone Ida Silvestri Fabio Domenici Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
description	Abstract Keratinocytes, the main cell type of the skin, are one of the most exposed cells to environmental factors, providing a first defence barrier for the host and actively participating in immune response. In fact, keratinocytes express pattern recognition receptors that interact with pathogen associated molecular patterns and damage associated molecular patterns, leading to the production of cytokines and chemokines, including interleukin (IL)-6. Herein, we investigated whether mechanical energy transported by low intensity ultrasound (US) could generate a mechanical stress able to induce the release of inflammatory cytokine such IL-6 in the human keratinocyte cell line, HaCaT. The extensive clinical application of US in both diagnosis and therapy suggests the need to better understand the related biological effects. Our results point out that US promotes the overexpression and secretion of IL-6, associated with the activation of nuclear factor-κB (NF-κB). Furthermore, we observed a reduced cell viability dependent on exposure parameters together with alterations in membrane permeability, paving the way for further investigating the molecular mechanisms related to US exposure.
format	article
author	Sabrina Giantulli Elisabetta Tortorella Francesco Brasili Susanna Scarpa Barbara Cerroni Gaio Paradossi Angelico Bedini Stefania Morrone Ida Silvestri Fabio Domenici
author_facet	Sabrina Giantulli Elisabetta Tortorella Francesco Brasili Susanna Scarpa Barbara Cerroni Gaio Paradossi Angelico Bedini Stefania Morrone Ida Silvestri Fabio Domenici
author_sort	Sabrina Giantulli
title	Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
title_short	Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
title_full	Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
title_fullStr	Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
title_full_unstemmed	Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
title_sort	effect of 1-mhz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c9069442354f443f869e72c6120b0a2b
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Effect of 1-MHz ultrasound on the proinflammatory interleukin-6 secretion in human keratinocytes

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