Presence of distinctive microbiome in the first-pass meconium of newborn infants

Abstract We critically evaluated the fetal microbiome concept in 44 neonates with placenta, amniotic fluid, and first-pass meconium samples. Placental histology showed no signs of inflammation. Meconium samples were more often bacterial culture positive after vaginal delivery. In next-generation seq...

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Autores principales:	Jenni Turunen, Mysore V. Tejesvi, Niko Paalanne, Jenni Hekkala, Outi Lindgren, Mika Kaakinen, Tytti Pokka, Anna Kaisanlahti, Justus Reunanen, Terhi Tapiainen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c90a3ca5e6e74c9992cf01174bf824ae
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spelling	oai:doaj.org-article:c90a3ca5e6e74c9992cf01174bf824ae2021-12-02T17:37:35ZPresence of distinctive microbiome in the first-pass meconium of newborn infants10.1038/s41598-021-98951-42045-2322https://doaj.org/article/c90a3ca5e6e74c9992cf01174bf824ae2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98951-4https://doaj.org/toc/2045-2322Abstract We critically evaluated the fetal microbiome concept in 44 neonates with placenta, amniotic fluid, and first-pass meconium samples. Placental histology showed no signs of inflammation. Meconium samples were more often bacterial culture positive after vaginal delivery. In next-generation sequencing of the bacterial 16S gene, before and after removal of extracellular and PCR contaminant DNA, the median number of reads was low in placenta (48) and amniotic fluid (46) and high in meconium samples (14,556 C-section, 24,860 vaginal). In electron microscopy, meconium samples showed extracellular vesicles. Utilizing the analysis of composition of microbiomes (ANCOM) against water, meconium samples had a higher relative abundance of Firmicutes, Lactobacillus, Streptococcus, and Escherichia-Shigella. Our results did not support the existence of the placenta and amniotic fluid microbiota in healthy pregnancies. The first-pass meconium samples, formed in utero, appeared to harbor a microbiome that may be explained by perinatal colonization or intrauterine colonization via bacterial extracellular vesicles.Jenni TurunenMysore V. TejesviNiko PaalanneJenni HekkalaOuti LindgrenMika KaakinenTytti PokkaAnna KaisanlahtiJustus ReunanenTerhi TapiainenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jenni Turunen Mysore V. Tejesvi Niko Paalanne Jenni Hekkala Outi Lindgren Mika Kaakinen Tytti Pokka Anna Kaisanlahti Justus Reunanen Terhi Tapiainen Presence of distinctive microbiome in the first-pass meconium of newborn infants
description	Abstract We critically evaluated the fetal microbiome concept in 44 neonates with placenta, amniotic fluid, and first-pass meconium samples. Placental histology showed no signs of inflammation. Meconium samples were more often bacterial culture positive after vaginal delivery. In next-generation sequencing of the bacterial 16S gene, before and after removal of extracellular and PCR contaminant DNA, the median number of reads was low in placenta (48) and amniotic fluid (46) and high in meconium samples (14,556 C-section, 24,860 vaginal). In electron microscopy, meconium samples showed extracellular vesicles. Utilizing the analysis of composition of microbiomes (ANCOM) against water, meconium samples had a higher relative abundance of Firmicutes, Lactobacillus, Streptococcus, and Escherichia-Shigella. Our results did not support the existence of the placenta and amniotic fluid microbiota in healthy pregnancies. The first-pass meconium samples, formed in utero, appeared to harbor a microbiome that may be explained by perinatal colonization or intrauterine colonization via bacterial extracellular vesicles.
format	article
author	Jenni Turunen Mysore V. Tejesvi Niko Paalanne Jenni Hekkala Outi Lindgren Mika Kaakinen Tytti Pokka Anna Kaisanlahti Justus Reunanen Terhi Tapiainen
author_facet	Jenni Turunen Mysore V. Tejesvi Niko Paalanne Jenni Hekkala Outi Lindgren Mika Kaakinen Tytti Pokka Anna Kaisanlahti Justus Reunanen Terhi Tapiainen
author_sort	Jenni Turunen
title	Presence of distinctive microbiome in the first-pass meconium of newborn infants
title_short	Presence of distinctive microbiome in the first-pass meconium of newborn infants
title_full	Presence of distinctive microbiome in the first-pass meconium of newborn infants
title_fullStr	Presence of distinctive microbiome in the first-pass meconium of newborn infants
title_full_unstemmed	Presence of distinctive microbiome in the first-pass meconium of newborn infants
title_sort	presence of distinctive microbiome in the first-pass meconium of newborn infants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c90a3ca5e6e74c9992cf01174bf824ae
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Presence of distinctive microbiome in the first-pass meconium of newborn infants

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