Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).

The diversity and specificity of microbial communities in marine environments is a key aspect of the ecology and evolution of both the eukaryotic hosts and their associated prokaryotes. Marine sponges harbor phylogenetically diverse and complex microbial lineages. Here, we investigated the sponge ba...

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Autores principales:	Anoop Alex, Vitor Silva, Vitor Vasconcelos, Agostinho Antunes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c90eb69ef9bd441dbb758f6520fc1592
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spelling	oai:doaj.org-article:c90eb69ef9bd441dbb758f6520fc15922021-11-18T08:47:00ZEvidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).1932-620310.1371/journal.pone.0080653https://doaj.org/article/c90eb69ef9bd441dbb758f6520fc15922013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24265835/?tool=EBIhttps://doaj.org/toc/1932-6203The diversity and specificity of microbial communities in marine environments is a key aspect of the ecology and evolution of both the eukaryotic hosts and their associated prokaryotes. Marine sponges harbor phylogenetically diverse and complex microbial lineages. Here, we investigated the sponge bacterial community and distribution patterns of microbes in three sympatric intertidal marine demosponges, Hymeniacidon perlevis, Ophlitaspongia papilla and Polymastia penicillus, from the Atlantic coast of Portugal using classical isolation techniques and 16S rRNA gene clone libraries. Microbial composition assessment, with nearly full-length 16S rRNA gene sequences (ca. 1400 bp) from the isolates (n = 31) and partial sequences (ca. 280 bp) from clone libraries (n = 349), revealed diverse bacterial communities and other sponge-associated microbes. The majority of the bacterial isolates were members of the order Vibrionales and other symbiotic bacteria like Pseudovibrio ascidiaceiocola, Roseobacter sp., Hahellaceae sp. and Cobetia sp. Extended analyses using ecological metrics comprising 142 OTUs supported the clear differentiation of bacterial community profiles among the sponge hosts and their ambient seawater. Phylogenetic analyses were insightful in defining clades representing shared bacterial communities, particularly between H. perlevis and the geographically distantly-related H. heliophila, but also among other sponges. Furthermore, we also observed three distinct and unique bacterial groups, Betaproteobactria (~81%), Spirochaetes (~7%) and Chloroflexi (~3%), which are strictly maintained in low-microbial-abundance host species O. papilla and P. penicillus. Our study revealed the largely generalist nature of microbial associations among these co-occurring intertidal marine sponges.Anoop AlexVitor SilvaVitor VasconcelosAgostinho AntunesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80653 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anoop Alex Vitor Silva Vitor Vasconcelos Agostinho Antunes Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).
description	The diversity and specificity of microbial communities in marine environments is a key aspect of the ecology and evolution of both the eukaryotic hosts and their associated prokaryotes. Marine sponges harbor phylogenetically diverse and complex microbial lineages. Here, we investigated the sponge bacterial community and distribution patterns of microbes in three sympatric intertidal marine demosponges, Hymeniacidon perlevis, Ophlitaspongia papilla and Polymastia penicillus, from the Atlantic coast of Portugal using classical isolation techniques and 16S rRNA gene clone libraries. Microbial composition assessment, with nearly full-length 16S rRNA gene sequences (ca. 1400 bp) from the isolates (n = 31) and partial sequences (ca. 280 bp) from clone libraries (n = 349), revealed diverse bacterial communities and other sponge-associated microbes. The majority of the bacterial isolates were members of the order Vibrionales and other symbiotic bacteria like Pseudovibrio ascidiaceiocola, Roseobacter sp., Hahellaceae sp. and Cobetia sp. Extended analyses using ecological metrics comprising 142 OTUs supported the clear differentiation of bacterial community profiles among the sponge hosts and their ambient seawater. Phylogenetic analyses were insightful in defining clades representing shared bacterial communities, particularly between H. perlevis and the geographically distantly-related H. heliophila, but also among other sponges. Furthermore, we also observed three distinct and unique bacterial groups, Betaproteobactria (~81%), Spirochaetes (~7%) and Chloroflexi (~3%), which are strictly maintained in low-microbial-abundance host species O. papilla and P. penicillus. Our study revealed the largely generalist nature of microbial associations among these co-occurring intertidal marine sponges.
format	article
author	Anoop Alex Vitor Silva Vitor Vasconcelos Agostinho Antunes
author_facet	Anoop Alex Vitor Silva Vitor Vasconcelos Agostinho Antunes
author_sort	Anoop Alex
title	Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).
title_short	Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).
title_full	Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).
title_fullStr	Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).
title_full_unstemmed	Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).
title_sort	evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (porifera: demospongiae).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c90eb69ef9bd441dbb758f6520fc1592
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Evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (Porifera: Demospongiae).

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