Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)

Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock cultur...

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Autores principales:	Padeniya Uthpala, Septriani Shafira, Pataueg Arjay, Brown Christopher L.
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/c91232ee22d14a298885a106509bcb94
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spelling	oai:doaj.org-article:c91232ee22d14a298885a106509bcb942021-11-12T11:44:43ZEffects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)2267-124210.1051/e3sconf/202132202016https://doaj.org/article/c91232ee22d14a298885a106509bcb942021-01-01T00:00:00Zhttps://www.e3s-conferences.org/articles/e3sconf/pdf/2021/98/e3sconf_isffs2021_02016.pdfhttps://doaj.org/toc/2267-1242Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock culture tank as the control. Survival of the larvae throughout 10 dpf was recorded. To determine the development of the gastrointestinal tract DASPEI stain was used for larvae at the age of 3 dpf to 6dpf. The intensity of the fluorescence in each larva was observed through the automated digital microscope. According to the experiment results, a significant difference (P<0.0001) in the survival rates among all treatments was recorded. The probiotic-treated larvae (PTL) had a higher survival rate. This could be due to the presence of lactic acid bacteria in the probiotic treatment, which helps in enhancing immunity. In DASPEI staining, also PTL exhibits more fluorescence in the GI tract at 24 hours to 48 hours post-hatch than the other two treatments. The main possible reason behind this could also be the presence of Lactobacillus spp. Which directly influences the higher activity of the digestive system.Padeniya UthpalaSeptriani ShafiraPataueg ArjayBrown Christopher L.EDP SciencesarticleEnvironmental sciencesGE1-350ENFRE3S Web of Conferences, Vol 322, p 02016 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Environmental sciences GE1-350
spellingShingle	Environmental sciences GE1-350 Padeniya Uthpala Septriani Shafira Pataueg Arjay Brown Christopher L. Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
description	Microbial diversity within an aquatic community can be used to increase the growth and development of organisms. In this study, Zebrafish larvae were reared in three treatments 1) a probiotic containing 17 strains of Lactobacillus spp., 2) an amoxicillin solution, 3) water from the broodstock culture tank as the control. Survival of the larvae throughout 10 dpf was recorded. To determine the development of the gastrointestinal tract DASPEI stain was used for larvae at the age of 3 dpf to 6dpf. The intensity of the fluorescence in each larva was observed through the automated digital microscope. According to the experiment results, a significant difference (P<0.0001) in the survival rates among all treatments was recorded. The probiotic-treated larvae (PTL) had a higher survival rate. This could be due to the presence of lactic acid bacteria in the probiotic treatment, which helps in enhancing immunity. In DASPEI staining, also PTL exhibits more fluorescence in the GI tract at 24 hours to 48 hours post-hatch than the other two treatments. The main possible reason behind this could also be the presence of Lactobacillus spp. Which directly influences the higher activity of the digestive system.
format	article
author	Padeniya Uthpala Septriani Shafira Pataueg Arjay Brown Christopher L.
author_facet	Padeniya Uthpala Septriani Shafira Pataueg Arjay Brown Christopher L.
author_sort	Padeniya Uthpala
title	Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
title_short	Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
title_full	Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
title_fullStr	Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
title_full_unstemmed	Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)
title_sort	effects of the microbiome manipulation on survival and gi tract development of larval zebrafish (danio rerio)
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/c91232ee22d14a298885a106509bcb94
work_keys_str_mv	AT padeniyauthpala effectsofthemicrobiomemanipulationonsurvivalandgitractdevelopmentoflarvalzebrafishdaniorerio AT septrianishafira effectsofthemicrobiomemanipulationonsurvivalandgitractdevelopmentoflarvalzebrafishdaniorerio AT patauegarjay effectsofthemicrobiomemanipulationonsurvivalandgitractdevelopmentoflarvalzebrafishdaniorerio AT brownchristopherl effectsofthemicrobiomemanipulationonsurvivalandgitractdevelopmentoflarvalzebrafishdaniorerio
_version_	1718430579640238080

Effects of the microbiome manipulation on survival and GI tract development of larval zebrafish (Danio rerio)

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