Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study

A clear understanding of the properties of naturally induced antibody responses against transmission-blocking vaccine candidates can accelerate the understanding of the development of transmission-blocking immunity. This study characterized the naturally induced IgG responses against two leading tra...

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Autores principales:	Fermin K. Broni, Festus K. Acquah, Dorcas Obiri-Yeboah, Evans K. Obboh, Esther Sarpong, Linda E. Amoah
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	antibody levels relative avidity gametocyte P. falciparum polymerase chain reaction (PCR) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c918603453f946a8a3e79e67247446cd
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spelling	oai:doaj.org-article:c918603453f946a8a3e79e67247446cd2021-12-01T01:59:47ZProfiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study2235-298810.3389/fcimb.2021.770821https://doaj.org/article/c918603453f946a8a3e79e67247446cd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.770821/fullhttps://doaj.org/toc/2235-2988A clear understanding of the properties of naturally induced antibody responses against transmission-blocking vaccine candidates can accelerate the understanding of the development of transmission-blocking immunity. This study characterized the naturally induced IgG responses against two leading transmission-blocking vaccine antigens, Pfs230 and Pfs48/45, in non-febrile children living in Simiw, Ghana. Consecutive sampling was used to recruit 84 non-febrile children aged from 6 to 12 years old into the 6-month (November 2017 until May 2018) longitudinal study. Venous blood (1 ml) was collected once every 2 months and used to determine hemoglobin levels, P. falciparum prevalence using microscopy and polymerase chain reaction, and the levels and relative avidity of IgG responses against Pfs230 and Pfs48/45 using indirect ELISA. IgG levels against Pfs230 and Pfs48/45 decreased from the start (November) to the middle (January) and end (March) of the dry season respectively, then they began to increase. Participants, especially older children (10–12 years old) with active infections generally had lower antibody levels against both antigens. The relative avidities of IgG against both antigens followed the trend of IgG levels until the middle of the dry season, after which the relative avidities of both antigens correlated inversely with the antibody levels. In conclusion, although IgG antibody levels against both Pfs48/45 and Pfs230 began to increase by the early rainy season, they were inversely correlated to their respective relative avidities.Fermin K. BroniFestus K. AcquahFestus K. AcquahDorcas Obiri-YeboahDorcas Obiri-YeboahEvans K. ObbohEsther SarpongLinda E. AmoahLinda E. AmoahFrontiers Media S.A.articleantibody levelsrelative aviditygametocyteP. falciparumpolymerase chain reaction (PCR)MicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibody levels relative avidity gametocyte P. falciparum polymerase chain reaction (PCR) Microbiology QR1-502
spellingShingle	antibody levels relative avidity gametocyte P. falciparum polymerase chain reaction (PCR) Microbiology QR1-502 Fermin K. Broni Festus K. Acquah Festus K. Acquah Dorcas Obiri-Yeboah Dorcas Obiri-Yeboah Evans K. Obboh Esther Sarpong Linda E. Amoah Linda E. Amoah Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study
description	A clear understanding of the properties of naturally induced antibody responses against transmission-blocking vaccine candidates can accelerate the understanding of the development of transmission-blocking immunity. This study characterized the naturally induced IgG responses against two leading transmission-blocking vaccine antigens, Pfs230 and Pfs48/45, in non-febrile children living in Simiw, Ghana. Consecutive sampling was used to recruit 84 non-febrile children aged from 6 to 12 years old into the 6-month (November 2017 until May 2018) longitudinal study. Venous blood (1 ml) was collected once every 2 months and used to determine hemoglobin levels, P. falciparum prevalence using microscopy and polymerase chain reaction, and the levels and relative avidity of IgG responses against Pfs230 and Pfs48/45 using indirect ELISA. IgG levels against Pfs230 and Pfs48/45 decreased from the start (November) to the middle (January) and end (March) of the dry season respectively, then they began to increase. Participants, especially older children (10–12 years old) with active infections generally had lower antibody levels against both antigens. The relative avidities of IgG against both antigens followed the trend of IgG levels until the middle of the dry season, after which the relative avidities of both antigens correlated inversely with the antibody levels. In conclusion, although IgG antibody levels against both Pfs48/45 and Pfs230 began to increase by the early rainy season, they were inversely correlated to their respective relative avidities.
format	article
author	Fermin K. Broni Festus K. Acquah Festus K. Acquah Dorcas Obiri-Yeboah Dorcas Obiri-Yeboah Evans K. Obboh Esther Sarpong Linda E. Amoah Linda E. Amoah
author_facet	Fermin K. Broni Festus K. Acquah Festus K. Acquah Dorcas Obiri-Yeboah Dorcas Obiri-Yeboah Evans K. Obboh Esther Sarpong Linda E. Amoah Linda E. Amoah
author_sort	Fermin K. Broni
title	Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study
title_short	Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study
title_full	Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study
title_fullStr	Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study
title_full_unstemmed	Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study
title_sort	profiling the quality and quantity of naturally induced antibody responses against pfs230 and pfs48/45 among non-febrile children living in southern ghana: a longitudinal study
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c918603453f946a8a3e79e67247446cd
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Profiling the Quality and Quantity of Naturally Induced Antibody Responses Against Pfs230 and Pfs48/45 Among Non-Febrile Children Living in Southern Ghana: A Longitudinal Study

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