Chikungunya virus infections in Finnish travellers 2009-2019

The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened...

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Autores principales:	A. J. Jääskeläinen, L. Kareinen, T. Smura, H. Kallio-Kokko, O. Vapalahti
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	chikungunya rt-qpcr ngs thailand diagnostics Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/c918677768af41bf90cd7062f9bef67f
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spelling	oai:doaj.org-article:c918677768af41bf90cd7062f9bef67f2021-11-04T15:00:43ZChikungunya virus infections in Finnish travellers 2009-20192000-868610.1080/20008686.2020.1798096https://doaj.org/article/c918677768af41bf90cd7062f9bef67f2020-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20008686.2020.1798096https://doaj.org/toc/2000-8686The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened with the assay, followed by isolations attempts and sequencing using Sanger and next generation sequencing (NGS). To validate the assay external and in-house quality control samples were used and all were correctly identified. Specificity of the assay was 100%. Assay was sensitive to detect CHIKV RNA in dilution of 10-8.During years 2009-2019 34 patients were diagnosed for acute CHIKV infection. Twelve out of 34 cases were positive by CHIKV nsP2-RT-qPCR.Two CHIKV isolations succeeded from two individuals infected originally in Thailand, 2019. From 12 CHIKV nsP2-RT-qPCR positive samples, five (42%) CHIKVs were successfully sequenced. In this study, CHIKVs from year 2019 clustered with CHIKV ECSA-lineage forming sub-cluster with strains from ones detected in Bangladesh 2017, and the ones from Jamaica (2014) within Asian lineage showing highest similarity to strains detected in Caribbean outbreak 2013-15. Majority of the CHIKV infections detected in Finland originates from Asia and virus lineages reflect the global circulation of the pathogen.A. J. JääskeläinenL. KareinenT. SmuraH. Kallio-KokkoO. VapalahtiTaylor & Francis Grouparticlechikungunyart-qpcrngsthailanddiagnosticsInfectious and parasitic diseasesRC109-216ENInfection Ecology & Epidemiology, Vol 10, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	chikungunya rt-qpcr ngs thailand diagnostics Infectious and parasitic diseases RC109-216
spellingShingle	chikungunya rt-qpcr ngs thailand diagnostics Infectious and parasitic diseases RC109-216 A. J. Jääskeläinen L. Kareinen T. Smura H. Kallio-Kokko O. Vapalahti Chikungunya virus infections in Finnish travellers 2009-2019
description	The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened with the assay, followed by isolations attempts and sequencing using Sanger and next generation sequencing (NGS). To validate the assay external and in-house quality control samples were used and all were correctly identified. Specificity of the assay was 100%. Assay was sensitive to detect CHIKV RNA in dilution of 10-8.During years 2009-2019 34 patients were diagnosed for acute CHIKV infection. Twelve out of 34 cases were positive by CHIKV nsP2-RT-qPCR.Two CHIKV isolations succeeded from two individuals infected originally in Thailand, 2019. From 12 CHIKV nsP2-RT-qPCR positive samples, five (42%) CHIKVs were successfully sequenced. In this study, CHIKVs from year 2019 clustered with CHIKV ECSA-lineage forming sub-cluster with strains from ones detected in Bangladesh 2017, and the ones from Jamaica (2014) within Asian lineage showing highest similarity to strains detected in Caribbean outbreak 2013-15. Majority of the CHIKV infections detected in Finland originates from Asia and virus lineages reflect the global circulation of the pathogen.
format	article
author	A. J. Jääskeläinen L. Kareinen T. Smura H. Kallio-Kokko O. Vapalahti
author_facet	A. J. Jääskeläinen L. Kareinen T. Smura H. Kallio-Kokko O. Vapalahti
author_sort	A. J. Jääskeläinen
title	Chikungunya virus infections in Finnish travellers 2009-2019
title_short	Chikungunya virus infections in Finnish travellers 2009-2019
title_full	Chikungunya virus infections in Finnish travellers 2009-2019
title_fullStr	Chikungunya virus infections in Finnish travellers 2009-2019
title_full_unstemmed	Chikungunya virus infections in Finnish travellers 2009-2019
title_sort	chikungunya virus infections in finnish travellers 2009-2019
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/c918677768af41bf90cd7062f9bef67f
work_keys_str_mv	AT ajjaaskelainen chikungunyavirusinfectionsinfinnishtravellers20092019 AT lkareinen chikungunyavirusinfectionsinfinnishtravellers20092019 AT tsmura chikungunyavirusinfectionsinfinnishtravellers20092019 AT hkalliokokko chikungunyavirusinfectionsinfinnishtravellers20092019 AT ovapalahti chikungunyavirusinfectionsinfinnishtravellers20092019
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Chikungunya virus infections in Finnish travellers 2009-2019

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