Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.

Asymmetric positioning of the mitotic spindle in C. elegans embryos is mediated by force-generating complexes that are anchored at the plasma membrane and that pull on microtubules growing out from the spindle poles. Although asymmetric distribution of the force generators is thought to underlie asy...

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Autores principales:	Stefanie Redemann, Jacques Pecreaux, Nathan W Goehring, Khaled Khairy, Ernst H K Stelzer, Anthony A Hyman, Jonathon Howard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c91d7edfde4b4112ab409521dbf5b7f7
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spelling	oai:doaj.org-article:c91d7edfde4b4112ab409521dbf5b7f72021-11-18T06:35:50ZMembrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.1932-620310.1371/journal.pone.0012301https://doaj.org/article/c91d7edfde4b4112ab409521dbf5b7f72010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808841/?tool=EBIhttps://doaj.org/toc/1932-6203Asymmetric positioning of the mitotic spindle in C. elegans embryos is mediated by force-generating complexes that are anchored at the plasma membrane and that pull on microtubules growing out from the spindle poles. Although asymmetric distribution of the force generators is thought to underlie asymmetric positioning of the spindle, the number and location of the force generators has not been well defined. In particular, it has not been possible to visualize individual force generating events at the cortex. We discovered that perturbation of the acto-myosin cortex leads to the formation of long membrane invaginations that are pulled from the plasma membrane toward the spindle poles. Several lines of evidence show that the invaginations, which also occur in unperturbed embryos though at lower frequency, are pulled by the same force generators responsible for spindle positioning. Thus, the invaginations serve as a tool to localize the sites of force generation at the cortex and allow us to estimate a lower limit on the number of cortical force generators within the cell.Stefanie RedemannJacques PecreauxNathan W GoehringKhaled KhairyErnst H K StelzerAnthony A HymanJonathon HowardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12301 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefanie Redemann Jacques Pecreaux Nathan W Goehring Khaled Khairy Ernst H K Stelzer Anthony A Hyman Jonathon Howard Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.
description	Asymmetric positioning of the mitotic spindle in C. elegans embryos is mediated by force-generating complexes that are anchored at the plasma membrane and that pull on microtubules growing out from the spindle poles. Although asymmetric distribution of the force generators is thought to underlie asymmetric positioning of the spindle, the number and location of the force generators has not been well defined. In particular, it has not been possible to visualize individual force generating events at the cortex. We discovered that perturbation of the acto-myosin cortex leads to the formation of long membrane invaginations that are pulled from the plasma membrane toward the spindle poles. Several lines of evidence show that the invaginations, which also occur in unperturbed embryos though at lower frequency, are pulled by the same force generators responsible for spindle positioning. Thus, the invaginations serve as a tool to localize the sites of force generation at the cortex and allow us to estimate a lower limit on the number of cortical force generators within the cell.
format	article
author	Stefanie Redemann Jacques Pecreaux Nathan W Goehring Khaled Khairy Ernst H K Stelzer Anthony A Hyman Jonathon Howard
author_facet	Stefanie Redemann Jacques Pecreaux Nathan W Goehring Khaled Khairy Ernst H K Stelzer Anthony A Hyman Jonathon Howard
author_sort	Stefanie Redemann
title	Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.
title_short	Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.
title_full	Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.
title_fullStr	Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.
title_full_unstemmed	Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.
title_sort	membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell c. elegans embryos.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/c91d7edfde4b4112ab409521dbf5b7f7
work_keys_str_mv	AT stefanieredemann membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos AT jacquespecreaux membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos AT nathanwgoehring membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos AT khaledkhairy membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos AT ernsthkstelzer membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos AT anthonyahyman membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos AT jonathonhoward membraneinvaginationsrevealcorticalsitesthatpullonmitoticspindlesinonecellcelegansembryos
_version_	1718424445835542528

Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.

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