Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid

Abstract This study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64–68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control,...

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Autores principales:	Ramez H. Mahmoud, Claudia C. Biguetti, Gustavo B. Simionato, Isabela C. Custódio, Raquel B. P. Silva, Marco A. H. Duarte, Leonardo P. Faverani, Edilson Ervolino, Walid D. Fakhouri, Mariza A. Matsumoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c92b6c44f3f8470a9461da063e895e7e
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spelling	oai:doaj.org-article:c92b6c44f3f8470a9461da063e895e7e2021-12-02T19:16:54ZAlveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid10.1038/s41598-021-98713-22045-2322https://doaj.org/article/c92b6c44f3f8470a9461da063e895e7e2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98713-2https://doaj.org/toc/2045-2322Abstract This study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64–68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control, WT ZL, 5LOKO Control, and 5LOKO ZL. ZL groups were treated with an intraperitoneal injection of 250 µg/Kg of ZL, while controls were treated with saline. Treatments were administered once a week, starting four weeks before surgery for tooth extraction and until 7 and 21 days post-surgery. Mice were euthanized for a comprehensive microscopic analysis (microCT, histomorphometry and immunohistochemistry). WT ZL mice presented intense inflammatory infiltrate (7 days), delayed bone formation (21 days), reduced collagenous matrix quality, and a deficiency in Runx-2 + , TRAP + , and macrophages as compared to controls. 5LOKO ZL animals presented decreased number of Runx-2 + cells in comparison to 5LOKO Control at 7 days, but no major changes in bone healing as compared to WT or 5LOKO mice at 21 days. The knockout of 5LO favored intramembranous bone healing in aged female mice, with a direct impact on inflammatory response and bone metabolism on the development of ONJ-like lesions.Ramez H. MahmoudClaudia C. BiguettiGustavo B. SimionatoIsabela C. CustódioRaquel B. P. SilvaMarco A. H. DuarteLeonardo P. FaveraniEdilson ErvolinoWalid D. FakhouriMariza A. MatsumotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramez H. Mahmoud Claudia C. Biguetti Gustavo B. Simionato Isabela C. Custódio Raquel B. P. Silva Marco A. H. Duarte Leonardo P. Faverani Edilson Ervolino Walid D. Fakhouri Mariza A. Matsumoto Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
description	Abstract This study investigated the role 5-lypoxigenase (5-LO) on alveolar socket healing in aged female mice treated with zoledronic acid (ZL). Forty 129/Sv female mice (64–68 weeks old), 20 wild type (WT) and 20 5-LO knockout (5LOKO) were equally distributed according to ZL treatment: WT Control, WT ZL, 5LOKO Control, and 5LOKO ZL. ZL groups were treated with an intraperitoneal injection of 250 µg/Kg of ZL, while controls were treated with saline. Treatments were administered once a week, starting four weeks before surgery for tooth extraction and until 7 and 21 days post-surgery. Mice were euthanized for a comprehensive microscopic analysis (microCT, histomorphometry and immunohistochemistry). WT ZL mice presented intense inflammatory infiltrate (7 days), delayed bone formation (21 days), reduced collagenous matrix quality, and a deficiency in Runx-2 + , TRAP + , and macrophages as compared to controls. 5LOKO ZL animals presented decreased number of Runx-2 + cells in comparison to 5LOKO Control at 7 days, but no major changes in bone healing as compared to WT or 5LOKO mice at 21 days. The knockout of 5LO favored intramembranous bone healing in aged female mice, with a direct impact on inflammatory response and bone metabolism on the development of ONJ-like lesions.
format	article
author	Ramez H. Mahmoud Claudia C. Biguetti Gustavo B. Simionato Isabela C. Custódio Raquel B. P. Silva Marco A. H. Duarte Leonardo P. Faverani Edilson Ervolino Walid D. Fakhouri Mariza A. Matsumoto
author_facet	Ramez H. Mahmoud Claudia C. Biguetti Gustavo B. Simionato Isabela C. Custódio Raquel B. P. Silva Marco A. H. Duarte Leonardo P. Faverani Edilson Ervolino Walid D. Fakhouri Mariza A. Matsumoto
author_sort	Ramez H. Mahmoud
title	Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
title_short	Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
title_full	Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
title_fullStr	Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
title_full_unstemmed	Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
title_sort	alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c92b6c44f3f8470a9461da063e895e7e
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Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid

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