Computational model of membrane fission catalyzed by ESCRT-III.

ESCRT-III proteins catalyze membrane fission during multi vesicular body biogenesis, budding of some enveloped viruses and cell division. We suggest and analyze a novel mechanism of membrane fission by the mammalian ESCRT-III subunits CHMP2 and CHMP3. We propose that the CHMP2-CHMP3 complexes self-a...

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Autores principales: Gur Fabrikant, Suman Lata, James D Riches, John A G Briggs, Winfried Weissenhorn, Michael M Kozlov
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Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2009
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Acceso en línea:https://doaj.org/article/c9321f3bfc2b4dcf889f87856cc7d8fe
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spelling oai:doaj.org-article:c9321f3bfc2b4dcf889f87856cc7d8fe2021-11-25T05:42:50ZComputational model of membrane fission catalyzed by ESCRT-III.1553-734X1553-735810.1371/journal.pcbi.1000575https://doaj.org/article/c9321f3bfc2b4dcf889f87856cc7d8fe2009-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19936052/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358ESCRT-III proteins catalyze membrane fission during multi vesicular body biogenesis, budding of some enveloped viruses and cell division. We suggest and analyze a novel mechanism of membrane fission by the mammalian ESCRT-III subunits CHMP2 and CHMP3. We propose that the CHMP2-CHMP3 complexes self-assemble into hemi-spherical dome-like structures within the necks of the initial membrane buds generated by CHMP4 filaments. The dome formation is accompanied by the membrane attachment to the dome surface, which drives narrowing of the membrane neck and accumulation of the elastic stresses leading, ultimately, to the neck fission. Based on the bending elastic model of lipid bilayers, we determine the degree of the membrane attachment to the dome enabling the neck fission and compute the required values of the protein-membrane binding energy. We estimate the feasible values of this energy and predict a high efficiency for the CHMP2-CHMP3 complexes in mediating membrane fission. We support the computational model by electron tomography imaging of CHMP2-CHMP3 assemblies in vitro. We predict a high efficiency for the CHMP2-CHMP3 complexes in mediating membrane fission.Gur FabrikantSuman LataJames D RichesJohn A G BriggsWinfried WeissenhornMichael M KozlovPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 5, Iss 11, p e1000575 (2009)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Gur Fabrikant
Suman Lata
James D Riches
John A G Briggs
Winfried Weissenhorn
Michael M Kozlov
Computational model of membrane fission catalyzed by ESCRT-III.
description ESCRT-III proteins catalyze membrane fission during multi vesicular body biogenesis, budding of some enveloped viruses and cell division. We suggest and analyze a novel mechanism of membrane fission by the mammalian ESCRT-III subunits CHMP2 and CHMP3. We propose that the CHMP2-CHMP3 complexes self-assemble into hemi-spherical dome-like structures within the necks of the initial membrane buds generated by CHMP4 filaments. The dome formation is accompanied by the membrane attachment to the dome surface, which drives narrowing of the membrane neck and accumulation of the elastic stresses leading, ultimately, to the neck fission. Based on the bending elastic model of lipid bilayers, we determine the degree of the membrane attachment to the dome enabling the neck fission and compute the required values of the protein-membrane binding energy. We estimate the feasible values of this energy and predict a high efficiency for the CHMP2-CHMP3 complexes in mediating membrane fission. We support the computational model by electron tomography imaging of CHMP2-CHMP3 assemblies in vitro. We predict a high efficiency for the CHMP2-CHMP3 complexes in mediating membrane fission.
format article
author Gur Fabrikant
Suman Lata
James D Riches
John A G Briggs
Winfried Weissenhorn
Michael M Kozlov
author_facet Gur Fabrikant
Suman Lata
James D Riches
John A G Briggs
Winfried Weissenhorn
Michael M Kozlov
author_sort Gur Fabrikant
title Computational model of membrane fission catalyzed by ESCRT-III.
title_short Computational model of membrane fission catalyzed by ESCRT-III.
title_full Computational model of membrane fission catalyzed by ESCRT-III.
title_fullStr Computational model of membrane fission catalyzed by ESCRT-III.
title_full_unstemmed Computational model of membrane fission catalyzed by ESCRT-III.
title_sort computational model of membrane fission catalyzed by escrt-iii.
publisher Public Library of Science (PLoS)
publishDate 2009
url https://doaj.org/article/c9321f3bfc2b4dcf889f87856cc7d8fe
work_keys_str_mv AT gurfabrikant computationalmodelofmembranefissioncatalyzedbyescrtiii
AT sumanlata computationalmodelofmembranefissioncatalyzedbyescrtiii
AT jamesdriches computationalmodelofmembranefissioncatalyzedbyescrtiii
AT johnagbriggs computationalmodelofmembranefissioncatalyzedbyescrtiii
AT winfriedweissenhorn computationalmodelofmembranefissioncatalyzedbyescrtiii
AT michaelmkozlov computationalmodelofmembranefissioncatalyzedbyescrtiii
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