Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes

Strigiformes are affected by a substantial decline mainly caused by habitat loss and destruction, poaching, and trapping. Moreover, the increasing trend in bird trade and the growing interest in wild-caught rather than captive-bred birds are expected to encourage illegal trade. The biomolecular inve...

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Autores principales:	Patrizia Giangregorio, Lorenzo Naldi, Chiara Mengoni, Claudia Greco, Anna Padula, Marco Zaccaroni, Renato Fani, Giovanni Argenti, Nadia Mucci
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cross-amplification microsatellites Strigiformes forensic illegal trade kinship Genetics QH426-470
Acceso en línea:	https://doaj.org/article/c93b997fc10643419a9c4b62ae862a3c
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id	oai:doaj.org-article:c93b997fc10643419a9c4b62ae862a3c
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spelling	oai:doaj.org-article:c93b997fc10643419a9c4b62ae862a3c2021-11-25T17:41:15ZCross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes10.3390/genes121117212073-4425https://doaj.org/article/c93b997fc10643419a9c4b62ae862a3c2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1721https://doaj.org/toc/2073-4425Strigiformes are affected by a substantial decline mainly caused by habitat loss and destruction, poaching, and trapping. Moreover, the increasing trend in bird trade and the growing interest in wild-caught rather than captive-bred birds are expected to encourage illegal trade. The biomolecular investigation represents a valuable tool to track illegal trade and to explore the genetic variability to preserving biodiversity. Microsatellite loci (STRs) are the most used markers to study genetic variability. Despite the availability of species-specific microsatellite loci in Strigiformes, a unique panel permitting the description of the genetic variability across species has not been identified yet. We tested 32 highly polymorphic microsatellite markers to evaluate the reliability of a unique microsatellite panel in different species of Strigiformes and its use for conservation and forensic purposes. We included in the study 84 individuals belonging to 28 parental groups and 11 species of Strigiformes. After screening polymorphic microsatellite loci, the description of genetic variability, and the kinship assessment, we characterized a final panel of 12 microsatellite loci able to identify individuals in 9 Strigiformes species. This STR panel might support the authorities in the forensic investigation for suspected smugglers and false parental claims; moreover, it can be useful to evaluate relatedness among individuals in captive-bred populations and to implement research projects finalized to the description of the genetic variability in wild populations.Patrizia GiangregorioLorenzo NaldiChiara MengoniClaudia GrecoAnna PadulaMarco ZaccaroniRenato FaniGiovanni ArgentiNadia MucciMDPI AGarticlecross-amplificationmicrosatellitesStrigiformesforensicillegal tradekinshipGeneticsQH426-470ENGenes, Vol 12, Iss 1721, p 1721 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cross-amplification microsatellites Strigiformes forensic illegal trade kinship Genetics QH426-470
spellingShingle	cross-amplification microsatellites Strigiformes forensic illegal trade kinship Genetics QH426-470 Patrizia Giangregorio Lorenzo Naldi Chiara Mengoni Claudia Greco Anna Padula Marco Zaccaroni Renato Fani Giovanni Argenti Nadia Mucci Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes
description	Strigiformes are affected by a substantial decline mainly caused by habitat loss and destruction, poaching, and trapping. Moreover, the increasing trend in bird trade and the growing interest in wild-caught rather than captive-bred birds are expected to encourage illegal trade. The biomolecular investigation represents a valuable tool to track illegal trade and to explore the genetic variability to preserving biodiversity. Microsatellite loci (STRs) are the most used markers to study genetic variability. Despite the availability of species-specific microsatellite loci in Strigiformes, a unique panel permitting the description of the genetic variability across species has not been identified yet. We tested 32 highly polymorphic microsatellite markers to evaluate the reliability of a unique microsatellite panel in different species of Strigiformes and its use for conservation and forensic purposes. We included in the study 84 individuals belonging to 28 parental groups and 11 species of Strigiformes. After screening polymorphic microsatellite loci, the description of genetic variability, and the kinship assessment, we characterized a final panel of 12 microsatellite loci able to identify individuals in 9 Strigiformes species. This STR panel might support the authorities in the forensic investigation for suspected smugglers and false parental claims; moreover, it can be useful to evaluate relatedness among individuals in captive-bred populations and to implement research projects finalized to the description of the genetic variability in wild populations.
format	article
author	Patrizia Giangregorio Lorenzo Naldi Chiara Mengoni Claudia Greco Anna Padula Marco Zaccaroni Renato Fani Giovanni Argenti Nadia Mucci
author_facet	Patrizia Giangregorio Lorenzo Naldi Chiara Mengoni Claudia Greco Anna Padula Marco Zaccaroni Renato Fani Giovanni Argenti Nadia Mucci
author_sort	Patrizia Giangregorio
title	Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes
title_short	Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes
title_full	Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes
title_fullStr	Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes
title_full_unstemmed	Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes
title_sort	cross-amplification in strigiformes: a new str panel for forensic purposes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c93b997fc10643419a9c4b62ae862a3c
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Cross-Amplification in Strigiformes: A New STR Panel for Forensic Purposes

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