A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect

Objective: Osteoarthritis (OA) is a common degenerative joint disease, and animal models have proven pivotal in investigating this disease. This study aimed to develop a primate model of OA that may be more relevant to research studies on OA in humans.Method: Twelve female rhesus macaques were rando...

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Autores principales:	Xin Bi, Tao Li, Min Li, Shutian Xiang, Junhong Li, Bin Ling, Zhaoxiang Wu, Zhong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	knee osteoarthritis animal models rhesus macaques modified hulth method cartilage injury Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/c94f8fd8de3b4e5c94c3c8eef2441eb5
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spelling	oai:doaj.org-article:c94f8fd8de3b4e5c94c3c8eef2441eb52021-11-04T05:26:03ZA New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect2296-418510.3389/fbioe.2021.727643https://doaj.org/article/c94f8fd8de3b4e5c94c3c8eef2441eb52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.727643/fullhttps://doaj.org/toc/2296-4185Objective: Osteoarthritis (OA) is a common degenerative joint disease, and animal models have proven pivotal in investigating this disease. This study aimed to develop a primate model of OA that may be more relevant to research studies on OA in humans.Method: Twelve female rhesus macaques were randomly divided into three groups. Four animals were untreated (Control group); four were subjected to the modified Hulth method, involving cutting of the anterior and posterior cruciate ligaments, and transecting the meniscus (Hulth group); and four were subjected to the modified Hulth method combined with cartilage defect (MHCD group). Each primate was subjected to motor ability tests, and underwent arthroscopic, radiographic, morphological, and pathological observation of the knee joints at various times for up to 180 days.Results: Motor ability on Day 180 was significantly lower in the MHCD group than in the Control (p＜0.01) and Hulth (p＜0.05) groups. Radiographic and morphological examination showed that the severity of knee joint deformity and articular cartilage injury were greater in the MHCD group than in the other groups. Pathological examination showed that cartilage thickness was significantly lower in the MHCD group than in the other groups at the same time points. The Mankin score on Day 180 was markedly higher in the MHCD group than in the Hulth (p＜0.05) and Control (p＜0.001) groups.Conclusion: The MHCD model of OA closely resembles the pathophysiological processes of spontaneous knee OA in humans. The time required to develop knee OA is shorter using the MHCD model than using the Hulth method.Xin BiXin BiTao LiMin LiShutian XiangJunhong LiBin LingZhaoxiang WuZhong ChenFrontiers Media S.A.articlekneeosteoarthritisanimal modelsrhesus macaquesmodified hulth methodcartilage injuryBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	knee osteoarthritis animal models rhesus macaques modified hulth method cartilage injury Biotechnology TP248.13-248.65
spellingShingle	knee osteoarthritis animal models rhesus macaques modified hulth method cartilage injury Biotechnology TP248.13-248.65 Xin Bi Xin Bi Tao Li Min Li Shutian Xiang Junhong Li Bin Ling Zhaoxiang Wu Zhong Chen A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect
description	Objective: Osteoarthritis (OA) is a common degenerative joint disease, and animal models have proven pivotal in investigating this disease. This study aimed to develop a primate model of OA that may be more relevant to research studies on OA in humans.Method: Twelve female rhesus macaques were randomly divided into three groups. Four animals were untreated (Control group); four were subjected to the modified Hulth method, involving cutting of the anterior and posterior cruciate ligaments, and transecting the meniscus (Hulth group); and four were subjected to the modified Hulth method combined with cartilage defect (MHCD group). Each primate was subjected to motor ability tests, and underwent arthroscopic, radiographic, morphological, and pathological observation of the knee joints at various times for up to 180 days.Results: Motor ability on Day 180 was significantly lower in the MHCD group than in the Control (p＜0.01) and Hulth (p＜0.05) groups. Radiographic and morphological examination showed that the severity of knee joint deformity and articular cartilage injury were greater in the MHCD group than in the other groups. Pathological examination showed that cartilage thickness was significantly lower in the MHCD group than in the other groups at the same time points. The Mankin score on Day 180 was markedly higher in the MHCD group than in the Hulth (p＜0.05) and Control (p＜0.001) groups.Conclusion: The MHCD model of OA closely resembles the pathophysiological processes of spontaneous knee OA in humans. The time required to develop knee OA is shorter using the MHCD model than using the Hulth method.
format	article
author	Xin Bi Xin Bi Tao Li Min Li Shutian Xiang Junhong Li Bin Ling Zhaoxiang Wu Zhong Chen
author_facet	Xin Bi Xin Bi Tao Li Min Li Shutian Xiang Junhong Li Bin Ling Zhaoxiang Wu Zhong Chen
author_sort	Xin Bi
title	A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect
title_short	A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect
title_full	A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect
title_fullStr	A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect
title_full_unstemmed	A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect
title_sort	new method to develop the primate model of knee osteoarthritis with focal cartilage defect
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c94f8fd8de3b4e5c94c3c8eef2441eb5
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A New Method to Develop the Primate Model of Knee Osteoarthritis With Focal Cartilage Defect

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