Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation

<i>Lespedeza cuneata</i>, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural comp...

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Autores principales:	Heesun Kang, Min Jeong Yoo, Sang Ah Yi, Tae Wan Kim, Ji Won Ha, Myung Woo Na, Kun Hee Park, Seon-Hee Kim, Jeung-Whan Han, Tae Su Jang, Ki Hyun Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lespedeza cuneata</i> Fabaceae structural elucidation 3T3-L1 pre-adipocytes adipogenesis Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c95539f093654e3b9d643ac619cdfdfa
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spelling	oai:doaj.org-article:c95539f093654e3b9d643ac619cdfdfa2021-11-25T18:59:20ZPhytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation10.3390/separations81102032297-8739https://doaj.org/article/c95539f093654e3b9d643ac619cdfdfa2021-11-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/203https://doaj.org/toc/2297-8739<i>Lespedeza cuneata</i>, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural compounds with biological activities from Korean medicinal plants, the phytochemical investigation of <i>L. cuneata</i> resulted in the isolation of five chemical constituents: α-tocopherol (<b>1</b>), 7a-methoxy-α-tocopherol (<b>2</b>), 13(<i>R</i>)-hydroxy-octadeca-(9<i>Z</i>,11<i>E</i>,15<i>Z</i>)-trien-oic acid (<b>3</b>), α-dimorphecolic acid (<b>4</b>), and lupeol (<b>5</b>). The structural determination of the isolated compounds was elucidated from data gathered through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography–mass spectrometry (LC/MS). Until now, this study is the first to report these five compounds from the plant <i>L. cuneata</i>. Moreover, these isolated compounds (<b>1</b>–<b>5</b>) were evaluated for their anti-adipogenesis effects and their role in lipid metabolism during adipocyte maturation. As a result, the upregulation of mRNA expression levels of <i>Fabp4</i> from 3T3-L1 pre-adipocytes treated with compounds <b>3</b> and <b>4</b> demonstrated that these compounds efficiently induced adipocyte differentiation. Furthermore, compounds <b>3</b> and <b>4</b> were found to regulate lipid metabolism by the induction of lipolytic and of lipogenic gene expressions. Therefore, experimental data from these findings supported that the compounds <b>3</b> and <b>4</b> induce the adipogenesis of 3T3-L1 pre-adipocytes and regulate lipid metabolism.Heesun KangMin Jeong YooSang Ah YiTae Wan KimJi Won HaMyung Woo NaKun Hee ParkSeon-Hee KimJeung-Whan HanTae Su JangKi Hyun KimMDPI AGarticle<i>Lespedeza cuneata</i>Fabaceaestructural elucidation3T3-L1 pre-adipocytesadipogenesisPhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 203, p 203 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lespedeza cuneata</i> Fabaceae structural elucidation 3T3-L1 pre-adipocytes adipogenesis Physics QC1-999 Chemistry QD1-999
spellingShingle	<i>Lespedeza cuneata</i> Fabaceae structural elucidation 3T3-L1 pre-adipocytes adipogenesis Physics QC1-999 Chemistry QD1-999 Heesun Kang Min Jeong Yoo Sang Ah Yi Tae Wan Kim Ji Won Ha Myung Woo Na Kun Hee Park Seon-Hee Kim Jeung-Whan Han Tae Su Jang Ki Hyun Kim Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
description	<i>Lespedeza cuneata</i>, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural compounds with biological activities from Korean medicinal plants, the phytochemical investigation of <i>L. cuneata</i> resulted in the isolation of five chemical constituents: α-tocopherol (<b>1</b>), 7a-methoxy-α-tocopherol (<b>2</b>), 13(<i>R</i>)-hydroxy-octadeca-(9<i>Z</i>,11<i>E</i>,15<i>Z</i>)-trien-oic acid (<b>3</b>), α-dimorphecolic acid (<b>4</b>), and lupeol (<b>5</b>). The structural determination of the isolated compounds was elucidated from data gathered through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography–mass spectrometry (LC/MS). Until now, this study is the first to report these five compounds from the plant <i>L. cuneata</i>. Moreover, these isolated compounds (<b>1</b>–<b>5</b>) were evaluated for their anti-adipogenesis effects and their role in lipid metabolism during adipocyte maturation. As a result, the upregulation of mRNA expression levels of <i>Fabp4</i> from 3T3-L1 pre-adipocytes treated with compounds <b>3</b> and <b>4</b> demonstrated that these compounds efficiently induced adipocyte differentiation. Furthermore, compounds <b>3</b> and <b>4</b> were found to regulate lipid metabolism by the induction of lipolytic and of lipogenic gene expressions. Therefore, experimental data from these findings supported that the compounds <b>3</b> and <b>4</b> induce the adipogenesis of 3T3-L1 pre-adipocytes and regulate lipid metabolism.
format	article
author	Heesun Kang Min Jeong Yoo Sang Ah Yi Tae Wan Kim Ji Won Ha Myung Woo Na Kun Hee Park Seon-Hee Kim Jeung-Whan Han Tae Su Jang Ki Hyun Kim
author_facet	Heesun Kang Min Jeong Yoo Sang Ah Yi Tae Wan Kim Ji Won Ha Myung Woo Na Kun Hee Park Seon-Hee Kim Jeung-Whan Han Tae Su Jang Ki Hyun Kim
author_sort	Heesun Kang
title	Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
title_short	Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
title_full	Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
title_fullStr	Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
title_full_unstemmed	Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
title_sort	phytochemical constituents identified from the aerial parts of <i>lespedeza cuneata</i> and their effects on lipid metabolism during adipocyte maturation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c95539f093654e3b9d643ac619cdfdfa
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Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation

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