Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer

Abstract Melanoma is one of the most aggressive and deadly skin cancers, and although histopathological criteria are used for its prognosis, biomarkers are necessary to identify the different evolution stages. The applications of molecular imaging include the in vivo diagnosis of cancer with probes...

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Autores principales:	Estefanía Sicco, Amy Mónaco, Marcelo Fernandez, María Moreno, Victoria Calzada, Hugo Cerecetto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c958ece4803f481694033d1c6c09e6fd
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spelling	oai:doaj.org-article:c958ece4803f481694033d1c6c09e6fd2021-12-02T18:01:48ZMetastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer10.1038/s41598-021-98828-62045-2322https://doaj.org/article/c958ece4803f481694033d1c6c09e6fd2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98828-6https://doaj.org/toc/2045-2322Abstract Melanoma is one of the most aggressive and deadly skin cancers, and although histopathological criteria are used for its prognosis, biomarkers are necessary to identify the different evolution stages. The applications of molecular imaging include the in vivo diagnosis of cancer with probes that recognize the tumor-biomarkers specific expression allowing external image acquisitions and evaluation of the biological process in quali-quantitative ways. Aptamers are oligonucleotides that recognize targets with high affinity and specificity presenting advantages that make them interesting molecular imaging probes. Sgc8-c (DNA-aptamer) selectively recognizes PTK7-receptor overexpressed in various types of tumors. Herein, Sgc8-c was evaluated, for the first time, in a metastatic melanoma model as molecular imaging probe for in vivo diagnostic, as well as in a non-metastatic melanoma model. Firstly, two probes, radio- and fluorescent-probe, were in vitro evaluated verifying the high specific PTK7 recognition and its internalization in tumor cells by the endosomal route. Secondly, in vivo proof of concept was performed in animal tumor models. In addition, they have rapid clearance from blood exhibiting excellent target (tumor)/non-target organ ratios. Furthermore, optimal biodistribution was observed 24 h after probes injections accumulating almost exclusively in the tumor tissue. Sgc8-c is a potential tool for their specific use in the early detection of melanoma.Estefanía SiccoAmy MónacoMarcelo FernandezMaría MorenoVictoria CalzadaHugo CerecettoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Estefanía Sicco Amy Mónaco Marcelo Fernandez María Moreno Victoria Calzada Hugo Cerecetto Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer
description	Abstract Melanoma is one of the most aggressive and deadly skin cancers, and although histopathological criteria are used for its prognosis, biomarkers are necessary to identify the different evolution stages. The applications of molecular imaging include the in vivo diagnosis of cancer with probes that recognize the tumor-biomarkers specific expression allowing external image acquisitions and evaluation of the biological process in quali-quantitative ways. Aptamers are oligonucleotides that recognize targets with high affinity and specificity presenting advantages that make them interesting molecular imaging probes. Sgc8-c (DNA-aptamer) selectively recognizes PTK7-receptor overexpressed in various types of tumors. Herein, Sgc8-c was evaluated, for the first time, in a metastatic melanoma model as molecular imaging probe for in vivo diagnostic, as well as in a non-metastatic melanoma model. Firstly, two probes, radio- and fluorescent-probe, were in vitro evaluated verifying the high specific PTK7 recognition and its internalization in tumor cells by the endosomal route. Secondly, in vivo proof of concept was performed in animal tumor models. In addition, they have rapid clearance from blood exhibiting excellent target (tumor)/non-target organ ratios. Furthermore, optimal biodistribution was observed 24 h after probes injections accumulating almost exclusively in the tumor tissue. Sgc8-c is a potential tool for their specific use in the early detection of melanoma.
format	article
author	Estefanía Sicco Amy Mónaco Marcelo Fernandez María Moreno Victoria Calzada Hugo Cerecetto
author_facet	Estefanía Sicco Amy Mónaco Marcelo Fernandez María Moreno Victoria Calzada Hugo Cerecetto
author_sort	Estefanía Sicco
title	Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer
title_short	Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer
title_full	Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer
title_fullStr	Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer
title_full_unstemmed	Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer
title_sort	metastatic and non-metastatic melanoma imaging using sgc8-c aptamer ptk7-recognizer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c958ece4803f481694033d1c6c09e6fd
work_keys_str_mv	AT estefaniasicco metastaticandnonmetastaticmelanomaimagingusingsgc8captamerptk7recognizer AT amymonaco metastaticandnonmetastaticmelanomaimagingusingsgc8captamerptk7recognizer AT marcelofernandez metastaticandnonmetastaticmelanomaimagingusingsgc8captamerptk7recognizer AT mariamoreno metastaticandnonmetastaticmelanomaimagingusingsgc8captamerptk7recognizer AT victoriacalzada metastaticandnonmetastaticmelanomaimagingusingsgc8captamerptk7recognizer AT hugocerecetto metastaticandnonmetastaticmelanomaimagingusingsgc8captamerptk7recognizer
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Metastatic and non-metastatic melanoma imaging using Sgc8-c aptamer PTK7-recognizer

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