Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken.
Soluble fibres (non-starch polysaccharides, NSP) from edible plants but particularly plantain banana (Musa spp.), have been shown in vitro and ex vivo to prevent various enteric pathogens from adhering to, or translocating across, the human intestinal epithelium, a property that we have termed contr...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c9650eb50de44dbd8d9db0a1f8f63863 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c9650eb50de44dbd8d9db0a1f8f63863 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c9650eb50de44dbd8d9db0a1f8f638632021-11-18T08:34:14ZDietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken.1932-620310.1371/journal.pone.0087658https://doaj.org/article/c9650eb50de44dbd8d9db0a1f8f638632014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498347/?tool=EBIhttps://doaj.org/toc/1932-6203Soluble fibres (non-starch polysaccharides, NSP) from edible plants but particularly plantain banana (Musa spp.), have been shown in vitro and ex vivo to prevent various enteric pathogens from adhering to, or translocating across, the human intestinal epithelium, a property that we have termed contrabiotic. Here we report that dietary plantain fibre prevents invasion of the chicken intestinal mucosa by Salmonella. In vivo experiments were performed with chicks fed from hatch on a pellet diet containing soluble plantain NSP (0 to 200 mg/d) and orally infected with S.Typhimurium 4/74 at 8 d of age. Birds were sacrificed 3, 6 and 10 d post-infection. Bacteria were enumerated from liver, spleen and caecal contents. In vitro studies were performed using chicken caecal crypts and porcine intestinal epithelial cells infected with Salmonella enterica serovars following pre-treatment separately with soluble plantain NSP and acidic or neutral polysaccharide fractions of plantain NSP, each compared with saline vehicle. Bacterial adherence and invasion were assessed by gentamicin protection assay. In vivo dietary supplementation with plantain NSP 50 mg/d reduced invasion by S.Typhimurium, as reflected by viable bacterial counts from splenic tissue, by 98.9% (95% CI, 98.1-99.7; P<0.0001). In vitro studies confirmed that plantain NSP (5-10 mg/ml) inhibited adhesion of S.Typhimurium 4/74 to a porcine epithelial cell-line (73% mean inhibition (95% CI, 64-81); P<0.001) and to primary chick caecal crypts (82% mean inhibition (95% CI, 75-90); P<0.001). Adherence inhibition was shown to be mediated via an effect on the epithelial cells and Ussing chamber experiments with ex-vivo human ileal mucosa showed that this effect was associated with increased short circuit current but no change in electrical resistance. The inhibitory activity of plantain NSP lay mainly within the acidic/pectic (homogalacturonan-rich) component. Supplementation of chick feed with plantain NSP was well tolerated and shows promise as a simple approach for reducing invasive salmonellosis.Bryony N ParsonsPaul WigleyHannah L SimpsonJonathan M WilliamsSuzie HumphreyAnne-Marie SalisburyAlastair J M WatsonStephen C FryDavid O'BrienCarol L RobertsNiamh O'KennedyAsa V KeitaJohan D SöderholmJonathan M RhodesBarry J CampbellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87658 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Bryony N Parsons Paul Wigley Hannah L Simpson Jonathan M Williams Suzie Humphrey Anne-Marie Salisbury Alastair J M Watson Stephen C Fry David O'Brien Carol L Roberts Niamh O'Kennedy Asa V Keita Johan D Söderholm Jonathan M Rhodes Barry J Campbell Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken. |
| description |
Soluble fibres (non-starch polysaccharides, NSP) from edible plants but particularly plantain banana (Musa spp.), have been shown in vitro and ex vivo to prevent various enteric pathogens from adhering to, or translocating across, the human intestinal epithelium, a property that we have termed contrabiotic. Here we report that dietary plantain fibre prevents invasion of the chicken intestinal mucosa by Salmonella. In vivo experiments were performed with chicks fed from hatch on a pellet diet containing soluble plantain NSP (0 to 200 mg/d) and orally infected with S.Typhimurium 4/74 at 8 d of age. Birds were sacrificed 3, 6 and 10 d post-infection. Bacteria were enumerated from liver, spleen and caecal contents. In vitro studies were performed using chicken caecal crypts and porcine intestinal epithelial cells infected with Salmonella enterica serovars following pre-treatment separately with soluble plantain NSP and acidic or neutral polysaccharide fractions of plantain NSP, each compared with saline vehicle. Bacterial adherence and invasion were assessed by gentamicin protection assay. In vivo dietary supplementation with plantain NSP 50 mg/d reduced invasion by S.Typhimurium, as reflected by viable bacterial counts from splenic tissue, by 98.9% (95% CI, 98.1-99.7; P<0.0001). In vitro studies confirmed that plantain NSP (5-10 mg/ml) inhibited adhesion of S.Typhimurium 4/74 to a porcine epithelial cell-line (73% mean inhibition (95% CI, 64-81); P<0.001) and to primary chick caecal crypts (82% mean inhibition (95% CI, 75-90); P<0.001). Adherence inhibition was shown to be mediated via an effect on the epithelial cells and Ussing chamber experiments with ex-vivo human ileal mucosa showed that this effect was associated with increased short circuit current but no change in electrical resistance. The inhibitory activity of plantain NSP lay mainly within the acidic/pectic (homogalacturonan-rich) component. Supplementation of chick feed with plantain NSP was well tolerated and shows promise as a simple approach for reducing invasive salmonellosis. |
| format |
article |
| author |
Bryony N Parsons Paul Wigley Hannah L Simpson Jonathan M Williams Suzie Humphrey Anne-Marie Salisbury Alastair J M Watson Stephen C Fry David O'Brien Carol L Roberts Niamh O'Kennedy Asa V Keita Johan D Söderholm Jonathan M Rhodes Barry J Campbell |
| author_facet |
Bryony N Parsons Paul Wigley Hannah L Simpson Jonathan M Williams Suzie Humphrey Anne-Marie Salisbury Alastair J M Watson Stephen C Fry David O'Brien Carol L Roberts Niamh O'Kennedy Asa V Keita Johan D Söderholm Jonathan M Rhodes Barry J Campbell |
| author_sort |
Bryony N Parsons |
| title |
Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken. |
| title_short |
Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken. |
| title_full |
Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken. |
| title_fullStr |
Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken. |
| title_full_unstemmed |
Dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of Salmonella Typhimurium in the chicken. |
| title_sort |
dietary supplementation with soluble plantain non-starch polysaccharides inhibits intestinal invasion of salmonella typhimurium in the chicken. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/c9650eb50de44dbd8d9db0a1f8f63863 |
| work_keys_str_mv |
AT bryonynparsons dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT paulwigley dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT hannahlsimpson dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT jonathanmwilliams dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT suziehumphrey dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT annemariesalisbury dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT alastairjmwatson dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT stephencfry dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT davidobrien dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT carollroberts dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT niamhokennedy dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT asavkeita dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT johandsoderholm dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT jonathanmrhodes dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken AT barryjcampbell dietarysupplementationwithsolubleplantainnonstarchpolysaccharidesinhibitsintestinalinvasionofsalmonellatyphimuriuminthechicken |
| _version_ |
1718421647498674176 |