Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis

Phosphorylation of H2A at serine 95 (H2AS95ph) mediated by MLK4 promotes flowering and H2A.Z deposition. However, little is known about MLK1, MLK2, and MLK3 during the flowering time. Here, we systemically analyze the functions of MLK family in flowering time and development. Mutation in MLK3, but n...

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Autores principales:	Tongtong Huang, Heng Zhang, Yiming Zhou, Yanhua Su, Han Zheng, Yong Ding
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	histone phosphorylation flowering time GI MLK family phosphorylation of histone H2A at serine 95 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/c97451940bb64298b94f29d6fa447e11
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spelling	oai:doaj.org-article:c97451940bb64298b94f29d6fa447e112021-11-30T12:46:00ZPhosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis1664-462X10.3389/fpls.2021.761008https://doaj.org/article/c97451940bb64298b94f29d6fa447e112021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.761008/fullhttps://doaj.org/toc/1664-462XPhosphorylation of H2A at serine 95 (H2AS95ph) mediated by MLK4 promotes flowering and H2A.Z deposition. However, little is known about MLK1, MLK2, and MLK3 during the flowering time. Here, we systemically analyze the functions of MLK family in flowering time and development. Mutation in MLK3, but not MLK1 and MLK2, displayed late-flowering phenotype. Loss of MLK3 function enhanced the late-flowering phenotype of mlk4 mutant, but not reinforced the late-flowering phenotype of mlk1 mlk2 double mutants. MLK3 displayed the kinase activity to histone H2AS95ph in vitro. The global H2AS95ph levels were reduced in mlk3 mlk4, but not in mlk3 and mlk4 single mutant and mlk1 mlk2 double mutant, and the H2AS95ph levels in mlk1 mlk3 mlk4 and mlk2 mlk3 mlk4 were similar to those in mlk3 mlk4 double mutant. MLK3 interacted with CCA1, which binds to the promoter of GI. Correspondingly, the transcription levels and H2AS95ph levels of GI were reduced in mlk3 and mlk4 single mutant, and greatly decreased in mlk3 mlk4 double mutant, but not further attenuated in mlk1 mlk3 mlk4 and mlk2 mlk3 mlk4 triple mutant. Together, our results suggested that H2AS95ph deposition mediated by MLK3 and MLK4 is essential for flowering time in Arabidopsis.Tongtong HuangHeng ZhangYiming ZhouYanhua SuHan ZhengYong DingFrontiers Media S.A.articlehistone phosphorylationflowering timeGIMLK familyphosphorylation of histone H2A at serine 95Plant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	histone phosphorylation flowering time GI MLK family phosphorylation of histone H2A at serine 95 Plant culture SB1-1110
spellingShingle	histone phosphorylation flowering time GI MLK family phosphorylation of histone H2A at serine 95 Plant culture SB1-1110 Tongtong Huang Heng Zhang Yiming Zhou Yanhua Su Han Zheng Yong Ding Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis
description	Phosphorylation of H2A at serine 95 (H2AS95ph) mediated by MLK4 promotes flowering and H2A.Z deposition. However, little is known about MLK1, MLK2, and MLK3 during the flowering time. Here, we systemically analyze the functions of MLK family in flowering time and development. Mutation in MLK3, but not MLK1 and MLK2, displayed late-flowering phenotype. Loss of MLK3 function enhanced the late-flowering phenotype of mlk4 mutant, but not reinforced the late-flowering phenotype of mlk1 mlk2 double mutants. MLK3 displayed the kinase activity to histone H2AS95ph in vitro. The global H2AS95ph levels were reduced in mlk3 mlk4, but not in mlk3 and mlk4 single mutant and mlk1 mlk2 double mutant, and the H2AS95ph levels in mlk1 mlk3 mlk4 and mlk2 mlk3 mlk4 were similar to those in mlk3 mlk4 double mutant. MLK3 interacted with CCA1, which binds to the promoter of GI. Correspondingly, the transcription levels and H2AS95ph levels of GI were reduced in mlk3 and mlk4 single mutant, and greatly decreased in mlk3 mlk4 double mutant, but not further attenuated in mlk1 mlk3 mlk4 and mlk2 mlk3 mlk4 triple mutant. Together, our results suggested that H2AS95ph deposition mediated by MLK3 and MLK4 is essential for flowering time in Arabidopsis.
format	article
author	Tongtong Huang Heng Zhang Yiming Zhou Yanhua Su Han Zheng Yong Ding
author_facet	Tongtong Huang Heng Zhang Yiming Zhou Yanhua Su Han Zheng Yong Ding
author_sort	Tongtong Huang
title	Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis
title_short	Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis
title_full	Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis
title_fullStr	Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis
title_full_unstemmed	Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis
title_sort	phosphorylation of histone h2a at serine 95 is essential for flowering time and development in arabidopsis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c97451940bb64298b94f29d6fa447e11
work_keys_str_mv	AT tongtonghuang phosphorylationofhistoneh2aatserine95isessentialforfloweringtimeanddevelopmentinarabidopsis AT hengzhang phosphorylationofhistoneh2aatserine95isessentialforfloweringtimeanddevelopmentinarabidopsis AT yimingzhou phosphorylationofhistoneh2aatserine95isessentialforfloweringtimeanddevelopmentinarabidopsis AT yanhuasu phosphorylationofhistoneh2aatserine95isessentialforfloweringtimeanddevelopmentinarabidopsis AT hanzheng phosphorylationofhistoneh2aatserine95isessentialforfloweringtimeanddevelopmentinarabidopsis AT yongding phosphorylationofhistoneh2aatserine95isessentialforfloweringtimeanddevelopmentinarabidopsis
_version_	1718406589399957504

Phosphorylation of Histone H2A at Serine 95 Is Essential for Flowering Time and Development in Arabidopsis

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