Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections

Abstract Antibiotic resistance is spreading rapidly around the world and seriously impeding efforts to control microbial infections. Although nucleic acid testing is widely deployed for the detection of antibiotic resistant bacteria, the current techniques—mainly based on polymerase chain reaction (...

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Autores principales:	Yuye Wu, Dheerendranath Battalapalli, Mohammed J. Hakeem, Venkatarao Selamneni, Pengfei Zhang, Mohamed S. Draz, Zhi Ruan
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Bacteria CRISPR-Cas Antibiotic resistance Therapy Detection Delivery Biotechnology TP248.13-248.65 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/c97523bd5b1c46b88bf5f7f71e07ae36
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spelling	oai:doaj.org-article:c97523bd5b1c46b88bf5f7f71e07ae362021-12-05T12:19:07ZEngineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections10.1186/s12951-021-01132-81477-3155https://doaj.org/article/c97523bd5b1c46b88bf5f7f71e07ae362021-12-01T00:00:00Zhttps://doi.org/10.1186/s12951-021-01132-8https://doaj.org/toc/1477-3155Abstract Antibiotic resistance is spreading rapidly around the world and seriously impeding efforts to control microbial infections. Although nucleic acid testing is widely deployed for the detection of antibiotic resistant bacteria, the current techniques—mainly based on polymerase chain reaction (PCR)—are time-consuming and laborious. There is an urgent need to develop new strategies to control bacterial infections and the spread of antimicrobial resistance (AMR). The CRISPR-Cas system is an adaptive immune system found in many prokaryotes that presents attractive opportunities to target and edit nucleic acids with high precision and reliability. Engineered CRISPR-Cas systems are reported to effectively kill bacteria or even revert bacterial resistance to antibiotics (resensitizing bacterial cells to antibiotics). Strategies for combating antimicrobial resistance using CRISPR (i.e., Cas9, Cas12, Cas13, and Cas14) can be of great significance in detecting bacteria and their resistance to antibiotics. This review discusses the structures, mechanisms, and detection methods of CRISPR-Cas systems and how these systems can be engineered for the rapid and reliable detection of bacteria using various approaches, with a particular focus on nanoparticles. In addition, we summarize the most recent advances in applying the CRISPR-Cas system for virulence modulation of bacterial infections and combating antimicrobial resistance. Graphical AbstractYuye WuDheerendranath BattalapalliMohammed J. HakeemVenkatarao SelamneniPengfei ZhangMohamed S. DrazZhi RuanBMCarticleBacteriaCRISPR-CasAntibiotic resistanceTherapyDetectionDeliveryBiotechnologyTP248.13-248.65Medical technologyR855-855.5ENJournal of Nanobiotechnology, Vol 19, Iss 1, Pp 1-26 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacteria CRISPR-Cas Antibiotic resistance Therapy Detection Delivery Biotechnology TP248.13-248.65 Medical technology R855-855.5
spellingShingle	Bacteria CRISPR-Cas Antibiotic resistance Therapy Detection Delivery Biotechnology TP248.13-248.65 Medical technology R855-855.5 Yuye Wu Dheerendranath Battalapalli Mohammed J. Hakeem Venkatarao Selamneni Pengfei Zhang Mohamed S. Draz Zhi Ruan Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
description	Abstract Antibiotic resistance is spreading rapidly around the world and seriously impeding efforts to control microbial infections. Although nucleic acid testing is widely deployed for the detection of antibiotic resistant bacteria, the current techniques—mainly based on polymerase chain reaction (PCR)—are time-consuming and laborious. There is an urgent need to develop new strategies to control bacterial infections and the spread of antimicrobial resistance (AMR). The CRISPR-Cas system is an adaptive immune system found in many prokaryotes that presents attractive opportunities to target and edit nucleic acids with high precision and reliability. Engineered CRISPR-Cas systems are reported to effectively kill bacteria or even revert bacterial resistance to antibiotics (resensitizing bacterial cells to antibiotics). Strategies for combating antimicrobial resistance using CRISPR (i.e., Cas9, Cas12, Cas13, and Cas14) can be of great significance in detecting bacteria and their resistance to antibiotics. This review discusses the structures, mechanisms, and detection methods of CRISPR-Cas systems and how these systems can be engineered for the rapid and reliable detection of bacteria using various approaches, with a particular focus on nanoparticles. In addition, we summarize the most recent advances in applying the CRISPR-Cas system for virulence modulation of bacterial infections and combating antimicrobial resistance. Graphical Abstract
format	article
author	Yuye Wu Dheerendranath Battalapalli Mohammed J. Hakeem Venkatarao Selamneni Pengfei Zhang Mohamed S. Draz Zhi Ruan
author_facet	Yuye Wu Dheerendranath Battalapalli Mohammed J. Hakeem Venkatarao Selamneni Pengfei Zhang Mohamed S. Draz Zhi Ruan
author_sort	Yuye Wu
title	Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
title_short	Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
title_full	Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
title_fullStr	Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
title_full_unstemmed	Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
title_sort	engineered crispr-cas systems for the detection and control of antibiotic-resistant infections
publisher	BMC
publishDate	2021
url	https://doaj.org/article/c97523bd5b1c46b88bf5f7f71e07ae36
work_keys_str_mv	AT yuyewu engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections AT dheerendranathbattalapalli engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections AT mohammedjhakeem engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections AT venkataraoselamneni engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections AT pengfeizhang engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections AT mohamedsdraz engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections AT zhiruan engineeredcrisprcassystemsforthedetectionandcontrolofantibioticresistantinfections
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Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections

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