REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model

Eraldo Sanna Passino,1,2 Stefano Rocca,1 Sabrina Caggiu,1 Nicolò Columbano,1,2 Alessandro Castagna,3 Vania Fontani,3–5 Salvatore Rinaldi3–51Department of Veterinary Medicine, University of Sassari, Sassari, Italy; 2Comparative Surgery Research Laboratory, University of...

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Autores principales:	Sanna Passino E, Rocca S, Caggiu S, Columbano N, Castagna A, Fontani V, Rinaldi S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Aging Senescence Articular cartilage Regenerative medicine Regenerative physical treatments Radio Electric Asymmetric Conveyer. Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/c97866a3fd7f46a69baebd16ac4814e1
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spelling	oai:doaj.org-article:c97866a3fd7f46a69baebd16ac4814e12021-12-02T07:19:56ZREAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model1178-1998https://doaj.org/article/c97866a3fd7f46a69baebd16ac4814e12017-09-01T00:00:00Zhttps://www.dovepress.com/reac-regenerative-treatment-efficacy-in-experimental-chondral-lesions--peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Eraldo Sanna Passino,1,2 Stefano Rocca,1 Sabrina Caggiu,1 Nicolò Columbano,1,2 Alessandro Castagna,3 Vania Fontani,3–5 Salvatore Rinaldi3–51Department of Veterinary Medicine, University of Sassari, Sassari, Italy; 2Comparative Surgery Research Laboratory, University of Sassari, Sassari, Italy; 3Department of Regenerative Medicine, Rinaldi Fontani Institute, Florence, Italy; 4Research Department, Rinaldi Fontani Foundation, Florence, Italy; 5Research Department, IRF Shanghai Biomedical Sciences, Shanghai, People’s Republic of China Abstract: Radioelectric asymmetric conveyor (REAC) technology is a platform designed to optimize cell polarity. Cell polarity is a universal biological phenomenon that is implicated in cell differentiation, proliferation, morphogenesis, aging, and rejuvenation. In this work, we investigate a timing and administration protocol for tissue optimization regenerative treatment type C, in order to treat aging-related chondral damage or injuries and gain insights into regenerative processes of articular cartilage in humans. The chondral lesion produced in this study in an animal model (6 knee joints of 4 adult sheep) was 6 mm in diameter and about 2 mm deep. These lesions, which did not involve subchondral bone, tend to increase in size and depth and are not completely repaired with normal hyaline articular cartilage since adult articular cartilage is avascular and has a very slow turnover at the cellular and molecular level. Moreover, the hydration of articular cartilage is reduced with aging and with decreased mitotic activity, synthesis, and population size of chondrocytes. Six months posttreatment, lesions appeared filled, though not completely, with newly generated tissue of the light opalescent color of healthy articular cartilage, which otherwise covered the underlying subchondral bone. The newly formed tissue surface appeared to be quite regular. Nearly complete regeneration of subchondral bone occurred, with little vascularization and ossification nuclei almost absent. The results of this study confirm previous data obtained in vitro on the regenerative effects of REAC technology on human normal and osteoarthritic chondrocytes exposed to IL-1β. The present findings indicate that REAC tissue optimization-regenerative treatment type C is a promising therapeutic tool among the other REAC regenerative treatment protocols for the treatment of cartilage lesions. Keywords: aging, senescence, articular cartilage, regenerative medicine, regenerative physical treatments, radio electric asymmetric conveyerSanna Passino ERocca SCaggiu SColumbano NCastagna AFontani VRinaldi SDove Medical PressarticleAgingSenescenceArticular cartilageRegenerative medicineRegenerative physical treatmentsRadio Electric Asymmetric Conveyer.GeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 12, Pp 1471-1479 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aging Senescence Articular cartilage Regenerative medicine Regenerative physical treatments Radio Electric Asymmetric Conveyer. Geriatrics RC952-954.6
spellingShingle	Aging Senescence Articular cartilage Regenerative medicine Regenerative physical treatments Radio Electric Asymmetric Conveyer. Geriatrics RC952-954.6 Sanna Passino E Rocca S Caggiu S Columbano N Castagna A Fontani V Rinaldi S REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
description	Eraldo Sanna Passino,1,2 Stefano Rocca,1 Sabrina Caggiu,1 Nicolò Columbano,1,2 Alessandro Castagna,3 Vania Fontani,3–5 Salvatore Rinaldi3–51Department of Veterinary Medicine, University of Sassari, Sassari, Italy; 2Comparative Surgery Research Laboratory, University of Sassari, Sassari, Italy; 3Department of Regenerative Medicine, Rinaldi Fontani Institute, Florence, Italy; 4Research Department, Rinaldi Fontani Foundation, Florence, Italy; 5Research Department, IRF Shanghai Biomedical Sciences, Shanghai, People’s Republic of China Abstract: Radioelectric asymmetric conveyor (REAC) technology is a platform designed to optimize cell polarity. Cell polarity is a universal biological phenomenon that is implicated in cell differentiation, proliferation, morphogenesis, aging, and rejuvenation. In this work, we investigate a timing and administration protocol for tissue optimization regenerative treatment type C, in order to treat aging-related chondral damage or injuries and gain insights into regenerative processes of articular cartilage in humans. The chondral lesion produced in this study in an animal model (6 knee joints of 4 adult sheep) was 6 mm in diameter and about 2 mm deep. These lesions, which did not involve subchondral bone, tend to increase in size and depth and are not completely repaired with normal hyaline articular cartilage since adult articular cartilage is avascular and has a very slow turnover at the cellular and molecular level. Moreover, the hydration of articular cartilage is reduced with aging and with decreased mitotic activity, synthesis, and population size of chondrocytes. Six months posttreatment, lesions appeared filled, though not completely, with newly generated tissue of the light opalescent color of healthy articular cartilage, which otherwise covered the underlying subchondral bone. The newly formed tissue surface appeared to be quite regular. Nearly complete regeneration of subchondral bone occurred, with little vascularization and ossification nuclei almost absent. The results of this study confirm previous data obtained in vitro on the regenerative effects of REAC technology on human normal and osteoarthritic chondrocytes exposed to IL-1β. The present findings indicate that REAC tissue optimization-regenerative treatment type C is a promising therapeutic tool among the other REAC regenerative treatment protocols for the treatment of cartilage lesions. Keywords: aging, senescence, articular cartilage, regenerative medicine, regenerative physical treatments, radio electric asymmetric conveyer
format	article
author	Sanna Passino E Rocca S Caggiu S Columbano N Castagna A Fontani V Rinaldi S
author_facet	Sanna Passino E Rocca S Caggiu S Columbano N Castagna A Fontani V Rinaldi S
author_sort	Sanna Passino E
title	REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
title_short	REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
title_full	REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
title_fullStr	REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
title_full_unstemmed	REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
title_sort	reac regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/c97866a3fd7f46a69baebd16ac4814e1
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REAC regenerative treatment efficacy in experimental chondral lesions: a pilot study on ovine animal model

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