An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus

Abstract African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a fatal hemorrhagic disease of domestic pigs and wild boar. The virus primarily infects macrophage and monocyte host cells, these do not grow in vitro. Many attempts have been made to establish sustainab...

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Autores principales:	Kentaro Masujin, Tomoya Kitamura, Ken -ichiro Kameyama, Kota Okadera, Tatsuya Nishi, Takato Takenouchi, Hiroshi Kitani, Takehiro Kokuho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c97c92dadf5b4c90b260793cb1cf5030
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spelling	oai:doaj.org-article:c97c92dadf5b4c90b260793cb1cf50302021-12-02T15:52:59ZAn immortalized porcine macrophage cell line competent for the isolation of African swine fever virus10.1038/s41598-021-84237-22045-2322https://doaj.org/article/c97c92dadf5b4c90b260793cb1cf50302021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84237-2https://doaj.org/toc/2045-2322Abstract African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a fatal hemorrhagic disease of domestic pigs and wild boar. The virus primarily infects macrophage and monocyte host cells, these do not grow in vitro. Many attempts have been made to establish sustainable ASFV-sensitive cell lines, but which supported only low viral replication levels of limited, mostly artificially attenuated strains of ASFV. Here, we examined the competence of a novel cell line of immortalized porcine kidney macrophages (IPKM) for ASFV infection. We demonstrated that IPKM cells can facilitate high levels (> 107.0 TCID50/mL) of viral replication of ASFV, and hemadsorption reactions and cytopathic effects were observed as with porcine alveolar macrophages when inoculated with virulent field isolates: Armenia07, Kenya05/Tk-1, and Espana75. These results suggested that IPKM may be a valuable tool for the isolation, replication, and genetic manipulation of ASFV in both basic and applied ASF research.Kentaro MasujinTomoya KitamuraKen -ichiro KameyamaKota OkaderaTatsuya NishiTakato TakenouchiHiroshi KitaniTakehiro KokuhoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kentaro Masujin Tomoya Kitamura Ken -ichiro Kameyama Kota Okadera Tatsuya Nishi Takato Takenouchi Hiroshi Kitani Takehiro Kokuho An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
description	Abstract African swine fever virus (ASFV) is the etiological agent of African swine fever (ASF), a fatal hemorrhagic disease of domestic pigs and wild boar. The virus primarily infects macrophage and monocyte host cells, these do not grow in vitro. Many attempts have been made to establish sustainable ASFV-sensitive cell lines, but which supported only low viral replication levels of limited, mostly artificially attenuated strains of ASFV. Here, we examined the competence of a novel cell line of immortalized porcine kidney macrophages (IPKM) for ASFV infection. We demonstrated that IPKM cells can facilitate high levels (> 107.0 TCID50/mL) of viral replication of ASFV, and hemadsorption reactions and cytopathic effects were observed as with porcine alveolar macrophages when inoculated with virulent field isolates: Armenia07, Kenya05/Tk-1, and Espana75. These results suggested that IPKM may be a valuable tool for the isolation, replication, and genetic manipulation of ASFV in both basic and applied ASF research.
format	article
author	Kentaro Masujin Tomoya Kitamura Ken -ichiro Kameyama Kota Okadera Tatsuya Nishi Takato Takenouchi Hiroshi Kitani Takehiro Kokuho
author_facet	Kentaro Masujin Tomoya Kitamura Ken -ichiro Kameyama Kota Okadera Tatsuya Nishi Takato Takenouchi Hiroshi Kitani Takehiro Kokuho
author_sort	Kentaro Masujin
title	An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
title_short	An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
title_full	An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
title_fullStr	An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
title_full_unstemmed	An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus
title_sort	immortalized porcine macrophage cell line competent for the isolation of african swine fever virus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c97c92dadf5b4c90b260793cb1cf5030
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An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus

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