Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution

Abstract Acute normovolemic hemodilution (ANH) is a potential transfusion method for platelets, as well as for red blood cells. However, previous studies have shown that whole blood storage in ANH decreases platelet aggregability by 14.7–76.3% and that this decrease is not recovered by reinfusion. W...

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Autores principales:	Yutaka Murata, Eriko Kusudo, Shuji Kawamoto, Kazuhiko Fukuda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c98a67845b214680b00fdb3b8e9456e1
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spelling	oai:doaj.org-article:c98a67845b214680b00fdb3b8e9456e12021-12-02T15:03:07ZEffects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution10.1038/s41598-021-91725-y2045-2322https://doaj.org/article/c98a67845b214680b00fdb3b8e9456e12021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91725-yhttps://doaj.org/toc/2045-2322Abstract Acute normovolemic hemodilution (ANH) is a potential transfusion method for platelets, as well as for red blood cells. However, previous studies have shown that whole blood storage in ANH decreases platelet aggregability by 14.7–76.3% and that this decrease is not recovered by reinfusion. We investigated whether a new whole blood storage method for 6 h using a polyolefin bag, based on the platelet concentrates storage method, would maintain platelet function better than the conventional method using a polyvinyl chloride bag. We demonstrated that storage of whole blood in a polyolefin bag maintained ADP-induced aggregation rates at more than twofold higher than those in a polyvinyl chloride bag, and also significantly suppressed P-selectin expression, a platelet activation marker (ADP-induced aggregation rates: 24.6 ± 5.1% vs. 51.7 ± 11.5%, p = 0.002; P-selectin expression; 50.3 ± 8.4MFI vs. 31.6 ± 9.3MFI, p = 0.018). These results could be attributed to the high gas permeability of polyolefin, which lowered PCO2 and maintained a high pH with or without agitation. There were no significant changes in platelet count and red blood cell parameters due to the storage methods. Our results suggest that ANH using polyolefin bags is advantageous in improving hemostatic function compared to the conventional method.Yutaka MurataEriko KusudoShuji KawamotoKazuhiko FukudaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yutaka Murata Eriko Kusudo Shuji Kawamoto Kazuhiko Fukuda Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
description	Abstract Acute normovolemic hemodilution (ANH) is a potential transfusion method for platelets, as well as for red blood cells. However, previous studies have shown that whole blood storage in ANH decreases platelet aggregability by 14.7–76.3% and that this decrease is not recovered by reinfusion. We investigated whether a new whole blood storage method for 6 h using a polyolefin bag, based on the platelet concentrates storage method, would maintain platelet function better than the conventional method using a polyvinyl chloride bag. We demonstrated that storage of whole blood in a polyolefin bag maintained ADP-induced aggregation rates at more than twofold higher than those in a polyvinyl chloride bag, and also significantly suppressed P-selectin expression, a platelet activation marker (ADP-induced aggregation rates: 24.6 ± 5.1% vs. 51.7 ± 11.5%, p = 0.002; P-selectin expression; 50.3 ± 8.4MFI vs. 31.6 ± 9.3MFI, p = 0.018). These results could be attributed to the high gas permeability of polyolefin, which lowered PCO2 and maintained a high pH with or without agitation. There were no significant changes in platelet count and red blood cell parameters due to the storage methods. Our results suggest that ANH using polyolefin bags is advantageous in improving hemostatic function compared to the conventional method.
format	article
author	Yutaka Murata Eriko Kusudo Shuji Kawamoto Kazuhiko Fukuda
author_facet	Yutaka Murata Eriko Kusudo Shuji Kawamoto Kazuhiko Fukuda
author_sort	Yutaka Murata
title	Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
title_short	Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
title_full	Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
title_fullStr	Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
title_full_unstemmed	Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
title_sort	effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c98a67845b214680b00fdb3b8e9456e1
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Effects of whole blood storage in a polyolefin blood bag on platelets for acute normovolemic hemodilution

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