IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN

Objective: To evaluate the in vitro antifungal activity of Potash Alum (Phitkary) solution on Candida Albicans growth on heat cured acrylic resin. Study Design: Quasi-experimental study. Place and Duration of Study: Department of Oral Pathology, Peshawar Dental College, Peshawar, from Nov 2019...

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Autores principales:	Muhammad Irshad, Muhammad Younas, Hina Shabir, Muhammad Shiraz Alam, Zia Ur Rehman, Saima Aleem
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2020
Materias:	acrylic resin candida albicans denture cleaning agent potash alum Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c98f936ec4c34ec7bd71010c8ae1e849
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spelling	oai:doaj.org-article:c98f936ec4c34ec7bd71010c8ae1e8492021-12-02T17:20:32ZIN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN0030-96482411-8842https://doaj.org/article/c98f936ec4c34ec7bd71010c8ae1e8492020-10-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/5585https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To evaluate the in vitro antifungal activity of Potash Alum (Phitkary) solution on Candida Albicans growth on heat cured acrylic resin. Study Design: Quasi-experimental study. Place and Duration of Study: Department of Oral Pathology, Peshawar Dental College, Peshawar, from Nov 2019 to Jan 2020. Methodology: Acrylic resin discs (n=120) of standard size were prepared following the manufacturer’s instructions. The acrylic discs were contaminated with a suspension of 106cfus/ml of C. albicans in Sabouraud dextrose broth for 24 hours. After contamination, 30 acrylic discs were randomly selected, washed, sonicated, and plated for colony counts. The remaining 90 acrylic discs were randomly divided into 3 groups, 30 acrylic discs in each group. Group A discs were kept in 5.25% Sodium Hypochlrite solution, group B discs were kept in sterile Phosphate Buffered Saline and group C discs were kept in Potash-alum solution (10mg/ml). After 2 hours, all discs were washed, sonicated, and cultured on Sabouraud dextrose agar in serial dilutions for colony counts. Data was analysed using SPSS version-19. Results: Both Potash Alum (10 mg/ml) and Sodium Hypochlorite were effective in completely removing the attached Candida cells from the acrylic resin. In contrast, the negative control was only able to reduce viable counts by 23.6%. Sodium Hypochlorite and Potash Alum both showed statistically significant activity (p<0.01) against C. albicans compared to the negative control. Conclusion: Potash Alum has a significant in vitro antifungal activity against C. albicans on acrylic resin. Its antifungal effect is comparable to the effects of Sodium Hypochlorite.......Muhammad IrshadMuhammad YounasHina ShabirMuhammad Shiraz AlamZia Ur RehmanSaima AleemArmy Medical College Rawalpindiarticleacrylic resincandida albicansdenture cleaning agentpotash alumMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 70, Iss 5, Pp 1460-1464 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	acrylic resin candida albicans denture cleaning agent potash alum Medicine R Medicine (General) R5-920
spellingShingle	acrylic resin candida albicans denture cleaning agent potash alum Medicine R Medicine (General) R5-920 Muhammad Irshad Muhammad Younas Hina Shabir Muhammad Shiraz Alam Zia Ur Rehman Saima Aleem IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN
description	Objective: To evaluate the in vitro antifungal activity of Potash Alum (Phitkary) solution on Candida Albicans growth on heat cured acrylic resin. Study Design: Quasi-experimental study. Place and Duration of Study: Department of Oral Pathology, Peshawar Dental College, Peshawar, from Nov 2019 to Jan 2020. Methodology: Acrylic resin discs (n=120) of standard size were prepared following the manufacturer’s instructions. The acrylic discs were contaminated with a suspension of 106cfus/ml of C. albicans in Sabouraud dextrose broth for 24 hours. After contamination, 30 acrylic discs were randomly selected, washed, sonicated, and plated for colony counts. The remaining 90 acrylic discs were randomly divided into 3 groups, 30 acrylic discs in each group. Group A discs were kept in 5.25% Sodium Hypochlrite solution, group B discs were kept in sterile Phosphate Buffered Saline and group C discs were kept in Potash-alum solution (10mg/ml). After 2 hours, all discs were washed, sonicated, and cultured on Sabouraud dextrose agar in serial dilutions for colony counts. Data was analysed using SPSS version-19. Results: Both Potash Alum (10 mg/ml) and Sodium Hypochlorite were effective in completely removing the attached Candida cells from the acrylic resin. In contrast, the negative control was only able to reduce viable counts by 23.6%. Sodium Hypochlorite and Potash Alum both showed statistically significant activity (p<0.01) against C. albicans compared to the negative control. Conclusion: Potash Alum has a significant in vitro antifungal activity against C. albicans on acrylic resin. Its antifungal effect is comparable to the effects of Sodium Hypochlorite.......
format	article
author	Muhammad Irshad Muhammad Younas Hina Shabir Muhammad Shiraz Alam Zia Ur Rehman Saima Aleem
author_facet	Muhammad Irshad Muhammad Younas Hina Shabir Muhammad Shiraz Alam Zia Ur Rehman Saima Aleem
author_sort	Muhammad Irshad
title	IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN
title_short	IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN
title_full	IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN
title_fullStr	IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN
title_full_unstemmed	IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN
title_sort	in vitro antifungal activity of potash alum against candida albicans on acrylic resin
publisher	Army Medical College Rawalpindi
publishDate	2020
url	https://doaj.org/article/c98f936ec4c34ec7bd71010c8ae1e849
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IN VITRO ANTIFUNGAL ACTIVITY OF POTASH ALUM AGAINST CANDIDA ALBICANS ON ACRYLIC RESIN

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