Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines

Antibiotics are extensively used worldwide for the treatment of common infections by agents such as <i>E. coli</i> and <i>Salmonella.</i> They also represent the most common cause of alteration of the microbiota in people. We addressed whether broad-spectrum and Gram-negative...

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Autores principales:	Aklilu F. Haile, Rachel M. Woodfint, Eunsoo Kim, Marisa R. Joldrichsen, Nega Berhe, Wondwoossen A. Gebreyes, Prosper N. Boyaka
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibiotics IgA responses vaccines Medicine R
Acceso en línea:	https://doaj.org/article/c9996d9bcb5948c6a67e0217c70abf59
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spelling	oai:doaj.org-article:c9996d9bcb5948c6a67e0217c70abf592021-11-25T19:10:24ZBroad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines10.3390/vaccines91112402076-393Xhttps://doaj.org/article/c9996d9bcb5948c6a67e0217c70abf592021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1240https://doaj.org/toc/2076-393XAntibiotics are extensively used worldwide for the treatment of common infections by agents such as <i>E. coli</i> and <i>Salmonella.</i> They also represent the most common cause of alteration of the microbiota in people. We addressed whether broad-spectrum and Gram-negative-targeting antibiotics differentially regulate systemic and mucosal immune responses to vaccines. Antibiotics treatment enhances serum IgG1 responses in mice immunized systemically with a model polyvalent vaccine. This increase was not seen for other IgG subclasses and was dependent on the immunogenicity of vaccine antigens. The broad-spectrum antibiotic cocktail also enhanced serum IgA responses. Interestingly, both the broad spectrum and the antibiotic targeting Gram-negative bacteria enhanced the number of IgA antibody secreting cells in the intestinal lamina propria. This effect was unlikely to be due to an increase in cells expressing gut-homing receptors (i.e., CCR9 and α<sub>4</sub>β<sub>7</sub>) in peripheral tissues. On the other hand, the microbiome in mice treated with antibiotics was characterized by an overall reduction of the number of firmicutes. Furthermore, <i>Bacteroidetes</i> were increased by either treatment, and <i>Proteobacteria</i> were increased by the broad-spectrum antibiotics cocktail. Thus, immunoglobulin isotype and subclass responses are differentially regulated by oral antibiotics treatment and the gut microbiota shapes mucosal antibody responses after systemic immunization.Aklilu F. HaileRachel M. WoodfintEunsoo KimMarisa R. JoldrichsenNega BerheWondwoossen A. GebreyesProsper N. BoyakaMDPI AGarticleantibioticsIgA responsesvaccinesMedicineRENVaccines, Vol 9, Iss 1240, p 1240 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotics IgA responses vaccines Medicine R
spellingShingle	antibiotics IgA responses vaccines Medicine R Aklilu F. Haile Rachel M. Woodfint Eunsoo Kim Marisa R. Joldrichsen Nega Berhe Wondwoossen A. Gebreyes Prosper N. Boyaka Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
description	Antibiotics are extensively used worldwide for the treatment of common infections by agents such as <i>E. coli</i> and <i>Salmonella.</i> They also represent the most common cause of alteration of the microbiota in people. We addressed whether broad-spectrum and Gram-negative-targeting antibiotics differentially regulate systemic and mucosal immune responses to vaccines. Antibiotics treatment enhances serum IgG1 responses in mice immunized systemically with a model polyvalent vaccine. This increase was not seen for other IgG subclasses and was dependent on the immunogenicity of vaccine antigens. The broad-spectrum antibiotic cocktail also enhanced serum IgA responses. Interestingly, both the broad spectrum and the antibiotic targeting Gram-negative bacteria enhanced the number of IgA antibody secreting cells in the intestinal lamina propria. This effect was unlikely to be due to an increase in cells expressing gut-homing receptors (i.e., CCR9 and α<sub>4</sub>β<sub>7</sub>) in peripheral tissues. On the other hand, the microbiome in mice treated with antibiotics was characterized by an overall reduction of the number of firmicutes. Furthermore, <i>Bacteroidetes</i> were increased by either treatment, and <i>Proteobacteria</i> were increased by the broad-spectrum antibiotics cocktail. Thus, immunoglobulin isotype and subclass responses are differentially regulated by oral antibiotics treatment and the gut microbiota shapes mucosal antibody responses after systemic immunization.
format	article
author	Aklilu F. Haile Rachel M. Woodfint Eunsoo Kim Marisa R. Joldrichsen Nega Berhe Wondwoossen A. Gebreyes Prosper N. Boyaka
author_facet	Aklilu F. Haile Rachel M. Woodfint Eunsoo Kim Marisa R. Joldrichsen Nega Berhe Wondwoossen A. Gebreyes Prosper N. Boyaka
author_sort	Aklilu F. Haile
title	Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
title_short	Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
title_full	Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
title_fullStr	Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
title_full_unstemmed	Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
title_sort	broad-spectrum and gram-negative-targeting antibiotics differentially regulate antibody isotype responses to injected vaccines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c9996d9bcb5948c6a67e0217c70abf59
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Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines

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