Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing coronavirus disease (COVID-19) pandemic which is characterized by respiratory illness and severe pneumonia, and currently accounts for > 2.5 million deaths worldwide. Recently, diverse mutations in the spi...

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Autores principales:	Kaewta Rattanapisit, Christine Joy I Bulaon, Narach Khorattanakulchai, Balamurugan Shanmugaraj, Kittikhun Wangkanont, Waranyoo Phoolcharoen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c9a613561f4c42e29dd70651076412cb
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spelling	oai:doaj.org-article:c9a613561f4c42e29dd70651076412cb2021-12-02T20:18:26ZPlant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.1932-620310.1371/journal.pone.0253574https://doaj.org/article/c9a613561f4c42e29dd70651076412cb2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253574https://doaj.org/toc/1932-6203Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing coronavirus disease (COVID-19) pandemic which is characterized by respiratory illness and severe pneumonia, and currently accounts for > 2.5 million deaths worldwide. Recently, diverse mutations in the spike protein of SARS-CoV-2 were reported in United Kingdom (Alpha) and South Africa (Beta) strains which raise concerns over the potential increase in binding affinity towards the host cell receptor and diminished host neutralization capabilities. In order to study the effect of mutation in the binding efficiency of SARS-CoV-2 receptor binding domain (RBD) with anti-SARS-CoV/CoV-2 monoclonal antibodies (mAbs), we have produced SARS-CoV-2 RBD and two variants SARS-CoV-2 RBD (Alpha RBD and Beta RBD) in Nicotiana benthamiana by transient expression. Plant-produced SARS-CoV-2 RBD-Fc, Alpha RBD-Fc and Beta RBD-Fc exhibited specific binding to human angiotensin converting enzyme 2 (ACE2) receptor determined by ELISA. Intriguingly, the binding of plant-produced SARS-CoV-2 RBD proteins to plant-produced mAbs CR3022, B38, and H4 was found to be different depending on the variant mutation. In contrary to the plant-produced SARS-CoV-2 RBD-Fc and Alpha RBD-Fc, Beta RBD-Fc variant showed weak binding affinity towards the mAbs. The result suggested that the Beta RBD variant might have acquired partial resistance to neutralizing antibodies compared to other variants. However, further studies with sera from convalescent or vaccinated individuals are required to confirm this finding.Kaewta RattanapisitChristine Joy I BulaonNarach KhorattanakulchaiBalamurugan ShanmugarajKittikhun WangkanontWaranyoo PhoolcharoenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0253574 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kaewta Rattanapisit Christine Joy I Bulaon Narach Khorattanakulchai Balamurugan Shanmugaraj Kittikhun Wangkanont Waranyoo Phoolcharoen Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
description	Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing coronavirus disease (COVID-19) pandemic which is characterized by respiratory illness and severe pneumonia, and currently accounts for > 2.5 million deaths worldwide. Recently, diverse mutations in the spike protein of SARS-CoV-2 were reported in United Kingdom (Alpha) and South Africa (Beta) strains which raise concerns over the potential increase in binding affinity towards the host cell receptor and diminished host neutralization capabilities. In order to study the effect of mutation in the binding efficiency of SARS-CoV-2 receptor binding domain (RBD) with anti-SARS-CoV/CoV-2 monoclonal antibodies (mAbs), we have produced SARS-CoV-2 RBD and two variants SARS-CoV-2 RBD (Alpha RBD and Beta RBD) in Nicotiana benthamiana by transient expression. Plant-produced SARS-CoV-2 RBD-Fc, Alpha RBD-Fc and Beta RBD-Fc exhibited specific binding to human angiotensin converting enzyme 2 (ACE2) receptor determined by ELISA. Intriguingly, the binding of plant-produced SARS-CoV-2 RBD proteins to plant-produced mAbs CR3022, B38, and H4 was found to be different depending on the variant mutation. In contrary to the plant-produced SARS-CoV-2 RBD-Fc and Alpha RBD-Fc, Beta RBD-Fc variant showed weak binding affinity towards the mAbs. The result suggested that the Beta RBD variant might have acquired partial resistance to neutralizing antibodies compared to other variants. However, further studies with sera from convalescent or vaccinated individuals are required to confirm this finding.
format	article
author	Kaewta Rattanapisit Christine Joy I Bulaon Narach Khorattanakulchai Balamurugan Shanmugaraj Kittikhun Wangkanont Waranyoo Phoolcharoen
author_facet	Kaewta Rattanapisit Christine Joy I Bulaon Narach Khorattanakulchai Balamurugan Shanmugaraj Kittikhun Wangkanont Waranyoo Phoolcharoen
author_sort	Kaewta Rattanapisit
title	Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
title_short	Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
title_full	Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
title_fullStr	Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
title_full_unstemmed	Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
title_sort	plant-produced sars-cov-2 receptor binding domain (rbd) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c9a613561f4c42e29dd70651076412cb
work_keys_str_mv	AT kaewtarattanapisit plantproducedsarscov2receptorbindingdomainrbdvariantsshoweddifferentialbindingefficiencywithantispikespecificmonoclonalantibodies AT christinejoyibulaon plantproducedsarscov2receptorbindingdomainrbdvariantsshoweddifferentialbindingefficiencywithantispikespecificmonoclonalantibodies AT narachkhorattanakulchai plantproducedsarscov2receptorbindingdomainrbdvariantsshoweddifferentialbindingefficiencywithantispikespecificmonoclonalantibodies AT balamuruganshanmugaraj plantproducedsarscov2receptorbindingdomainrbdvariantsshoweddifferentialbindingefficiencywithantispikespecificmonoclonalantibodies AT kittikhunwangkanont plantproducedsarscov2receptorbindingdomainrbdvariantsshoweddifferentialbindingefficiencywithantispikespecificmonoclonalantibodies AT waranyoophoolcharoen plantproducedsarscov2receptorbindingdomainrbdvariantsshoweddifferentialbindingefficiencywithantispikespecificmonoclonalantibodies
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Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.

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