Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)

The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.

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Detalles Bibliográficos
Autores principales: Benjamin Carter, Wai Lim Ku, Jee Youn Kang, Gangqing Hu, Jonathan Perrie, Qingsong Tang, Keji Zhao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/c9a8a9af0a7e477299b738bebf2bf047
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Sumario:The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.