Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)

The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.

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Autores principales:	Benjamin Carter, Wai Lim Ku, Jee Youn Kang, Gangqing Hu, Jonathan Perrie, Qingsong Tang, Keji Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c9a8a9af0a7e477299b738bebf2bf047
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spelling	oai:doaj.org-article:c9a8a9af0a7e477299b738bebf2bf0472021-12-02T14:38:40ZMapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)10.1038/s41467-019-11559-12041-1723https://doaj.org/article/c9a8a9af0a7e477299b738bebf2bf0472019-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11559-1https://doaj.org/toc/2041-1723The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.Benjamin CarterWai Lim KuJee Youn KangGangqing HuJonathan PerrieQingsong TangKeji ZhaoNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Benjamin Carter Wai Lim Ku Jee Youn Kang Gangqing Hu Jonathan Perrie Qingsong Tang Keji Zhao Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
description	The authors introduce ACT-seq: a Tn5-based method for rapidly profiling epigenetic marks in bulk-cell and single-cell samples. ACT-seq avoids many laborious or time-consuming steps required for similar techniques including chromatin fragmentation and end repair.
format	article
author	Benjamin Carter Wai Lim Ku Jee Youn Kang Gangqing Hu Jonathan Perrie Qingsong Tang Keji Zhao
author_facet	Benjamin Carter Wai Lim Ku Jee Youn Kang Gangqing Hu Jonathan Perrie Qingsong Tang Keji Zhao
author_sort	Benjamin Carter
title	Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
title_short	Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
title_full	Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
title_fullStr	Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
title_full_unstemmed	Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)
title_sort	mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (act-seq)
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c9a8a9af0a7e477299b738bebf2bf047
work_keys_str_mv	AT benjamincarter mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq AT wailimku mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq AT jeeyounkang mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq AT gangqinghu mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq AT jonathanperrie mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq AT qingsongtang mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq AT kejizhao mappinghistonemodificationsinlowcellnumberandsinglecellsusingantibodyguidedchromatintagmentationactseq
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Mapping histone modifications in low cell number and single cells using antibody-guided chromatin tagmentation (ACT-seq)

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